RESUMEN
BACKGROUND: Dorema aucheri gum (DAG) is a bitter flavonoid gum widely used for numerous medicinal purposes including wound recovery. The present work investigates the acute toxicity and wound-healing effects of DAG in excisional skin injury in rats. MATERIALS AND METHODS: Sprague Dawley rats (24) were clustered into four groups, each rat had a full-thickness excisional dorsal neck injury (2.00 cm) and addressed with 0.2 mL of the following treatments for 15 days: Group A (vehicle), rats addressed with normal saline; Group B, rats received intrasite gel; C and D, rats addressed with 250 and 500 mg/kg of DAG, respectively. RESULTS: The results revealed the absence of any toxic signs in rats who received oral dosages of 2 and 5 g/kg of DAG. Wound healing was significantly accelerated following DAG treatments indicated by smaller open areas and higher wound contraction percentages compared to vehicle rats. Histological evaluation revealed higher fibroblast formation, collagen deposition, and noticeably lower inflammatory cell infiltration in granulated skin tissues of DAG-addressed rats compared to vehicle rats. DAG treatment caused significant modulation of immunohistochemical proteins (decreased Bax and increased HSP 70) and inflammatory mediators (reduced TNF-α, IL-6, and magnified IL-10), which were significantly varied compared to vehicle rats. Moreover, topical DAG treatment led to significant upregulation of the hydroxyproline (HDX) (collagen) and antioxidant content. At the same time, decreased the lipid peroxidation (MDA) levels in healed tissues obtained from DAG-treated rats. CONCLUSION: The present wound contraction by DAG might be linked with the modulatory effect of its phytochemicals (polysaccharides, flavonoids, and phenolic) on the cellular mechanisms, which justify their folkloric use and provokes further investigation as therapeutic drug additives for wound contraction.
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Flavonoides , Ratas Sprague-Dawley , Piel , Cicatrización de Heridas , Proteína X Asociada a bcl-2 , Animales , Cicatrización de Heridas/efectos de los fármacos , Ratas , Flavonoides/farmacología , Piel/efectos de los fármacos , Piel/lesiones , Piel/patología , Piel/metabolismo , Proteína X Asociada a bcl-2/metabolismo , Proteínas HSP70 de Choque Térmico/metabolismo , Hidroxiprolina/metabolismo , Masculino , Gomas de Plantas/farmacologíaRESUMEN
The understanding of cancer immunity and antitumor factors generated by natural polysaccharides is not yet fully comprehended. Polysaccharides, like cashew gum (CG), can exhibit immunomodulatory action and may assist in the antitumor process and side effects relieve. This study aimed to determine the antitumor effect of CG alone or in combination with cyclophosphamide (CTX), and its interactions with immune cells, in a murine melanoma model, using the B16-F10 cell line. Tumor growth inhibition, hematological, histopathological, ELISA, flow cytometry, immunofluorescence, and qRT-PCR analyses were performed to elucidate the antitumor potential, involvement of immune cells, and potential toxic effects. CG showed significant tumor growth inhibition, reaching up to 42.9 % alone and 51.4 % in combination with CTX, with mild toxicity to organs. CG enhanced leukocyte count, even in the presence of CTX. Furthermore, CG influenced the activation of tumor-associated macrophages (TAM), characterized by an increase in Il4, as well as a reduction in Ifng, Il1b, Tgfb, and Il6 gene expression. Nevertheless, these effects did not compromise the antitumor activity of CG. In summary, the combination of CG with CTX is a promising approach for leukopenia, one of the most important side effects of cancer treatment and deserves further investigation.
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Anacardium , Ciclofosfamida , Melanoma Experimental , Animales , Ciclofosfamida/farmacología , Ratones , Melanoma Experimental/tratamiento farmacológico , Melanoma Experimental/patología , Anacardium/química , Gomas de Plantas/química , Gomas de Plantas/farmacología , Línea Celular Tumoral , Modelos Animales de Enfermedad , Citocinas/metabolismo , Macrófagos Asociados a Tumores/efectos de los fármacos , Macrófagos Asociados a Tumores/inmunologíaRESUMEN
Herein, the design and fabrication of an anticancer nanoplatform (LBG/PRA-NG) based on locust bean gum-stabilized nanogold and functionalized with Phyllanthus reticulatus anthocyanins was described. LBG/PRA-NG was prepared in an eco-friendly, one-pot approach at room temperature, mediated by the anthocyanins and gum as bio-reductant and stabilizer, respectively. The nanostructure was elaborately characterized by FESEM, TEM, UV-visible, DLS, Zeta potential, FTIR, XRD, TGA/DTG, and XPS analysis. Its anticancer attributes were examined based on cytotoxicity on MCF-7 and MDA-MB-231 breast cancer cell lines, as well as the generation of intracellular reactive oxygen species. The results revealed the successful formation of a homogenous and highly stable nanocomposite (LBG/PRA-NG), with quasi-spherical shape, small size (14.73 nm), Zeta potential and PDI values of -58.30 mV and 0.237, respectively. The presence of a plasmonic peak at 525 nm was indicative of AuNPs. Compared to the galactomannan and anthocyanin, LBG/PRA-NG exhibited superior antioxidative properties with IC50 values of 35.44 µg/mL against DPPH and 24.55 µg/mL against ABTS+. Notably, LBG/PRA-NG also demonstrated enhanced anticancer properties relative to LBG and anthocyanins, with IC50 values of 16.17 µg/mL and 25.06 µg/mL against MCF-7 and MDA-MB-231 cells. Meanwhile, the normal cells (HEK-293 and L929) resisted the innocuous effects of LBG/PRA-NG. Furthermore, treatment of breast cancer cells with LBG/PRA-NG drastically elevated the intracellular ROS levels. This suggested that the anticancer activity of LBG/PRA-NG may be mediated via amplification of ROS/oxidative stress-induced apoptosis. Altogether, these findings indicate the remarkable potential of LBG/PRA-NC in the development of anticancer therapy.
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Antocianinas , Antineoplásicos , Antioxidantes , Galactanos , Oro , Mananos , Nanopartículas del Metal , Gomas de Plantas , Humanos , Gomas de Plantas/química , Gomas de Plantas/farmacología , Mananos/química , Mananos/farmacología , Antocianinas/química , Antocianinas/farmacología , Galactanos/química , Galactanos/farmacología , Antioxidantes/farmacología , Antioxidantes/química , Oro/química , Oro/farmacología , Antineoplásicos/farmacología , Antineoplásicos/química , Nanopartículas del Metal/química , Células MCF-7 , Especies Reactivas de Oxígeno/metabolismo , Línea Celular Tumoral , Nanocompuestos/químicaRESUMEN
Functionally distinct dietary fibre sources may be combined in reformulated foods to restore a natural spectrum of health attributes. Effects of wheat bran (WB), psyllium husk, guar gum and Raftilose™ combinations on hydrated faecal mass (HFM), were determined. A valid rat model was fed diets supplemented with 10% WB, 10% WB with 1-6% psyllium in 1% steps, and 10% WB/5% psyllium with 1-7% guar gum or 1-6% Raftilose in 1% steps. Fully hydrated faecal pellets gave HFM values in the human range, increasing by 2.4 ± 0.29 g per gram of WB ingested, and by 15.6 ± 1.52 g per g of psyllium. Equations for incremental changes in HFM predicted intakes of fibre combinations required for adequate daily HFM, and it is shown how expressing relative effects of foods on HFM as functional equivalents would allow quantitative personalised management of HFM for reduced constipation and colorectal cancer in humans.
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Fibras de la Dieta , Heces , Galactanos , Mananos , Gomas de Plantas , Psyllium , Fibras de la Dieta/farmacología , Animales , Heces/química , Humanos , Mananos/farmacología , Gomas de Plantas/farmacología , Galactanos/farmacología , Ratas , Psyllium/farmacología , Masculino , Ratas Sprague-Dawley , Estreñimiento/dietoterapia , Modelos AnimalesRESUMEN
Addressing the drawbacks of traditional flocculants on microalgae biomass harvesting is crucial for large-scale industrial applications of microalgae production. In this study, cationic bioflocculant was successfully prepared by introducing cationic groups into the side chain of guar gum, achieving in-situ algae flocculation efficiency of 83.5 % with the dosage of 18.0 mg/L under pH = 10.0. Through a harmonious integration of predictive modelling and practical experimentation, a superior cell flocculation capacity of 23.5 g/g was achieved. In addition, the environmental safety and biocompatibility of cationic guar gum was assessed, using the typical suspension quantitative bacteriostatic method and the fluorescent double-staining technique. The results showed that the inhibition efficiency of Staphylococcus aureus in the system containing 60.0 mg/L cationic guar gum was only 12.0 % and there was no inhibition against Escherichia coli colonies. These findings provide a safe and green flocculant for efficient microalgae harvesting and spent medium treatment.
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Cationes , Floculación , Galactanos , Mananos , Microalgas , Gomas de Plantas , Galactanos/farmacología , Galactanos/química , Gomas de Plantas/química , Gomas de Plantas/farmacología , Floculación/efectos de los fármacos , Mananos/farmacología , Mananos/química , Microalgas/efectos de los fármacos , Staphylococcus aureus/efectos de los fármacos , Escherichia coli/efectos de los fármacos , Concentración de Iones de HidrógenoRESUMEN
Preventing bacterial infections is a crucial aspect of wound healing. There is an urgent need for multifunctional biomaterials without antibiotics to promote wound healing. In this study, we fabricated a guar gum (GG)-based nanocomposite hydrogel, termed GBTF, which exhibited photothermal antibacterial therapy for infected wound healing. The GBTF hydrogel formed a cross-linked network through dynamic borate/diol interactions between GG and borax, thereby exhibiting simultaneously self-healing, adaptable, and injectable properties. Additionally, tannic acid (TA)/Fe3+ nanocomplexes (NCs) were incorporated into the hydrogel to confer photothermal antibacterial properties. Under the irradiation of an 808 nm near-infrared laser, the TA/Fe3+ NCs in the hydrogel could rapidly generate heat, leading to the disruption of bacterial cell membranes and subsequent bacterial eradication. Furthermore, the hydrogels exhibited good cytocompatibility and hemocompatibility, making them a precandidate for preclinical and clinical applications. Finally, they could significantly promote bacteria-infected wound healing by reducing bacterial viability, accelerating collagen deposition, and promoting epithelial remodeling. Therefore, the multifunctional GBTF hydrogel, which was composed entirely of natural substances including guar gum, borax, and polyphenol/ferric ion NCs, showed great potential for regenerating infected skin wounds in clinical applications.
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Antibacterianos , Galactanos , Hidrogeles , Mananos , Nanocompuestos , Terapia Fototérmica , Gomas de Plantas , Cicatrización de Heridas , Mananos/química , Mananos/farmacología , Gomas de Plantas/química , Gomas de Plantas/farmacología , Galactanos/química , Galactanos/farmacología , Cicatrización de Heridas/efectos de los fármacos , Antibacterianos/farmacología , Antibacterianos/química , Nanocompuestos/química , Hidrogeles/química , Hidrogeles/farmacología , Animales , Terapia Fototérmica/métodos , Ratones , Taninos/química , Taninos/farmacología , Staphylococcus aureus/efectos de los fármacos , Humanos , Escherichia coli/efectos de los fármacos , BoratosRESUMEN
Five new characteristic cembrane-type diterpenoids (olibacartiols A-E, 1-5) were acquired from the gum resin of Boswellia carterii. The structures of these diterpenoids were characterized by detailed spectroscopic analysis, and compounds 1-3 were unambiguously confirmed by single-crystal X-ray diffraction experiments. The anti-inflammatory activities of the isolated compounds were evaluated using LPS-induced BV2 cell model and compounds 2-5 showed moderate NO inhibitory effects with IC50 values of 8.84 ± 1.02, 9.82 ± 1.95, 9.75 ± 2.24, and 7.39 ± 1.24 µM, respectively.
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Antiinflamatorios , Boswellia , Diterpenos , Óxido Nítrico , Fitoquímicos , Resinas de Plantas , Diterpenos/farmacología , Diterpenos/aislamiento & purificación , Diterpenos/química , Boswellia/química , Óxido Nítrico/metabolismo , Estructura Molecular , Animales , Antiinflamatorios/farmacología , Antiinflamatorios/aislamiento & purificación , Antiinflamatorios/química , Resinas de Plantas/química , Ratones , Fitoquímicos/farmacología , Fitoquímicos/aislamiento & purificación , Línea Celular , China , Gomas de Plantas/química , Gomas de Plantas/farmacologíaRESUMEN
The consumption of functional foods in a daily diet is a promising approach for the maintenance of cognitive health. The present study examines the effects of water-soluble prebiotic dietary-fiber, partially hydrolyzed guar gum (PHGG), on cognitive function and mental health in healthy elderly individuals. Participants consumed either 5 g/day of PHGG or a placebo daily for 12 weeks in this randomized, double-blind, placebo-controlled, and parallel-group study. An assessment of cognitive functions, sleep quality, and subjective mood evaluations was performed at baseline and after 8 and 12 weeks of either PHGG or placebo intake. The visual memory scores in cognitive function tests and sleepiness on rising scores related to sleep quality were significantly improved in the PHGG group compared to the placebo group. No significant differences were observed in mood parameters between the groups. Vigor-activity scores were significantly improved, while the scores for Confusion-Bewilderment decreased significantly in the PHGG group when compared to the baseline. In summary, supplementation with PHGG was effective in improving cognitive functions, particularly visual memory, as well as enhancing sleep quality and vitality in healthy elderly individuals (UMIN000049070).
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Cognición , Galactanos , Mananos , Gomas de Plantas , Humanos , Galactanos/farmacología , Mananos/farmacología , Mananos/administración & dosificación , Gomas de Plantas/farmacología , Método Doble Ciego , Cognición/efectos de los fármacos , Anciano , Masculino , Femenino , Sueño/efectos de los fármacos , Prebióticos/administración & dosificación , Calidad del Sueño , Fibras de la Dieta/farmacología , Fibras de la Dieta/administración & dosificación , Hidrólisis , Memoria/efectos de los fármacos , Suplementos Dietéticos , Persona de Mediana Edad , Voluntarios Sanos , Afecto/efectos de los fármacosRESUMEN
This study evaluated the nutritional profile and fiber content of innovative formulations of wheat-based biscuits enriched with chia seeds, carob flour and coconut sugar. The in vitro antioxidant, cytotoxic, anti-inflammatory and antimicrobial activities were also investigated to understand the potential health advantages of the incorporation of these new ingredients. The novel biscuits demonstrated significant improvements in protein and mineral content, with increases of 50% and 100% in chia biscuits, and up to 20% and 40% in carob biscuits, respectively. Fiber also notably increased, particularly in samples containing 10% carob flour, which increased four times as compared to wheat-based samples. The new ingredients exhibited antibacterial and antifungal activity, particularly against Yersinia enterocolitica (minimum inhibitory concentration 1.25 mg mL-1 in coconut sugar) and Aspergillus fumigatus (minimum inhibitory concentration/minimum fungicidal concentrations 2.5/5 mg mL-1 in chia seeds). However, the final biscuits only displayed antifungal properties. Carob flour and chia seeds had a remarkably high capacity to inhibit the formation of TBARS and promoted greater antioxidant activity in biscuit formulations, with EC50 values decreasing from 23.25 mg mL-1 (control) to 4.54 mg mL-1 (15% defatted ground chia seeds) and 1.19 mg mL-1 (10% carob flour). Only chia seeds exhibited cellular antioxidant, anti-inflammatory and cytotoxic activity, attributes that were lost when seeds were added into the biscuits. These findings highlight the potential health benefits of these ingredients, particularly when incorporated in new wheat-based formulations.
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Antioxidantes , Fibras de la Dieta , Harina , Galactanos , Mananos , Valor Nutritivo , Triticum , Triticum/química , Fibras de la Dieta/análisis , Fibras de la Dieta/farmacología , Antioxidantes/farmacología , Antioxidantes/química , Harina/análisis , Semillas/química , Gomas de Plantas/química , Gomas de Plantas/farmacología , Antiinfecciosos/farmacología , Salvia/química , beta-Glucanos/farmacología , beta-Glucanos/química , Antiinflamatorios/farmacología , Antiinflamatorios/química , Pan/análisis , Antibacterianos/farmacología , Antibacterianos/química , Goma Arábiga/química , Goma Arábiga/farmacología , Animales , Pruebas de Sensibilidad Microbiana , HumanosRESUMEN
BACKGROUND: Guggulipid, an oleo-gum resin extracted from the bark of Commiphora wightii of the Burseraceae family, holds a significant place in Ayurvedic medicine due to its historical use in treating various disorders, including inflammation, gout, rheumatism, obesity, and lipid metabolism imbalances. OBJECTIVE: This comprehensive review aims to elucidate the molecular targets of guggulipids and explore their cellular responses. Furthermore, it summarizes the findings from in-vitro, in-vivo, and clinical investigations related to arthritis and various inflammatory conditions. METHODS: A comprehensive survey encompassing in-vitro, in-vivo, and clinical studies has been conducted to explore the therapeutic capacity of guggulipid in the management of rheumatoid arthritis. Various molecular pathways, such as cyclooxygenase-2 (COX-2), vascular endothelial growth factor (VEGF), PI3-kinase/AKT, JAK/STAT, nitric oxide synthase (iNOS), and NFκB signaling pathways, have been targeted to assess the antiarthritic and anti-inflammatory effects of this compound. RESULTS: The research findings reveal that guggulipid demonstrates notable antiarthritic and anti-inflammatory effects by targeting key molecular pathways involved in inflammatory responses. These pathways include COX-2, VEGF, PI3-kinase/AKT, JAK/STAT, iNOS, and NFκB signaling pathways. in-vitro, in-vivo, and clinical studies collectively support the therapeutic potential of guggulipid in managing rheumatoid arthritis and related inflammatory conditions. CONCLUSION: This review provides a deeper understanding of the therapeutic mechanisms and potential of guggulipid in the management of rheumatoid arthritis. The collective evidence strongly supports the promising role of guggulipid as a therapeutic agent, encouraging further research and development in guggulipid-based treatments for these conditions.
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Artritis Reumatoide , Commiphora , Extractos Vegetales , Gomas de Plantas , Artritis Reumatoide/tratamiento farmacológico , Humanos , Gomas de Plantas/uso terapéutico , Gomas de Plantas/farmacología , Extractos Vegetales/uso terapéutico , Extractos Vegetales/farmacología , Animales , Antiinflamatorios/uso terapéutico , Antiinflamatorios/farmacología , Antirreumáticos/uso terapéutico , Antirreumáticos/farmacologíaRESUMEN
Partially hydrolyzed guar gum (PHGG) is a soluble dietary fiber that is effective for defecation control. It influences the gut microbiota, by which it is metabolized to yield short-chain fatty acids (SCFAs), and it was also recently shown to protect against influenza infection in humans. We here investigated the effects of PHGG in a mouse model of influenza H1N1 virus infection. Eight-week-old C57BL/6 mice were fed normal chow with or without PHGG (500 mg/kg per day) for 4 weeks, infected with H1N1 at 10 weeks of age, and analyzed at 12 weeks of age. Administration of PHGG attenuated the decline in body weight induced by H1N1 infection without affecting food intake. It also ameliorated intestinal atrophy and increased the production of SCFAs including acetic acid, propionic acid, and butyric acid in the cecum, thereby preventing the inhibitory effect of H1N1 infection on SCFA production. The H1N1-induced increases in the serum concentrations of inflammatory cytokines including interferon-γ and interleukin-6 and anti-inflammatory cytokine such as interleukin-10 were all inhibited by PHGG intake. In addition, PHGG administration attenuated inflammatory gene expression in the lung and promoted both natural killer cell activity and regulatory T-cell differentiation in the spleen. Our findings suggest that the consumption of PHGG may improve the gut environment and thereby limit the inflammatory response to H1N1 infection. They may thus provide the basis for novel dietary intervention strategies to suppress the excessive inflammation associated with virus infection.
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Enfermedades Transmisibles , Microbioma Gastrointestinal , Subtipo H1N1 del Virus de la Influenza A , Gripe Humana , Infecciones por Orthomyxoviridae , Humanos , Ratones , Animales , Ratones Endogámicos C57BL , Inflamación/tratamiento farmacológico , Inflamación/metabolismo , Galactanos/farmacología , Mananos/farmacología , Gomas de Plantas/farmacología , Ácidos Grasos Volátiles/metabolismo , Fibras de la Dieta/farmacologíaRESUMEN
BACKGROUND: The intestinal epithelium acts as a barrier against harmful luminal materials, thus preventing intestinal diseases and maintaining intestinal health. Heat shock protein 27 (HSP27) promotes intestinal epithelial integrity under both physiological and stressed conditions. The effects of partially hydrolyzed guar gum (PHGG) on HSP27 expression in intestinal Caco-2 cells and mouse intestines were investigated. RESULTS: The present study showed that PHGG upregulated HSP27 expression in Caco-2 cells without upregulating Hspb1, the gene encoding HSP27. Feeding PHGG increased HSP25 expression in epithelial cells of the small intestine of mice. Inhibition of protein translation using cycloheximide suppressed PHGG-mediated HSP27 expression, indicating that PHGG upregulated HSP27 via translational modulation. Signaling inhibition of the mechanistic target of rapamycin (mTOR) and phosphatidyl 3-inositol kinase reduced PHGG-mediated HSP27 expression, whereas mitogen-activated protein kinase kinase inhibition by U0126 increased HSP27 expression, irrespective of PHGG administration. PHGG increases mTOR phosphorylation and reduces extracellular signal-regulated protein kinase (ERK) phosphorylation. CONCLUSION: PHGG-mediated translation of HSP27 in intestinal Caco-2 cells and mouse intestine via the mTOR and ERK signaling pathways may promote intestinal epithelial integrity. These findings help us better understand how dietary fibers regulate the physiological function of the intestines. © 2023 Society of Chemical Industry.
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Proteínas de Choque Térmico HSP27 , Intestinos , Humanos , Ratones , Animales , Células CACO-2 , Proteínas de Choque Térmico HSP27/genética , Galactanos/farmacología , Mananos/farmacología , Gomas de Plantas/farmacología , Serina-Treonina Quinasas TOR/genéticaRESUMEN
The aim of the present work was to modify the exuded gum of Sterculia striata tree by an amination reaction. The viscosity and zero potential of the chicha gum varied as a function of pH. The modification was confirmed by X-ray diffraction (XRD), infrared spectroscopy (FTIR), size exclusion chromatography (SEC), zeta potential, thermogravimetric analysis (TG), and differential scanning calorimetry (DSC). Furthermore, the chemical modification changed the molar mass and surface charge of the chicha gum. In addition, the gums were used in tests for ex vivo mucoadhesion strength, antibacterial activity against the standard strain of Staphylococcus aureus (ATCC 25923), inhibitory activity of α-glucosidase, antioxidant capacity, and viability of Caco-2 cells. Through these tests, it was found that amination caused an increase in the mucoadhesive and inhibitory activity of chicha gum against the bacterium Staphylococcus aureus. In addition, the gums (pure and modified) showed antioxidant capacity and an inhibitory effect against the α-glucosidase enzyme and did not show cytotoxic potential.
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Antioxidantes , alfa-Glucosidasas , Humanos , Antioxidantes/farmacología , Células CACO-2 , Antibacterianos/farmacología , Antibacterianos/química , Difracción de Rayos X , Gomas de Plantas/farmacología , Gomas de Plantas/química , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
A 56-day feeding trial was conducted to assess the effects of different viscous guar gum on the growth, intestinal flora, and intestinal health of Micropterus salmoides. Four practical diets with 42.5 % crude protein and 13.7 % crude lipid were formulated to contain 8 % cellulose and three different viscosities (2500, 5200, and 6000 mPa·s) of guar gum. Dietary guar gum inhibits fish growth and feed utilization, decreases the α-diversity of the intestinal flora, and negatively alters the intestinal flora structure and metabolite composition. High viscous guar gum down-regulated the intestinal tight junction, anti-inflammatory, and anti-apoptotic related gene's expression, decreased digesta butyrate/histamine ratio; and increased the abundance of Plesiomonas shigelloides. These results suggest that dietary guar gum adversely affects intestinal health by disrupting intestinal flora structure and metabolite composition, and that viscosity should be considered when using guar gum as a binder in aquafeeds.
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Lubina , Microbioma Gastrointestinal , Animales , Viscosidad , Galactanos/química , Gomas de Plantas/farmacología , Gomas de Plantas/química , Mananos/química , Fibras de la DietaRESUMEN
Plant gums are bio-organic substances that are derived from the barks of trees. They are biodegradable and non-adverse complex polysaccharides that have been gaining usage in recent years due to a number of advantages they contribute to various applications. In this study, gum was collected from Moringa oleifera and Azadirachta indica trees, then dried and powdered. Characterizations of gum polysaccharides were performed using TLC, GC-MS, NMR, etc., and sugar molecules such as glucose and xylose were found to be present. Effects of the gums on Abelmoschus esculentus growth were observed through root growth, shoot growth, and biomass content. The exposure of the seeds to the plant gums led to bio stimulation in the growth of the plants. Poor quality soil was exposed to the gum polysaccharide, where the polysaccharide was found to improve soil quality, which was observed through soil analysis and SEM analysis of soil porosity and structure. Furthermore, the plant gums were also found to have bio-pesticidal activity against mealybugs, which showed certain interstitial damage evident through histopathological analysis.
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Azadirachta , Moringa oleifera , Plaguicidas , Moringa oleifera/química , Gomas de Plantas/química , Gomas de Plantas/farmacología , Plantas , Polisacáridos/química , Polisacáridos/farmacología , SueloRESUMEN
Nosocomial bacterial infections associated with biofilms inspire to explore newer bactericidal strategy with eco-friendly biomaterials as sustainable alternatives. In this research work, we successfully developed bio-safe films from kojic acid(KA) and guar gum propionate(GGP) for Escherichia coli biofilm disruption and planktonic cell killing. High DS(degree of substitution = 1.52) GGP was synthesized from guar gum (GG)assisted by chaotropic ions at room-temperature. Biopolymers were routinely characterized in CHN analyzer, FT-IR, TGA and XRD analysis. KA loaded GGP films were prepared by cross-linking the molecules in presence of epichlorhydrin and two different percentages of KA were employed. Film physical and tensile properties were systematically evaluated and optimized. Water vapour permeability (WVP) and tensile strength of final film GGPFK10 were recorded at 0.741 ± 0.09gmm-1kPa-1h-1 and 19.23 MPa. KA release from GGP matrix followed controlled diffusion process. MIC of GGP was 130 µg/mL and zone of inhibition of GGPFK10 was confirmed at 16.1 mm. SEM experiments disclosed the absence of pili-like structures with squeezed and elongated cellular morphology in dead planktonic cells. Disruption of biofilms was experimented in detail by CV assay, fluorescent, light microscopic and SEM studies. The film showed excellent cell-viability on human adult dermal fibroblast (HADF)cell-line. Overall, the biosafe film would be a potent antibacterial device for treating infections against E.coli biofilms and planktonic cells.
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Escherichia coli , Propionatos , Adulto , Antibacterianos/química , Antibacterianos/farmacología , Biopelículas , Galactanos/química , Galactanos/farmacología , Humanos , Mananos/química , Mananos/farmacología , Plancton , Gomas de Plantas/química , Gomas de Plantas/farmacología , Pironas , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
Partially hydrolyzed guar gum (PHGG) is a soluble dietary fiber derived through controlled enzymatic hydrolysis of guar gum, a highly viscous galactomannan derived from the seeds of Cyamopsis tetragonoloba. Here, we examined the therapeutic potential of dietary supplementation with PHGG against sarcopenic obesity using Db/Db mice. Db/Db mice fed a normal diet alone or a fiber-free diet, or supplemented with a diet containing PHGG (5%), were examined. PHGG increased grip strength and the weight of skeletal muscles. PHGG increased the short-chain fatty acids (SCFAs) concentration in feces and sera. Concerning innate immunity, PHGG decreased the ratio of inflammatory cells, while increasing the ratio of anti-inflammatory cells in the small intestine. The present study demonstrated the preventive effect of PHGG on sarcopenic obesity. Changes in nutrient absorption might be involved through the promotion of an anti-inflammatory shift of innate immunity in the intestine accompanied by an increase in SCFA production by PHGG.
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Sarcopenia , Animales , Galactanos/farmacología , Galactanos/uso terapéutico , Mananos , Ratones , Obesidad/tratamiento farmacológico , Gomas de Plantas/farmacología , Gomas de Plantas/uso terapéutico , Sarcopenia/tratamiento farmacológico , Sarcopenia/prevención & controlRESUMEN
Natural polymers are an efficient class of eco-friendly and biodegradable polymers, because they are readily available, come from natural sources, inexpensive and can be chemically modified with the correct reagents. Guar gum (GG) is a natural polymer with great potential to be used in pharmaceutical formulations due to its unique composition and lack of toxicity. GG can be designed to suit the needs of the biological and medical engineering sectors. In the development of innovative drug delivery systems, GG is commonly utilized as a rate-controlling excipient. In this review, different properties of GG including chemical composition, extraction methods and its usefulness in diabetes, cholesterol lowering, weight control, tablet formulations as well as its food application were discussed. The other purpose of this study is to evaluate potential use of GG and its derivatives for advanced nanomedicine such as drug delivery, tissue engineering and nanosensing. It should be noted that some applicable patents in medical area have also been included in the rest of this survey to extend knowledge about guar gum and its polymeric nature.
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Cyamopsis/química , Galactanos/química , Galactanos/farmacología , Mananos/química , Mananos/farmacología , Gomas de Plantas/química , Gomas de Plantas/farmacología , Animales , Humanos , Nanomedicina/métodosRESUMEN
Osteoarthritis is the most common cause of disability and aging is the major risk factor. One of the low-risk herbal medicines for reducing pain and inflammation in persian medicine is Pistacia atlantica gum. A triple-blind placebo-controlled clinical trial was performed in order to evaluate the efficacy of P. atlantica gum ointment on knee osteoarthritis in elderly people. A total of 60 patients were randomly allocated to two groups. The intervention group used 4% P. atlantica ointment and the control group used placebo, topically applied three times a day, for 8 weeks. The patients were evaluated before and after the intervention using the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) scale. There were significant differences (p < .001) between two the groups in terms of joint stiffness and pain as well as function. Within groups, regarding the effect size (EF) in joint stiffness was 2.82 and 0.74 in the Pistacia and placebo groups, respectively. Regarding pain, the effect size of the intervention and placebo groups were 3.90 and 0.99, respectively. In addition, regarding function, the effect size of the intervention and the placebo groups were 4.33 and 1.17, respectively. Therefore, it seems that topical P. atlantica is significantly better than the placebo in the old patients with knee osteoarthritis.
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Osteoartritis de la Rodilla , Pistacia , Gomas de Plantas/farmacología , Anciano , Método Doble Ciego , Humanos , Medicina Tradicional , Osteoartritis de la Rodilla/tratamiento farmacológico , Dimensión del Dolor , Pistacia/química , Resultado del TratamientoRESUMEN
Polysaccharides can form interfacial complexes with proteins to form emulsions with enhanced stability. We assessed the effect of adding gum guar or gum arabic to egg yolk/fish oil emulsions. The emulsions were produced using simple or high-pressure homogenization, stored for up to 10 days at 45 °C, and characterized for their particle size and distribution, viscosity, encapsulation efficiency, oxidative stability, and cytotoxicity. Emulsions containing gum guar and/or triglycerides had the highest viscosity. There was no significant difference in the encapsulation efficiency of emulsions regardless of the polysaccharide used. However, emulsions containing gum arabic displayed a bridging flocculation effect, resulting in less stability over time compared to those using gum guar. Emulsions produced using high-pressure homogenization displayed a narrower size distribution and higher stability. The formation of peroxides and propanal was lower in emulsions containing gum guar and was attributed to the surface oil. No significant toxicity toward Caco-2 cells was found from the emulsions over time. On the other hand, after 10 days of storage, nonencapsulated fish oil reduced the cell viability to about 80%. The results showed that gum guar can increase the particle stability of egg yolk/fish oil emulsions and decrease the oxidation rate of omega-3 fatty acids.