Asunto(s)
Carcinoma in Situ , Inmunoterapia/efectos adversos , Infecciones por Mycobacterium , Mycobacterium bovis , Enfermedades del Pene , Neoplasias Uretrales , Anciano , Carcinoma in Situ/microbiología , Carcinoma in Situ/terapia , Humanos , Masculino , Infecciones por Mycobacterium/etiología , Infecciones por Mycobacterium/microbiología , Infecciones por Mycobacterium/patología , Enfermedades del Pene/etiología , Enfermedades del Pene/microbiología , Enfermedades del Pene/patología , Neoplasias Uretrales/microbiología , Neoplasias Uretrales/patología , Neoplasias Uretrales/terapiaRESUMEN
BACKGROUND: Urinary tract infections (UTI) are believed to be common in dogs with transitional cell carcinoma (TCC), but incidence and contributing factors have not been reported. OBJECTIVES: To determine the frequency and bacterial agents associated with UTI in dogs with TCC and define contributing factors. ANIMALS: Eighty-five dogs with a history of urogenital TCC undergoing treatment with chemotherapy that had at least 1 urine culture performed. METHODS: Medical records and culture results were retrospectively reviewed and ultrasound images were reviewed when available. Clinical factors were evaluated statistically for association with positive culture. RESULTS: Fifty-five percent (47/85) of dogs had at least 1 positive culture during the course of treatment. Female dogs (80%, 40/50) were more likely than male dogs (29%, 10/35) to have at least 1 positive culture. Ultrasound examination determined that female dogs were more likely to have urethral (74%, 31/42) or trigonal tumor involvement (71%, 30/42) compared to male dogs (32%, 9/28 and 43%, 12/28, respectively). The most commonly isolated organisms were Staphylococcus spp. (23.9%, 29/121) and Escherichia coli (19.8%, 24/121). Dogs with urethral involvement of TCC were significantly more likely to have at least 1 positive culture than dogs without urethral involvement (75%, 30/40 versus 30%, 9/30). CONCLUSIONS: Urinary tract infection is common in dogs with TCC highlighting the importance of regular monitoring for bacterial cystitis in dogs with TCC. In addition, clinical factors such as tumor location and sex may be predictive of positive culture and can help clinicians assess the risk of UTI.
Asunto(s)
Carcinoma de Células Transicionales/veterinaria , Enfermedades de los Perros/microbiología , Infecciones Urinarias/veterinaria , Neoplasias Urológicas/veterinaria , Animales , Antibacterianos/uso terapéutico , Carcinoma de Células Transicionales/complicaciones , Carcinoma de Células Transicionales/microbiología , Enfermedades de los Perros/tratamiento farmacológico , Perros , Infecciones por Escherichia coli/complicaciones , Infecciones por Escherichia coli/tratamiento farmacológico , Infecciones por Escherichia coli/veterinaria , Femenino , Masculino , Pruebas de Sensibilidad Microbiana/veterinaria , Factores Sexuales , Infecciones Estafilocócicas/complicaciones , Infecciones Estafilocócicas/microbiología , Infecciones Estafilocócicas/veterinaria , Neoplasias Uretrales/complicaciones , Neoplasias Uretrales/microbiología , Neoplasias Uretrales/veterinaria , Infecciones Urinarias/complicaciones , Infecciones Urinarias/tratamiento farmacológico , Infecciones Urinarias/microbiología , Neoplasias Urológicas/complicaciones , Neoplasias Urológicas/microbiologíaRESUMEN
Rhinosporidiosis is a very common disease endemic in India, Ceylon, East Africa and part of America. Extremely rare in Europe. Nose, naso-pharynx, nasal sinuses, larynx are the common sites of affection, but urethral lesion is very rare. Reports from India so far as our knowledge goes are not available. Symmers may be the only case report available so far. For the rarity of site of affection, the case is reported.
Asunto(s)
Pólipos/patología , Rinosporidiosis/patología , Neoplasias Uretrales/patología , Humanos , Masculino , Persona de Mediana Edad , Pólipos/etiología , Pólipos/microbiología , Rinosporidiosis/complicaciones , Rinosporidiosis/microbiología , Neoplasias Uretrales/etiología , Neoplasias Uretrales/microbiologíaRESUMEN
We report a primary non-Hodgkin's B-cell lymphoma of the urethra in a 78-year-old female. Serum antibodies for Epstein-Barr virus (EBV) were negative, but there was a 40-fold increase in antibodies to EBV-associated nuclear antigen. Using PCR and in situ hybridization techniques, EBV genome was found in the tumour cell nuclei.
Asunto(s)
Infecciones por Herpesviridae/microbiología , Herpesvirus Humano 4/aislamiento & purificación , Linfoma de Células B/microbiología , Infecciones Tumorales por Virus/microbiología , Neoplasias Uretrales/microbiología , Anciano , Anticuerpos Antivirales/sangre , Antígenos Virales/inmunología , Proteínas de Unión al ADN/inmunología , Electroforesis en Gel de Agar , Antígenos Nucleares del Virus de Epstein-Barr , Femenino , Técnica del Anticuerpo Fluorescente , Genoma Viral , Infecciones por Herpesviridae/patología , Herpesvirus Humano 4/genética , Herpesvirus Humano 4/inmunología , Humanos , Inmunohistoquímica , Hibridación in Situ/métodos , Linfoma de Células B/inmunología , Linfoma de Células B/patología , Fenotipo , Reacción en Cadena de la Polimerasa , Células Tumorales Cultivadas , Infecciones Tumorales por Virus/patología , Neoplasias Uretrales/inmunología , Neoplasias Uretrales/patologíaRESUMEN
A rare case of urethral flat wart caused by human papillomavirus (HPV) type 16 was reported. A 34-year-old male consulted our department with a complaint of urethral bleeding. A pair of symmetrical white flat warts combined with ulcer at the urethral meatus were found. Microscopic examination of the specimen demonstrated koilocytosis of the epithelium. In immunohistochemical study it was positive for HPV and HPV type 16 was detected by polymerase chain reaction.
Asunto(s)
Condiloma Acuminado/microbiología , Papillomaviridae/aislamiento & purificación , Neoplasias del Pene/microbiología , Neoplasias Uretrales/microbiología , Adulto , Humanos , Masculino , Reacción en Cadena de la PolimerasaRESUMEN
Human papillomaviruses (HPV), especially genotypes 16 and 18, are probable effectors of human urogenital malignancies. Although the male urethra is a proposed reservoir of HPV transmission, the association between HPV and squamous cell carcinoma (SCC) of the male urethra has not been studied. The highly sensitive technique of polymerase chain reaction with type-specific HPV 16 and 18 primers and general primers, including nine other genotypes was used to survey a series of SCC of the male urethra for the prevalence of an association with HPV. Archival surgical specimens from 14 patients were analyzed, and primary, recurrent, and metastatic lesions from 4 (29%) patients contained HPV 16 DNA. No other HPV genotype (6b, 11, 13, 18, 30, 31, 33, 35, 45, 51) was detected. Complete concordance for the presence of HPV in primary and recurrent or metastatic disease was demonstrated. These findings strongly suggest that HPV type 16 is associated with a substantial subset of SCCs of the male urethra. Analysis of clinical data revealed that HPV-positive tumors had a significant predilection for location in the pendulous urethra versus the bulbar urethra. Survival data analysis showed that the presence of HPV more closely correlated with prolonged survival than did tumor location. The presence or absence of HPV 16 DNA defines two subsets of SCC of the male urethra which differ in the site of occurrence and, possibly, progression.
Asunto(s)
Carcinoma de Células Escamosas/microbiología , Papillomaviridae/patogenicidad , Infecciones Tumorales por Virus/diagnóstico , Neoplasias Uretrales/microbiología , Secuencia de Bases , ADN Viral/análisis , Humanos , Masculino , Datos de Secuencia Molecular , Oligodesoxirribonucleótidos/química , Papillomaviridae/clasificación , Reacción en Cadena de la Polimerasa , Estudios Retrospectivos , Análisis de Supervivencia , Infecciones Tumorales por Virus/microbiologíaRESUMEN
We report on an HIV-positive male patient with a giant condyloma acuminatum at the base of penis coexisting with simple intraurethral condylomas and no crown lesions. The association of both processes indicate the likelihood of a common viral etiology. The presentation of this florid picture in a terminal patient with AIDS indicates that urologists are likely to find an increased incidence of this condition in the forthcoming years.
Asunto(s)
Carcinoma Papilar , Condiloma Acuminado , Neoplasias Primarias Múltiples , Neoplasias del Pene , Neoplasias Uretrales , Adulto , Carcinoma Papilar/complicaciones , Carcinoma Papilar/microbiología , Condiloma Acuminado/complicaciones , Condiloma Acuminado/microbiología , Infecciones por VIH/complicaciones , Humanos , Masculino , Papillomaviridae/patogenicidad , Neoplasias del Pene/complicaciones , Neoplasias del Pene/microbiología , Infecciones Tumorales por Virus/complicaciones , Neoplasias Uretrales/complicaciones , Neoplasias Uretrales/microbiologíaRESUMEN
A case of urethral condyloma acuminatum is reported. A 77-year-old man was admitted to Konan Hospital to undergo surgery for benign prostatic hyperplasia. By urethrography and urethroscopy, a circumferential papillary lesion was disclosed in the posterior urethra extending from the membraneous urethra. Transurethral resection was performed on July 19, 1989. Pathohistological diagnosis was condyloma acuminatum/condyloma acuminatum in which the antigen of papilloma virus was detected by PAP stain.
Asunto(s)
Condiloma Acuminado/cirugía , Neoplasias Uretrales/cirugía , Anciano , Condiloma Acuminado/microbiología , Humanos , Masculino , Papillomaviridae/aislamiento & purificación , Neoplasias Uretrales/microbiologíaRESUMEN
It has been reported that deoxyribonuclease (DNAse) treatment does not destroy viral DNA, but it does digest native nuclear DNA. To determine what effect, if any, papillomavirus infection has on DNA ploidy values of genitourinary condylomas, DNA was measured with and without DNAse exposure in seven urethral condylomas, shown by prior in situ hybridization to contain abundant human papillomavirus types 6 and 11. Normal human skin was used as a negative control. Consecutive paraffin-embedded tissue sections were stained according to Feulgen before and after DNAse treatment. The DNA was measured by image analysis. In control tissue, DNAse obliterated DNA, and the Feulgen reaction was negative. In six of seven condylomas the DNA content was reduced, but a measurable Feulgen reaction was still present in isolated cells. In the seventh case there were no significant changes in the histograms. This observation strongly suggests that the presence of human papillomavirus has a significant effect on measurements of DNA ploidy in genital condylomas and, by implication, possibly also in other tissues containing the virus. Possible mechanisms are discussed.
Asunto(s)
Condiloma Acuminado/genética , ADN de Neoplasias/análisis , ADN Viral/análisis , Papillomaviridae/genética , Neoplasias Uretrales/genética , Adulto , Condiloma Acuminado/microbiología , Humanos , Masculino , Hibridación de Ácido Nucleico , Ploidias , Neoplasias Uretrales/microbiologíaRESUMEN
Of 4 patients who underwent cystourethroscopy, biopsy and laser excision of suspected urethral condylomata acuminata 3 had coexistent grade I papillary transitional cell carcinoma of the urethra. Human papillomavirus type 6 messenger ribonucleic acid was demonstrated within biopsy specimens using tritium-labeled single-stranded antisense ribonucleic acid probes. Compared to condylomata the papillary transitional epithelium expressed less viral message, which might be expected in an epithelium that does not show full squamous epithelial or koilocytotic differentiation. Among these patients there was 1 papillary transitional lesion in the bladder that, although histologically similar, did not express human papillomavirus message, suggesting differential susceptibility of epithelium between the bladder and urethra. The finding of active human papillomavirus transcription within the urethral papillary transitional lesions raises the possibility of an active role for the virus in the pathogenesis of these lesions. These findings broaden the spectrum of epithelial types reported to support human papillomaviruses and provides impetus for a wider search for these viruses in other transitional cell neoplasms.
Asunto(s)
Carcinoma de Células Transicionales/microbiología , Papillomaviridae/aislamiento & purificación , Neoplasias Uretrales/microbiología , Adulto , Carcinoma de Células Transicionales/patología , Condiloma Acuminado/microbiología , Condiloma Acuminado/patología , Humanos , Masculino , Neoplasias Primarias Múltiples/microbiología , Neoplasias Primarias Múltiples/patología , Neoplasias Uretrales/patologíaRESUMEN
Human papillomavirus (HPV) detection was performed using the polymerase chain reaction technique on urine samples from 17 male patients with condylomata acuminata in the meatus urethrae. Urine samples from 14 male laboratory volunteers were analyzed as controls. The DNA was extracted and purified from urine sediments, centrifuged at 1,800 and 100,000 x g, and subjected to 40 cycles of amplification with HPV 6 and HPV 11 type-specific anticontamination primers and the heat-stable Taq DNA polymerase. HPV was detected in the urine of 15 (88%) patients. In all positive patients the urine sediments of both the 1,800 and 100,000 x g centrifugation steps contained HPV DNA. Eight patients were found to be positive for HPV 6 DNA, six were positive for HPV 11 DNA, and one was positive for both HPV 6 and HPV 11 DNA. None of the males in the control group was positive for either HPV 6 or HPV 11 DNA. The results demonstrate that HPV can be transported by the urine, probably in exfoliated HPV-infected cells. A similar mechanism may occur during ejaculation, allowing sexual transmission of HPV viruses harbored in the cells of the male genital tract.
Asunto(s)
Condiloma Acuminado/orina , ADN Viral/orina , Papillomaviridae/aislamiento & purificación , Neoplasias Uretrales/orina , Secuencia de Bases , Southern Blotting , Condiloma Acuminado/microbiología , Sondas de ADN de HPV , ADN Viral/genética , Electroforesis en Gel de Agar , Amplificación de Genes , Humanos , Masculino , Datos de Secuencia Molecular , Papillomaviridae/genética , Neoplasias Uretrales/microbiologíaRESUMEN
The authors report a case of primary urethral carcinoma which seems to be linked to human papillomavirus (HPV) infection. Southern blot hybridization of phosphorus 32 (32P)-labeled DNA extracted from the cancer tissue gave a positive reaction with HPV-6. Specimens of the tumor material subjected to in situ hybridization with 3H-labeled HPV-6 DNA showed numerous tumor cell clusters with clearly labeled nuclei. Using immunoperoxidase staining the authors found papillomavirus structural antigen within cell nuclei of the tumor tissue.
Asunto(s)
Carcinoma de Células Escamosas/microbiología , ADN Viral/análisis , Hibridación de Ácido Nucleico , Papillomaviridae/genética , Infecciones Tumorales por Virus/diagnóstico , Neoplasias Uretrales/microbiología , Antígenos Virales/análisis , Carcinoma de Células Escamosas/etiología , Humanos , Técnicas para Inmunoenzimas , Masculino , Persona de Mediana Edad , Neoplasias Uretrales/etiologíaRESUMEN
An in situ hybridization technique was applied under stringent conditions to paraffin sections of urethral condylomata from male patients to determine the presence of DNA sequences of human papillomavirus (HPV) types 6, 11, 16, and 18. The material consisted of 15 classical condylomata acuminata, two flat condylomata, and five recurrent lesions. HPV DNA sequences could be identified in all 15 condylomata acuminata; in 13 lesions, two types of viral DNA were observed (types 6 and 11 in 12, types 6 and 18 in one). In the remaining two condylomata acuminata, only HPV type 11 was present. One of the two flat condylomata was negative with all the probes, and one was borderline-positive for HPV 6. Four of five recurrent lesions contained the same types of viral DNA as the primary lesions, albeit with slight differences in the intensity of viral expression. One lesion was negative with all probes. We conclude that urethral condylomata in males contain the same types of HPV as seen in other anogenital lesions of both sexes and that infection with two viral types is common. In situ hybridization with HPV DNA probes is applicable to archival material and therefore may prove to be of value in future epidemiologic studies comparing lesions in sexual partners. The determination of viral type may have therapeutic implications.
Asunto(s)
Condiloma Acuminado/microbiología , Papillomaviridae/clasificación , Neoplasias Uretrales/microbiología , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Niño , ADN , Humanos , Masculino , Persona de Mediana Edad , Hibridación de Ácido NucleicoRESUMEN
Twenty-five squamous papillomas of the male urethra were examined for the presence of human papillomavirus (HPV) whole (structural) virion antigens by a peroxidase-antiperoxidase technique having immunospecificity against genus-specific (common) antigens of the papillomavirus genus. Eleven of 25 (44 per cent) lesions were positive for HPV. The positive reaction was invariably intranuclear in the superficial epithelium. Evidence suggests that these papillomas arise by HPV infection of metaplastic squamous epithelium and not transitional epithelium. This study shows that at least some squamous papillomas of the male urethra are associated with HPV infections and suggest that this lesion may play an important role in venereally transmitted diseases such as cervical dysplasia and condylomas of the vulva and anus.
Asunto(s)
Antígenos Virales/análisis , Papiloma/inmunología , Papillomaviridae/inmunología , Neoplasias Uretrales/inmunología , Adolescente , Adulto , Humanos , Técnicas para Inmunoenzimas , Masculino , Persona de Mediana Edad , Papiloma/microbiología , Neoplasias Uretrales/microbiologíaRESUMEN
Simultaneous detection assays on the core structures derived from the cerebrospinal fluid samples of patients with various types of central nervous system tumors have demonstrated the feasibility of this technique in detecting some of the diagnostic features of RNA tumor viruses. Similar assays done on urine samples from patients iwth various types of tumors in their genitourinary tracts have shown that of the 18 such samples from tumor patients, 15 or 83% were found to be positive. The control samples consisted of three from patients with benign prostatic hypertrophy and four from normal persons. None of these gave a positive reaction. [3H]DNA probes synthesized from the core structures from them hybridized readily to their corresponding polysomal RNAs but no to control tissues. The densities of particles from these samples have been found to be 1.168 g/ml for bladder carcinoma and 1.165 for prostatic carcinoma, the same densities as those found RNA tumor viruses.