RESUMEN
The motoneural control of skeletal muscle contraction requires the neuromuscular junction (NMJ), a midmuscle synapse between the motor nerve and myotube. The formation and maintenance of NMJs are orchestrated by the muscle-specific receptor tyrosine kinase (MuSK). Motor neuron-derived agrin activates MuSK via binding to MuSK's coreceptor Lrp4, and genetic defects in agrin underlie a congenital myasthenic syndrome (an NMJ disorder). However, MuSK-dependent postsynaptic differentiation of NMJs occurs in the absence of a motor neuron, indicating a need for nerve/agrin-independent MuSK activation. We previously identified the muscle protein Dok-7 as an essential activator of MuSK. Although NMJ formation requires agrin under physiological conditions, it is dispensable for NMJ formation experimentally in the absence of the neurotransmitter acetylcholine, which inhibits postsynaptic specialization. Thus, it was hypothesized that MuSK needs agrin together with Lrp4 and Dok-7 to achieve sufficient activation to surmount inhibition by acetylcholine. Here, we show that forced expression of Dok-7 in muscle enhanced MuSK activation in mice lacking agrin or Lrp4 and restored midmuscle NMJ formation in agrin-deficient mice, but not in Lrp4-deficient mice, probably due to the loss of Lrp4-dependent presynaptic differentiation. However, these NMJs in agrin-deficient mice rapidly disappeared after birth, and postsynaptic specializations emerged ectopically throughout myotubes whereas exogenous Dok-7-mediated MuSK activation was maintained. These findings demonstrate that the MuSK activator agrin plays another role essential for the postnatal maintenance, but not for embryonic formation, of NMJs and also for the postnatal, but not prenatal, midmuscle localization of postsynaptic specializations, providing physiological and pathophysiological insight into NMJ homeostasis.
Asunto(s)
Agrina/fisiología , Unión Neuromuscular/enzimología , Proteínas Tirosina Quinasas Receptoras/metabolismo , Agrina/deficiencia , Agrina/genética , Empalme Alternativo , Animales , Diafragma/embriología , Diafragma/crecimiento & desarrollo , Activación Enzimática , Femenino , Proteínas Relacionadas con Receptor de LDL , Longevidad/genética , Masculino , Ratones , Ratones Transgénicos , Fibras Musculares Esqueléticas/enzimología , Fibras Musculares Esqueléticas/fisiología , Proteínas Musculares/deficiencia , Proteínas Musculares/fisiología , Unión Neuromuscular/embriología , Unión Neuromuscular/crecimiento & desarrollo , Enfermedades de la Unión Neuromuscular/enzimología , Enfermedades de la Unión Neuromuscular/genética , Enfermedades de la Unión Neuromuscular/fisiopatología , Fosforilación , Densidad Postsináptica/fisiología , Procesamiento Proteico-Postraduccional , Receptores Colinérgicos/fisiología , Receptores de LDL/deficiencia , Receptores de LDL/fisiología , Proteínas Recombinantes de Fusión/metabolismo , Prueba de Desempeño de Rotación con Aceleración ConstanteRESUMEN
Diabetic neuropathy is associated with functional and morphological changes of the neuromuscular junction (NMJ) associated with muscle weakness. This study examines the effect of type 1 diabetes on NMJ function. Swiss Webster mice were made diabetic with three interdaily ip injections of streptozotocin (STZ). Mice were severely hyperglycemic within 7 days after the STZ treatment began. Whereas performance of mice on a rotating rod remained normal, the twitch tension response of the isolated extensor digitorum longus to nerve stimulation was reduced significantly at 4 wk after the onset of STZ-induced hyperglycemia. This mechanical alteration was associated with increased amplitude and prolonged duration of miniature end-plate currents (mEPCs). Prolongation of mEPCs was not due to expression of the embryonic acetylcholine receptor but to reduced muscle expression of acetylcholine esterase (AChE). Greater sensitivity of mEPC decay time to the selective butyrylcholinesterase (BChE) inhibitor PEC suggests that muscle attempts to compensate for reduced AChE levels by increasing expression of BChE. These alterations of AChE are attributed to STZ-induced hyperglycemia since similar mEPC prolongation and reduced AChE expression were found for db/db mice. The reduction of muscle end-plate AChE activity early during the onset of STZ-induced hyperglycemia may contribute to endplate pathology and subsequent muscle weakness during diabetes.
Asunto(s)
Acetilcolinesterasa/deficiencia , Diabetes Mellitus Experimental/enzimología , Diabetes Mellitus Tipo 1/enzimología , Neuropatías Diabéticas/enzimología , Enfermedades de la Unión Neuromuscular/enzimología , Acetilcolinesterasa/biosíntesis , Animales , Butirilcolinesterasa/biosíntesis , Inhibidores de la Colinesterasa/farmacología , Neuropatías Diabéticas/fisiopatología , Proteínas Ligadas a GPI/biosíntesis , Proteínas Ligadas a GPI/deficiencia , Hiperglucemia/enzimología , Hiperglucemia/fisiopatología , Masculino , Ratones , Placa Motora/enzimología , Placa Motora/fisiopatología , Debilidad Muscular/enzimología , Debilidad Muscular/fisiopatología , Enfermedades de la Unión Neuromuscular/fisiopatología , Fisostigmina/análogos & derivados , Fisostigmina/farmacologíaRESUMEN
The role of the conserved focal adhesion kinase (FAK) family of protein tyrosine kinases in the development and physiological functions of the CNS has long been an area of interest among neuroscientists. In this report, we observe that Drosophila mutants lacking Fak56 exhibit a decreased lifespan, accompanied by a bang-sensitive phenotype, which is characterized by sensitivity to mechanical and high-frequency electrical stimulation. Fak56 mutant animals display lower thresholds and higher rates of seizures in response to electroconvulsive stimuli. Direct measurements of action potential conduction in larval segmental nerves demonstrate a slowed propagation speed and failure during high-frequency nerve stimulation. In addition, neuromuscular junctions in Fak56 mutant animals display transmission blockade during high-frequency activity as a result of action potential failure. Endogenous Fak56 protein is abundant in glial cells ensheathing the axon bundles, and structural alterations of segmental nerve bundles can be observed in mutants. Manipulation of Fak56 function specifically in glial cells also disrupts action potential conduction and neurotransmission, suggesting a glial component in the Fak56 bang-sensitive phenotype. Furthermore, we show that increased intracellular calcium levels result in the dephosphorylation of endogenous Fak56 protein in Drosophila cell lines, in parallel with our observations of highly variable synaptic potentials at a higher Ca2+ level in Fak56 mutant larvae. Together these findings suggest that modulation of Fak56 function is important for action potential propagation and Ca2+-regulated neuromuscular transmission in vivo.
Asunto(s)
Proteínas de Drosophila/genética , Quinasa 1 de Adhesión Focal/genética , Reacción Cataléptica de Congelación/fisiología , Sistema Nervioso/enzimología , Sistema Nervioso/crecimiento & desarrollo , Enfermedades de la Unión Neuromuscular/enzimología , Transmisión Sináptica/genética , Potenciales de Acción/genética , Animales , Axones/enzimología , Axones/ultraestructura , Señalización del Calcio/genética , Células Cultivadas , Drosophila melanogaster , Estimulación Eléctrica , Femenino , Predisposición Genética a la Enfermedad/genética , Longevidad/genética , Masculino , Mutación/genética , Conducción Nerviosa/genética , Neuroglía/enzimología , Neuroglía/ultraestructura , Unión Neuromuscular/genética , Unión Neuromuscular/metabolismo , Unión Neuromuscular/fisiopatología , Enfermedades de la Unión Neuromuscular/genética , Enfermedades de la Unión Neuromuscular/fisiopatología , Convulsiones/genética , Convulsiones/metabolismo , Convulsiones/fisiopatología , Estrés MecánicoRESUMEN
Acetylcholinesterase (AChE) plays an essential role in neuromuscular transmission, therefore it is surprising that AChE knockout (KO) mice could live to the adulthood. Neuromuscular functioning in KO and normal (wild type, WT) mice were studied, at different age (1.5-, 4- and 9-month-old). Hindlimb muscle force productions in response to nerve or muscle electric stimulation were recorded in situ and in vitro. Our results show that contrary to WT mice, 1.5-, 4- and 9-month-old KO mice exhibited a decreased in tetanic force during short periods (500 ms) of repetitive nerve stimulations (tetanic fade). Nevertheless submaximal muscle forces in response to single or repetitive nerve stimulation were increased (potentiation) in 1.5-, 4- and 9-month-old KO mice as compared to WT mice (p<0.05). Tetanic fade and potentiation were absent when muscles were directly stimulated, indicating neuromuscular transmission alterations in KO mice. Contrary to younger mice, muscle weight and maximal tetanic force in response to repetitive nerve stimulation were not reduced in 4- and 9-month-old KO mice as compared to WT mice (p>0.05). In conclusion AChE deficit leads to marked neuromuscular alterations in hind limb muscle functioning and a prominent symptom is the lack of resistance to fatigue.