Sensitive visual detection of norfloxacin in water by smartphone assisted colorimetric method based on peroxidase-like active cobalt-doped Fe3O4 nanozyme.

Norfloxacin is widely used owing to its strong bactericidal effect on Gram-negative bacteria. However, the residual norfloxacin in the environment can be biomagnified via food chain and may damage the human liver and delay the bone development of minors. Present work described a reliable and sensitive smartphone colorimetric sensing system based on cobalt-doped Fe3O4 magnetic nanoparticles (Co-Fe3O4 MNPs) for the visual detection of norfloxacin. Compared with Fe3O4, Co-Fe3O4 MNPs earned more remarkably peroxidase-like activity and TMB (colorless) was rapidly oxidized to oxTMB (blue) with the presence of H2O2. Interestingly, the addition of low concentration of norfloxacin can accelerate the color reaction process of TMB, and blue deepening of the solution can be observed with the naked eye. However, after adding high concentration of norfloxacin, the activity of nanozyme was inhibited, resulting in the gradual fading of the solution. Based on this principle, a colorimetric sensor integrated with smartphone RGB mode was established. The visual sensor exhibited good linearity for norfloxacin monitoring in the range of 0.13-2.51 µmol/L and 17.5-100 µmol/L. The limit of visual detection was 0.08 µmol/L. In the actual water sample analysis, the spiked recoveries of norfloxacin were over the range of 95.7%-104.7 %. These results demonstrated that the visual sensor was a convenient and fast method for the efficient and accurate detection of norfloxacin in water, which may have broad application prospect.

Novel Br-doped Bi2WO6 photocatalyst for high-efficiency removal of norfloxacin: mechanism and photocatalytic activity.

In this work, the bismuth tungstate (Bi2WO6) photocatalyst was successfully prepared, and the pure Bi2WO6 was modified with Br (Br-Bi2WO6). The effects of different experimental conditions on the degradation of norfloxacin (NOR) solution under visible light were investigated. The Br-Bi2WO6 photocatalyst was characterized by FTIR, XRD, SEM, BET, XPS, DRS, EIS, and EPR. The results show that the Br-modified Bi2WO6 photocatalyst can effectively improve the photocatalytic performance. The best photocatalytic performance of Br-Bi2WO6 was observed when the doping amount of Br was 3%. The degradation percentage of norfloxacin can reach 94.67%. The presence of anions and cations (Cl-, SO42-, Ag+, and Cu2+) in the solution significantly inhibited the photocatalytic activity of 3%Br-Bi2WO6. The photocatalytic degradation of norfloxacin by 3%Br-Bi2WO6 was not greatly affected in the presence of HCO3- and NO3-. The characterization analysis showed that Br was successfully doped on the Bi2WO6 photocatalyst, and the original structure of Bi2WO6 was not destroyed by the addition of Br. Br doping increased the specific surface area of Bi2WO6 and decreased the band gap of Bi2WO6 resulting in a broader visible light absorption range. In addition, Br doping promoted the migration rate of photogenerated electron-hole pairs. The ·O2- and h+ played a major role in the photodegradation of norfloxacin, and the mechanism of photocatalytic degradation of norfloxacin by Br-Bi2WO6 was proposed.

A ratiometric fluorescence sensor with different responsive modes based on carbon dots-embedded Tb-MOFs for the determination of norfloxacin and levofloxacin.

Norfloxacin (NOR) and levofloxacin (LEV) are the two most frequently used fluoroquinolones (FQs) in clinic. Their residues seriously endanger the ecosystem and human health. Due to their similarity in structure and properties, it is urgent to develop an efficient and sensitive strategy for detection and differentiation. Herein, we synthesized a novel ratiometric fluorescent sensor for the first time by combining N, S co-doped carbon dots (CDs) and the precursors of Tb-MOFs through a facile one-pot method. The introduction of CDs effectively facilitated the energy transfer between Tb3+ and FQs, overcoming the limitation that single Tb-MOFs could not identify similar antibiotics. Specifically, the presence of NOR resulted in reverse signal response through the inner filter effect and antenna effect. The synergistic effect of these two mechanisms contributed to achieving signal amplification accompanied by a distinguishable color transition. The limit of detection (LOD) was 0.036 µM. Different from NOR, the addition of LEV reduced the electron density of the system, weakened the coordination ability of Tb3+ with LEV, and induced a single signal response with Tb3+ fluorescence intensity as a reference signal (LOD = 0.383 µM). Furthermore, the method proved to be rapid and visual, allowing for the straightforward analysis of FQs residues in water, food matrices, and biological samples with satisfactory precision. By integrating N, S-CDs@Tb-MOFs with flexible substrates, the paper-based sensor facilitated the visual quantitative determination of FQs by reading RGB values. The developed sensor presents a promising strategy for the identification and real-time monitoring of antibiotics.

Design, synthesis and evaluation of novel norfloxacin analogs as potent anticancer and antioxidant agents.

Aim: To synthesize a novel series of norfloxacin analogs and to evaluate biological activity.Methodology: Novel norfloxacin analogs were synthesized and characterized by NMR and mass spectrometry. Antiproliferative and antioxidant properties were studied.Results: Compound 2f was the most potent against HeLa cell-line with 100% inhibition of cell viability IC50 = 3.1 ± 0.2 µM. All compounds exhibit moderate to excellent antioxidant properties. Docking study demonstrates higher binding affinity of compounds with respective anticancer (B-cell lymphoma-2) and (tyrosinase) antioxidant targets. In silico absorption, distribution, metabolism and excretion profile of compounds proves all synthesized compounds follow Lipinski's rule of drug likeness, non toxic and possess passive gastrointestinal absorption.Conclusion: The biological profile suggest that the synthesized norfloxacin analogs can be a novel scaffold for future anticancer drug development.

[Box: see text].

Bi2MoO6/MWCNTs-COOH/GCE electrochemical probe for ultrasensitively and selectively detecting antibiotic norfloxacin.

Antibiotics have emerged as a class of contaminants of concern globally, raising widespread worry and unease, primarily because of their inappropriately use and endless migration in food chains. Electrochemical ultrasensitive and selective determination of norfloxacin (NOR) using bismuth-based bimetallic salt bismuth molybdate (Bi2MoO6) and carboxylated multi-walled carbon nanotube (MWCNTs-COOH) hybrid electrode as sensing platforms is presented hereby. The electrocatalytic ability of the electrodes was verified to be enhanced by the synergy effect of both Bi2MoO6 and MWCNTs-COOH using cyclic voltammetry and electrochemical impedance spectroscopy. Linear scan voltammetry was applied to detect NOR. The oxidation peak current was linearly correlated with NOR concentration of 0.03-10 µM and the low limit detection of the optimized method was 6.7 nM (S/N = 3). In addition, it was verified that Bi2MoO6/MWCNTs-COOH/GCE probe had good stability, reproducibility, repeatability and selectivity. The spiked recovery method for NOR in milk and lake water samples showed recoveries of 94.8 - 96.9% and 90.2 - 98.3%, respectively, which can be used to determine NOR in real samples with high sensitivity. The preparation of Bi2MoO6/MWCNTs-COOH/GCE provides a new prototype for probing NOR detection at nanomolar concentration and safeguarding antibiotic contamination in environmental and food chains.

Nanoplastics composite norfloxacin induced changes in conformation and function of lysozyme and differential effects of co-exposure contamination.

The concurrent environmental contamination by nanoplastics (NPs) and norfloxacin (NOR) is a burgeoning concern, with significant accumulations in various ecosystems and potential ingress into the human body via the food chain, posing threats to both public health and ecological balance. Despite the gravity of the situation, studies on the co-exposure contamination effects of these substances are limited. Moreover, the response mechanisms of key functional proteins to these pollutants are yet to be fully elucidated. In this work, we conducted a comprehensive assessment of the interaction mechanisms of NPs and NOR with lysozyme under both single and co-exposure condition, utilizing dynamic light scattering, ζ-potential measurements, multi-spectroscopy methods, enzyme activity assays and molecular docking, to obtain a relationship between the compound effects of NPs and NOR. Our results indicate that NPs adsorb NOR on their surface, forming more stable aggregates. These aggregates influence the conformation, secondary structure (α-Helix ratio decreased by 3.1 %) and amino acid residue microenvironment of lysozyme. And changes in structure affect the activity of lysozyme (reduced by 39.9 %) with the influence of composited pollutants exerting stronger changes. Molecular simulation indicated the key residues Asp 52 for protein function located near the docking site, suggesting pollutants preferentially binds to the active center of lysozyme. Through this study, we have found the effect of increased toxicity on lysozyme under the compounded conditions of NPs and NOR, confirming that the increased molecular toxicity of NPs and NOR is predominantly realized through the increase in particle size and stability of the aggregates under weak interactions, as well as induction of protein structural looseness. This study proposes a molecular perspective on the differential effects and mechanisms of NPs-NOR composite pollution, providing new insights into the assessment of in vitro responses to composite pollutant exposure.

Combining Vis-NIR and NIR hyperspectral imaging techniques with a data fusion strategy for prediction of norfloxacin residues in mutton.

Norfloxacin is an antibacterial compound that belongs to the fluoroquinolone family. Currently, hyperspectral imaging (HSI) for the detection of antibiotic residues focuses mostly on individual systems. Attempts to integrate different HSI systems with complementary spectral ranges are still lacking. This study investigates the feasibility of applying data fusion strategies with two HSI techniques (Visible near-infrared and near-infrared) in combination to predict norfloxacin residue levels in mutton. Spectral data from the two spectral techniques were analyzed using partial least squares regression (PLSR), support vector regression (SVR) and stochastic configuration networks (SCN), respectively, and the two data fusion strategies were fused at the data level (low-level fusion) and feature level (middle-level fusion, mid-level fusion). The results indicated that the modeling performance of the two fused datasets was better than that of the individual systems. Mid-level fusion data achieved the best model based on uninformative variable elimination (UVE) combined with SCN, in which the determination coefficient of prediction set (R2p) of 0.9312, (root mean square error of prediction set) RMSEP of 0.3316 and residual prediction deviation (RPD) of 2.7434, in comparison with all others. Therefore, two HSI systems with complementary spectral ranges, combined with data fusion strategies and feature selection, could be used synergistically to improve the detection of norfloxacin residues. This study may provide a valuable reference for the non-destructive detection of antibiotic residues in meat.

Micropollutants (ciprofloxacin and norfloxacin) remediation from wastewater through laccase derived from spent mushroom waste: Fate, toxicity, and degradation.

Fluoroquinolone antibiotics frequently found in environmental matrices (wastewater treatment plants, hospital wastewater, industrial wastewater and surface wastewater) causes potential threat to the environment. Enzymatic treatment for degradation of antibiotics from environmental matrices is a green and sustainable approach. Focusing on this, this study aimed to degrade two frequently found fluroquinolone emergent pollutants, ciprofloxacin and norfloxacin from wastewater. The trinuclear cluster of copper ions present in laccase has the ability to effectively remove organic micropollutants (OMPs). The uniqueness of this study is that it utilizes laccase enzyme extracted from spent mushroom waste (SMW) of P. florida for degradation of ciprofloxacin and norfloxacin and to achieve highest degradation efficiency various parameters were tweaked such as pH (3-6), temperature (30 °C and 50 °C), and ABTS (0.05, 0.6, and 1 mM) concentration. The results showed that the most effective degradation of ciprofloxacin (86.12-75.94%) and norfloxacin (83.27-65.94%) was achieved in 3 h at pH 4.5, temperature 30 °C, and 2,2'-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 0.05 mM concentration. Nevertheless, achieving degradation at 50 °C for both antibiotics, indicates thermostability nature of laccase (P. florida). Further, the fate of transformed products obtained from laccase mediated degradation was confirmed by liquid chromatography (LC-MS). Both the antibiotics undergo decarboxylation, depiperylyzation, dealkylation and defluorination as a result of laccase-mediated bond breakage. Anti-microbial activity of the biodegraded products was monitored by residual anti-bacterial toxicity test (E. coli and Staphylococcus aureus). The biodegraded products were found to be non-toxic and resulted in the growth of E. coli and Staphylococcus aureus, as determined by the agar-diffusion method. Moreover, the storage stability of laccase was determined for 28-day duration at varying pH (3-10) and temperature (4-50 °C). The maximum storage stability was obtained at pH 4.5 and temperature 30 °C. Therefore, utilizing SMW for the degradation of OMPs from wastewater not only benefits in degradation but also reuses SMW agro waste, shedding light on agro waste management. Thus, SMW is a one-pot solution for both OMPs biodegradation and circularity in the economy.

Conserved Evolutionary Trajectory Can Be Perturbed to Prevent Resistance Evolution under Norfloxacin Pressure by Forcing Mycobacterium smegmatis on Alternate Evolutionary Paths.

Antibiotic resistance is a pressing health issue, with the emergence of resistance in bacteria outcompeting the discovery of novel drug candidates. While many studies have used Adaptive Laboratory Evolution (ALE) to understand the determinants of resistance, the influence of the drug dosing profile on the evolutionary trajectory remains understudied. In this study, we employed ALE on Mycobacterium smegmatis exposed to various concentrations of Norfloxacin using both cyclic constant and stepwise increasing drug dosages to examine their impact on the resistance mechanisms selected. Mutations in an efflux pump regulator, LfrR, were found in all of the evolved populations irrespective of the drug profile and population bottleneck, indicating a conserved efflux-based resistance mechanism. This mutation appeared early in the evolutionary trajectory, providing low-level resistance when present alone, with a further increase in resistance resulting from successive accumulation of other mutations. Notably, drug target mutations, similar to those observed in clinical isolates, were only seen above a threshold of greater than 4× the minimum inhibitory concentration (MIC). A combination of three mutations in the genes, lfrR, MSMEG_1959, and MSMEG_5045, was conserved across multiple lineages, leading to high-level resistance and preceding the appearance of drug target mutations. Interestingly, in populations evolved from parental strains lacking the lfrA efflux pump, the primary target of the lfrR regulator, no lfrR gene mutations are selected. Furthermore, evolutional trajectories originating from the ΔlfrA strain displayed early arrest in some lineages and the absence of target gene mutations in those that evolved, albeit delayed. Thus, blocking or inhibiting the expression of efflux pumps can arrest or delay the fixation of drug target mutations, potentially limiting the maximum attainable resistance levels.

Simultaneous photoactivation of a fluoroquinolone antibiotic and nitric oxide with fluorescence reporting.

The achievement of smart pharmaceuticals whose bioactivity can be spatiotemporally controlled by light stimuli is known as photopharmacology, an emerging area aimed at improving the therapeutic outcome and minimizing side effects. This is especially attractive for antibiotics, for which the inevitable development of multidrug resistance and the dwindling of new clinically approved drugs represent the main drawbacks. Here, we show that nitrosation of the fluoroquinolone norfloxacin (NF), a broad-spectrum antibiotic, leads to the nitrosated bioconjugate NF-NO, which is inactive at the typical minimum inhibitory concentration of NF. Irradiation of NF-NO with visible blue light triggers the simultaneous release of NF and nitric oxide (NO). The photouncaging process is accompanied by the revival of the typical fluorescence emission of NF, quenched in NF-NO, which acts as an optical reporter. This permits the real-time monitoring of the photouncaging process, even within bacteria cells where antibacterial activity is switched on exclusively upon light irradiation. The mechanism of photorelease seems to occur through a two-step hopping electron transfer mediated by the lowest triplet state of NF-NO and the phosphate buffer ions or aminoacids such as tyrosine. Considering the well-known role of NO as an "unconventional" antibacterial, the NF-NO conjugate may represent a potential bimodal antibacterial weapon activatable on demand with high spatio-temporal control.

Nitrogen-doped carbon dots: a novel biosensing platform for selective norfloxacin detection and bioimaging.

Incomplete metabolism and non-biodegradable nature of norfloxacin (NORx) lead to its persistent residues in the environment and food, potentially fostering the emergence of antibiotic resistance and posing a significant threat to public health. Hence, we developed a norfloxacin sensor employing hydrothermally synthesized N-doped carbon dots (N-Ch-CQDs) from chitosan and PEI demonstrated high sensitivity and specificity towards the antibiotic detection. The quantum yield of excitation-dependent emission of N-Ch-CQDs was effectively tuned from 4.6 to 21.5% by varying the concentration of PEI (5-15%). With the enhanced fluorescence in the presence of norfloxacin, N-Ch-CQDs exhibited a linear detection range of 20-1400 nM with a limit of detection (LoD) of 9.3 nM. The high biocompatibility of N-Ch-CQDs was confirmed in the in vitro and in vivo model and showed the environment-friendly nature of the sensor. Detailed study elucidated the formation of strong hydrogen bonds between N-Ch-CQDs and NORx, leading to fluorescence enhancement. The developed sensor's capability to detect NORx was evaluated in water and milk samples. The recovery rate ranged from 98.5% to 103.5%, demonstrating the sensor's practical applicability. Further, the bioimaging potential of N-Ch-CQDs was demonstrated in both the in vitro (L929 cells) and in vivo model (C. elegans). The synergistic influence of the defecation pattern and functioning of intestinal barrier mitigates the translocation of N-Ch-CQDs into the reproductive organ of nematodes. This study revealed the bioimaging and fluorescent sensing ability of N-Ch-CQDs, which holds significant promise for extensive application in the biomedical field.

Norfloxacin affects inorganic nitrogen compound transformation in tailwater containing Corbicula fluminea.

The Asian clam, Corbicula fluminea, commonly used in engineered wetlands receiving tailwater, affects nitrogen compound transformation in water. This study investigates how a commonly observed antibiotic in tailwater, norfloxacin, impact nitrogen compound transformation in tailwater containing C. fluminea. The clam was exposed to artificial tailwater with norfloxacin (0, 0.2, 20, and 2000 µg/L) for 15 days. Water properties, C. fluminea ecotoxicity responses, microorganism composition and nitrification- or denitrification-related enzyme activities were measured. Results revealed norfloxacin-induced increases and reductions in tailwater NH4+ and NO2- concentrations, respectively, along with antioxidant system inhibition, organ histopathological damage and disruption of water filtering and digestion system. Microorganism composition, especially biodiversity indices, varied with medium (clam organs and exposure water) and norfloxacin concentrations. Norfloxacin reduced NO2- content by lowering the ratio between microbial nitrifying enzyme (decreased hydroxylamine oxidoreductase and nitrite oxidoreductase activity) and denitrifying enzyme (increased nitrate reductase and nitrite reductase activity) in tailwater. Elevated NH4+ content resulted from upregulated ammonification and inhibited nitrification of microorganisms in tailwater, as well as increased ammonia emission from C. fluminea due to organ damage and metabolic disruption of the digestion system. Overall, this study offers insights into using benthic organisms to treat tailwater with antibiotic residues, especially regarding nitrogen treatment.

Enhanced solar light-driven photocatalysis of norfloxacin using Fe-doped TiO2: RSM optimization, DFT simulations, and toxicity study.

This study investigates the photocatalytic degradation of norfloxacin (NFX) utilizing Fe-doped TiO2 nanocomposite under natural sunlight. TiO2-based photocatalysts were synthesized using chemical precipitation varying Fe-dopant concentration and characterized in detail. Theoretical modelling, centred on density functional theory (DFT), elucidated that Fe ions within the TiO2 lattice are effectively confined, thereby narrowing the wide band gap of TiO2. The findings strongly support that Fe3+ ions augmented the photocatalytic activity of TiO2 by facilitating an intermediate interfacial route for electron and hole transfer, particularly up to an optimal dopant concentration of 1.5 M%. Subsequently, a three-level Box-Behnken design (BBD) was developed to determine the initial pH, optimal catalyst concentration, and drug dosage. High-performance liquid chromatography-mass spectrometry (HPLC-MS) was employed to identify reaction intermediates, thereby establishing a potential degradation pathway. Notably, sustained recyclability was achieved, with 82% degradation efficiency maintained over five cycles. Additionally, the toxicity of degradation intermediates was evaluated through bacterial and phytotoxicity tests, affirming the environmental safety of treated water. In vitro toxicity of the nanomaterial was also examined, emphasizing its environmental implications. Scavenger experiments revealed that hole and hydroxyl radicals were the primary active species in Fe-TiO2-based photocatalysis. Furthermore, the antibacterial potential of the synthesized catalyst was assessed using Escherichia coli and Staphylococcus aureus to observe their respective antibacterial responses.

Performance and mechanism of sulfadiazine and norfloxacin adsorption from aqueous solution by magnetic coconut shell biochar.

In this study, magnetic coconut shell biochar loaded with spherical Fe3O4 and γ-Fe2O3 particles was successfully synthesized using a chemical coprecipitation method. The magnetic biochar exhibited a good magnetic separability and environmental security. The maximum sulfadiazine (SDZ) and norfloxacin (NOR) removal efficiencies were 94.8% and 92.3% at pH 4 and 25 °C with adsorbent dosage of 2.5 g/L, respectively. When antibiotic concentrations ranged from 5 to 50 mg/L, the theoretical maximum adsorption capacities of SDZ and NOR were 16.7 mg/g and 25.8 mg/g, respectively. The Langmuir isotherm and pseudo-second-order kinetic models could better describe the adsorption process of both antibiotics, implying the monolayer chemical adsorption. The thermodynamic analysis indicated that the adsorption process was spontaneous and endothermic. The ionic strength had no significant effect on the adsorption behavior of either antibiotic. Combined with BET, FTIR, and XPS results, the dominant mechanisms for SDZ and NOR adsorption were pore filling, π-π electron-donor-acceptor interaction, hydrogen bonds and surface complexation. Moreover, Lewis acid-base interaction also contributed to SDZ adsorption.

Label-free liquid crystal-based optical detection of norfloxacin using an aptamer recognition probe in soil and lake water.

Norfloxacin (NOX), a broad spectrum fluoroquinolone (FQ) antibiotic, is commonly detected in environmental residues, potentially contributing to biological drug resistance. In this paper, an aptamer recognition probe has been used to develop a label-free liquid crystal-based biosensor for simple and robust optical detection of NOX in aqueous solutions. Stimuli-receptive liquid crystals (LCs) have been employed to report aptamer-target binding events at the LC-aqueous interface. The homeotropic alignment of LCs at the aqueous-LC interface is due to the self-assembly of the cationic surfactant cetyltrimethylammonium bromide (CTAB). In the presence of the negatively charged NOX aptamer, the ordering changes to planar/tilted. On addition of NOX, the aptamer-NOX binding causes redistribution of CTAB at the LC-aqueous interface and the homeotropic orientation is restored. This results in a bright-to-dark optical transition under a polarized optical microscope (POM). This optical transition serves as a visual indicator to mark the presence of NOX. The devised aptasensor demonstrates high specificity with a minimum detection limit of 5 nM (1.596 ppb). Moreover, the application of the developed aptasensor for the detection of NOX in freshwater and soil samples underscores its practical utility in environmental monitoring. This proposed LC-based method offers several advantages over conventional detection techniques for a rapid, feasible and convenient way to detect norfloxacin.

A cutting-edge electrochemical sensing platform for the simultaneous determination of the residues of antimicrobial drugs, rifampicin and norfloxacin, in water samples.

BACKGROUND: The widespread use and abuse of antibiotics has resulted in the pollution of water sources with antibiotic residues, posing a threat to human health, the environment, and the economy. Therefore, a highly sensitive and selective method is required for their detection in water samples. Herein, advanced ultrasensitive electrochemical sensor platform was developed by integrating gold-silver alloy nanocoral clusters (Au-Ag-ANCCs) with functionalized multi-walled carbon nanotube-carbon paste electrode (f-MWCNT-CPE) and choline chloride (ChCl) nanocomposites for simultaneously determining the residues of antimicrobial drugs, rifampicin (RAMP) and norfloxacin (NFX), in water samples. RESULTS: The developed sensor (Au-Ag-ANCCs/f-MWCNTs-CPE/ChCl) was extensively characterized using several analytical (UV-Vis, FT-IR, XRD, SEM, and EDX) and electrochemical (EIS, CV, and SWV) techniques. It exhibited outstanding performance in a wide linear range, from 14 pM to 115 µM for RAMP, and from 0.9 nM to 200 µM for NFX, with a limit of detection (LOD, 3σ/m, S/N = 3, n = 5) and a limit of quantification (LOQ, 10σ/m, S/N = 3, n = 5) values of 2.7 pM and 8.85 pM for RAMP, and 0.14 nM and 0.47 nM for NFX, respectively. The sensor also exhibited exceptional reproducibility, stability, and resistance to interference. SIGNIFICANCE: The developed sensor was effectively utilized to determine RAMP and NFX residues in hospital wastewater, river, and tap water samples, yielding recoveries within the range of 96.8-103 % and relative standard deviations below 5 %. Generally, the proposed sensor demonstrated remarkable performance in detecting the target analytes, making it an ideal tool and the first of its kind for addressing global antibiotic residue pollutants in water sources.

Monitoring Photo-Fenton and Photo-Electro-Fenton process of contaminants emerging concern by a gas diffusion electrode using Ca10-xFex-yWy(PO4)6(OH)2 nanoparticles as heterogeneous catalyst.

The catalytic performance of modified hydroxyapatite nanoparticles, Ca10-xFex-yWy(PO4)6(OH)2, was applied for the degradation of methylene blue (MB), fast green FCF (FG) and norfloxacin (NOR). XPS analysis pointed to the successful partial replacement of Ca by Fe. Under photo-electro-Fenton process, the catalyst Ca4FeII1·92W0·08FeIII4(PO4)6(OH)2 was combined with UVC radiation and electrogenerated H2O2 in a Printex L6 carbon-based gas diffusion electrode. The application of only 10 mA cm-2 resulted in 100% discoloration of MB and FG dyes in 50 min of treatment at pH 2.5, 7.0 and 9.0. The proposed treatment mechanism yielded maximum TOC removal of ∼80% and high mineralization current efficiency of ∼64%. Complete degradation of NOR was obtained in 40 min, and high mineralization of ∼86% was recorded after 240 min of treatment. Responses obtained from LC-ESI-MS/MS are in line with the theoretical Fukui indices and the ECOSAR data. The study enabled us to predict the main degradation route and the acute and chronic toxicity of the by-products formed during the contaminants degradation.

Bacterial nanocellulose-clay film as an eco-friendly sorbent for superior pollutants removal from aqueous solutions.

The persistent water treatment and separation challenge necessitates innovative and sustainable advances to tackle conventional and emerging contaminants in the aquatic environment effectively. Therefore, a unique three-dimensional (3D) network composite film (BNC-KC) comprised of bacterial nanocellulose (BNC) incorporated nano-kaolinite clay particles (KC) was successfully synthesized via an in-situ approach. The microscopic characterization of BNC-KC revealed an effective integration of KC within the 3D matrix of BNC. The investigated mechanical properties of BNC-KC demonstrated a better performance compared to BNC. Thereafter, the sorption performance of BNC-KC films towards basic blue 9 dye (Bb9) and norfloxacin (NFX) antibiotic from water was investigated. The maximum sorption capacities of BNC-KC for Bb9 and NFX were 127.64 and 101.68 mg/g, respectively. Mechanistic studies showed that electrostatic interactions, multi-layered sorption, and 3D structure are pivotal in the NFX/Bb9 sorption process. The intricate architecture of BNC-KC effectively traps molecules within the interlayer spaces, significantly increasing sorption efficiency. The distinctive structural configuration of BNC-KC films effectively addressed the challenges of post-water treatment separation while concurrently mitigating waste generation. The environmental evaluation, engineering, and economic feasibility of BNC-KC are also discussed. The cost estimation assessment of BNC-KC revealed the potential to remove NFX and Bb9 from water at an economically viable cost.

Impact of land use-induced soil heterogeneity on the adsorption of fluoroquinolone antibiotics, tested on organic matter pools.

The effects on the adsorption of fluoroquinolone antibiotics of long-term soil heterogeneity induced by land-use were investigated. Three different land use areas with their two organic matter (OM) pools were tested for the adsorption of three antibiotics widely detected in the environment (ciprofloxacin, norfloxacin, ofloxacin). The soils were separated into two size fractions, > 63 µm fraction and < 63 µm fractions for the fast and slow OM pools, respectively. Any effect of land use on adsorption was only observed in the slow pool in the increasing order: arable land, grassland, and forest. The composition of the soil organic matter (SOM) did influence adsorption in the slow pool, but not in the bulk soilsThis was, because: 1) the ratio of the slow pool was low, as in forest, 2) the ratio of the slow pool was high but its adsorption capacity was low due to its SOM composition, as in arable land and grassland. Soils containing a large slow SOM pool fraction with aliphatic dominance were found to be more likely to adsorb micropollutants. It is our contention that the release of contaminated water, sludge, manure or compost into the environment should only be undertaken after taking this into consideration.

Magnetic FNS/MILs nanofibers for highly efficient removal of norfloxacin via adsorption and Fenton-like reaction.

Iron-containing MOFs have attracted extensive interest as promising Fenton-like catalysts. In this work, magnetic Fe3O4 nanofiber (FNS)/MOFs composites with stable structure, included FNS/MIL-88B, FNS/MIL-88A and FNS/MIL-100, were prepared via the in-situ solvothermal method. The surface of the obtained fibers was covered by a dense and continuous MOFs layer, which could effectively solve the agglomeration problem of MOFs powder and improved the catalytic performance. The adsorption and catalytic properties of FNS/MOFs composites were evaluated by removal of norfloxacin. FNS/MIL-88B showed the best performance with a maximum adsorption capacity up to 214.09 mg/g, and could degrade 99% of NRF in 60 min. Meanwhile, FNS/MIL-88B had a saturation magnetization of 20 emu/g, and could be rapidly separated by an applied magnetic field. The self-supported nanofibers allowed the adequate contact between MOFs and pollutants, and promoted the catalytic activity and high stability. We believe that this work provided a new idea for the design and preparation of Fenton-like catalysts especially MOFs composites.