RESUMEN
Tigecycline is widely used for treating complicated bacterial infections for which there are no effective drugs. It inhibits bacterial protein translation by blocking the ribosomal A-site. However, even though it is also cytotoxic for human cells, the molecular mechanism of its inhibition remains unclear. Here, we present cryo-EM structures of tigecycline-bound human mitochondrial 55S, 39S, cytoplasmic 80S and yeast cytoplasmic 80S ribosomes. We find that at clinically relevant concentrations, tigecycline effectively targets human 55S mitoribosomes, potentially, by hindering A-site tRNA accommodation and by blocking the peptidyl transfer center. In contrast, tigecycline does not bind to human 80S ribosomes under physiological concentrations. However, at high tigecycline concentrations, in addition to blocking the A-site, both human and yeast 80S ribosomes bind tigecycline at another conserved binding site restricting the movement of the L1 stalk. In conclusion, the observed distinct binding properties of tigecycline may guide new pathways for drug design and therapy.
Asunto(s)
Microscopía por Crioelectrón , Ribosomas , Tigeciclina , Tigeciclina/farmacología , Tigeciclina/química , Humanos , Ribosomas/metabolismo , Ribosomas/efectos de los fármacos , Antibacterianos/farmacología , Antibacterianos/química , Sitios de Unión , Saccharomyces cerevisiae/efectos de los fármacos , Saccharomyces cerevisiae/metabolismo , Biosíntesis de Proteínas/efectos de los fármacos , Ribosomas Mitocondriales/metabolismo , Ribosomas Mitocondriales/química , Ribosomas Mitocondriales/efectos de los fármacos , Modelos Moleculares , ARN de Transferencia/metabolismo , ARN de Transferencia/químicaRESUMEN
The mitoribosome translates mitochondrial mRNAs and regulates energy conversion that is a signature of aerobic life forms. We present a 2.2 Å resolution structure of human mitoribosome together with validated mitoribosomal RNA (rRNA) modifications, including aminoacylated CP-tRNAVal. The structure shows how mitoribosomal proteins stabilise binding of mRNA and tRNA helping to align it in the decoding center, whereas the GDP-bound mS29 stabilizes intersubunit communication. Comparison between different states, with respect to tRNA position, allowed us to characterize a non-canonical L1 stalk, and molecular dynamics simulations revealed how it facilitates tRNA transitions in a way that does not require interactions with rRNA. We also report functionally important polyamines that are depleted when cells are subjected to an antibiotic treatment. The structural, biochemical, and computational data illuminate the principal functional components of the translation mechanism in mitochondria and provide a description of the structure and function of the human mitoribosome.
Asunto(s)
Ribosomas Mitocondriales , ARN de Transferencia , Humanos , ARN de Transferencia/metabolismo , ARN de Transferencia/química , ARN de Transferencia/genética , Ribosomas Mitocondriales/metabolismo , Ribosomas Mitocondriales/química , Ligandos , Simulación de Dinámica Molecular , ARN Mensajero/metabolismo , ARN Mensajero/genética , Mitocondrias/metabolismo , ARN Ribosómico/metabolismo , ARN Ribosómico/química , Proteínas Ribosómicas/metabolismo , Proteínas Ribosómicas/química , Guanosina Difosfato/metabolismo , Poliaminas/metabolismo , Poliaminas/química , Unión ProteicaRESUMEN
Ribosomes across species contain subsets of zinc finger proteins that play structural roles by binding to rRNA. While the majority of these zinc fingers belong to the C2-C2 type, the large subunit protein L36 in bacteria and mitochondria exhibits an atypical C2-CH motif. To comprehend the contribution of each coordinating residue in S. cerevisiae bL36m to mitoribosome assembly and function, we engineered and characterized strains carrying single and double mutations in the zinc coordinating residues. Our findings reveal that although all four residues markedly influence protein stability, C to A mutations in C66 and/or C69 have a more pronounced effect compared to those at C82 and H88. Importantly, protein stability directly correlates with the assembly and function of the mitoribosome and the growth rate of yeast in respiratory conditions. Mass spectrometry analysis of large subunit particles indicates that strains deleted for bL36m or expressing mutant variants have defective assembly of the L7/L12 stalk base, limiting their functional competence. Furthermore, we employed a synthetic bL36m protein collection, including both wild-type and mutant proteins, to elucidate their ability to bind zinc. Our data indicate that mutations in C82 and, particularly, H88 allow for some zinc binding albeit inefficient or unstable, explaining the residual accumulation and activity in mitochondria of bL36m variants carrying mutations in these residues. In conclusion, stable zinc binding by bL36m is essential for optimal mitoribosome assembly and function. MS data are available via ProteomeXchange with identifierPXD046465.
Asunto(s)
Ribosomas Mitocondriales , Saccharomyces cerevisiae , Ribosomas Mitocondriales/química , Ribosomas Mitocondriales/metabolismo , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo , Dedos de Zinc/genética , Subunidades Ribosómicas Grandes/genética , Zinc/metabolismoRESUMEN
Mitochondrial translation occurs on the mitochondrial ribosome, also known as the mitoribosome. The assembly of mitoribosomes is a highly coordinated process. During mitoribosome biogenesis, various assembly factors transiently associate with the nascent ribosome, facilitating the accurate and efficient construction of the mitoribosome. However, the specific factors involved in the assembly process, the precise mechanisms, and the cellular compartments involved in this vital process are not yet fully understood. In this study, we discovered a crucial role for GTP-binding protein 8 (GTPBP8) in the assembly of the mitoribosomal large subunit (mt-LSU) and mitochondrial translation. GTPBP8 is identified as a novel GTPase located in the matrix and peripherally bound to the inner mitochondrial membrane. Importantly, GTPBP8 is specifically associated with the mt-LSU during its assembly. Depletion of GTPBP8 leads to an abnormal accumulation of mt-LSU, indicating that GTPBP8 is critical for proper mt-LSU assembly. Furthermore, the absence of GTPBP8 results in reduced levels of fully assembled 55S monosomes. This impaired assembly leads to compromised mitochondrial translation and, consequently, impaired mitochondrial function. The identification of GTPBP8 as an important player in these processes provides new insights into the molecular mechanisms underlying mitochondrial protein synthesis and its regulation.
Asunto(s)
Mitocondrias , Membranas Mitocondriales , Mitocondrias/metabolismo , Membranas Mitocondriales/metabolismo , Ribosomas Mitocondriales/química , Ribosomas Mitocondriales/metabolismo , Biosíntesis de Proteínas , Proteínas de Unión al GTP/genética , Proteínas de Unión al GTP/metabolismo , Proteínas Mitocondriales/metabolismo , Proteínas Ribosómicas/genética , Proteínas Ribosómicas/metabolismoRESUMEN
Mitochondrial ribosomes (mitoribosomes) synthesize proteins encoded within the mitochondrial genome that are assembled into oxidative phosphorylation complexes. Thus, mitoribosome biogenesis is essential for ATP production and cellular metabolism1. Here we used cryo-electron microscopy to determine nine structures of native yeast and human mitoribosomal small subunit assembly intermediates, illuminating the mechanistic basis for how GTPases are used to control early steps of decoding centre formation, how initial rRNA folding and processing events are mediated, and how mitoribosomal proteins have active roles during assembly. Furthermore, this series of intermediates from two species with divergent mitoribosomal architecture uncovers both conserved principles and species-specific adaptations that govern the maturation of mitoribosomal small subunits in eukaryotes. By revealing the dynamic interplay between assembly factors, mitoribosomal proteins and rRNA that are required to generate functional subunits, our structural analysis provides a vignette for how molecular complexity and diversity can evolve in large ribonucleoprotein assemblies.
Asunto(s)
Microscopía por Crioelectrón , Ribosomas Mitocondriales , Ribonucleoproteínas , Subunidades Ribosómicas Pequeñas , Saccharomyces cerevisiae , Humanos , Proteínas Mitocondriales/química , Proteínas Mitocondriales/metabolismo , Proteínas Mitocondriales/ultraestructura , Ribosomas Mitocondriales/química , Ribosomas Mitocondriales/metabolismo , Ribosomas Mitocondriales/ultraestructura , Proteínas Ribosómicas/química , Proteínas Ribosómicas/metabolismo , Proteínas Ribosómicas/ultraestructura , Saccharomyces cerevisiae/citología , Saccharomyces cerevisiae/metabolismo , ARN Ribosómico , GTP Fosfohidrolasas , Ribonucleoproteínas/química , Ribonucleoproteínas/metabolismo , Ribonucleoproteínas/ultraestructura , Proteínas Fúngicas/química , Proteínas Fúngicas/metabolismo , Proteínas Fúngicas/ultraestructura , Subunidades Ribosómicas Pequeñas/química , Subunidades Ribosómicas Pequeñas/metabolismo , Subunidades Ribosómicas Pequeñas/ultraestructuraRESUMEN
Translation in mitochondria is mediated by mitochondrial ribosomes, or mitoribosomes, complex ribonucleoprotein machines with dual genetic origin. Mitoribosomes in trypanosomatid parasites diverged markedly from their bacterial ancestors and other eukaryotic lineages in terms of protein composition, rRNA content, and overall architecture, yet their core functional elements remained conserved. Recent cryo-electron microscopy studies provided atomic models of trypanosomatid large and small mitoribosomal subunits and their precursors, making these parasites the organisms with the best-understood biogenesis of mitoribosomes. The structures revealed molecular mechanisms and players involved in the assembly of mitoribosomes not only in the parasites, but also in eukaryotes in general.
Asunto(s)
Ribosomas Mitocondriales , ARN Ribosómico , Microscopía por Crioelectrón , Ribosomas Mitocondriales/química , Ribosomas Mitocondriales/metabolismo , ARN Ribosómico/análisis , ARN Ribosómico/química , ARN Ribosómico/metabolismo , Mitocondrias/metabolismoRESUMEN
Mitoribosomes are essential for the synthesis and maintenance of bioenergetic proteins. Here we use cryo-electron microscopy to determine a series of the small mitoribosomal subunit (SSU) intermediates in complex with auxiliary factors, revealing a sequential assembly mechanism. The methyltransferase TFB1M binds to partially unfolded rRNA h45 that is promoted by RBFA, while the mRNA channel is blocked. This enables binding of METTL15 that promotes further rRNA maturation and a large conformational change of RBFA. The new conformation allows initiation factor mtIF3 to already occupy the subunit interface during the assembly. Finally, the mitochondria-specific ribosomal protein mS37 (ref. 1) outcompetes RBFA to complete the assembly with the SSU-mS37-mtIF3 complex2 that proceeds towards mtIF2 binding and translation initiation. Our results explain how the action of step-specific factors modulate the dynamic assembly of the SSU, and adaptation of a unique protein, mS37, links the assembly to initiation to establish the catalytic human mitoribosome.
Asunto(s)
Ribosomas Mitocondriales , Subunidades Ribosómicas Pequeñas , Humanos , Microscopía por Crioelectrón , Proteínas de Unión al ADN/química , Proteínas de Unión al ADN/metabolismo , Factores Eucarióticos de Iniciación/química , Factores Eucarióticos de Iniciación/metabolismo , Mitocondrias/química , Mitocondrias/metabolismo , Proteínas Mitocondriales/química , Proteínas Mitocondriales/metabolismo , Ribosomas Mitocondriales/química , Ribosomas Mitocondriales/metabolismo , Ribosomas Mitocondriales/ultraestructura , Proteínas Ribosómicas/química , Proteínas Ribosómicas/metabolismo , Subunidades Ribosómicas Pequeñas/química , Subunidades Ribosómicas Pequeñas/metabolismo , Subunidades Ribosómicas Pequeñas/ultraestructura , ARN Ribosómico/química , ARN Ribosómico/metabolismo , Proteínas de Unión al ARN/química , Proteínas de Unión al ARN/metabolismo , Factores de Transcripción/química , Factores de Transcripción/metabolismoRESUMEN
Mitochondrial ribosomes (mitoribosomes) play a central role in synthesizing mitochondrial inner membrane proteins responsible for oxidative phosphorylation. Although mitoribosomes from different organisms exhibit considerable structural variations, recent insights into mitoribosome assembly suggest that mitoribosome maturation follows common principles and involves a number of conserved assembly factors. To investigate the steps involved in the assembly of the mitoribosomal small subunit (mt-SSU) we determined the cryoelectron microscopy structures of middle and late assembly intermediates of the Trypanosoma brucei mitochondrial small subunit (mt-SSU) at 3.6- and 3.7-Å resolution, respectively. We identified five additional assembly factors that together with the mitochondrial initiation factor 2 (mt-IF-2) specifically interact with functionally important regions of the rRNA, including the decoding center, thereby preventing premature mRNA or large subunit binding. Structural comparison of assembly intermediates with mature mt-SSU combined with RNAi experiments suggests a noncanonical role of mt-IF-2 and a stepwise assembly process, where modular exchange of ribosomal proteins and assembly factors together with mt-IF-2 ensure proper 9S rRNA folding and protein maturation during the final steps of assembly.
Asunto(s)
Proteínas Mitocondriales/química , Ribosomas Mitocondriales/química , Fosforilación Oxidativa , ARN Ribosómico/química , Proteínas Ribosómicas/química , Subunidades Ribosómicas/química , Línea Celular , Microscopía por Crioelectrón , Mitocondrias/metabolismo , Proteínas Mitocondriales/genética , Proteínas Mitocondriales/metabolismo , Ribosomas Mitocondriales/metabolismo , Modelos Moleculares , ARN Ribosómico/genética , ARN Ribosómico/metabolismo , Proteínas Ribosómicas/genética , Proteínas Ribosómicas/metabolismo , Subunidades Ribosómicas/genética , Subunidades Ribosómicas/metabolismo , Trypanosoma brucei brucei/genética , Trypanosoma brucei brucei/metabolismoRESUMEN
Mitochondria are involved in a variety of critical cellular functions, and their impairment drives cell injury. The mitochondrial ribosome (mitoribosome) is responsible for the protein synthesis of mitochondrial DNA-encoded genes. These proteins are involved in oxidative phosphorylation, respiration, and ATP production required in the cell. Mitoribosome components originate from both mitochondrial and nuclear genomes. Their dysfunction can be caused by impaired mitochondrial protein synthesis or mitoribosome misassembly, leading to a decline in mitochondrial translation. This decrease can trigger mitochondrial ribosomal stress and contribute to pulmonary cell injury, death, and diseases. This review focuses on the contribution of the impaired mitoribosome structural components and function to respiratory disease pathophysiology. We present recent findings in the fields of lung cancer, chronic obstructive pulmonary disease, interstitial lung disease, and asthma. We also include reports on the mitoribosome dysfunction in pulmonary hypertension, high-altitude pulmonary edema, and bacterial and viral infections. Studies of the mitoribosome alterations in respiratory diseases can lead to novel therapeutic targets.
Asunto(s)
Enfermedades Pulmonares , Ribosomas Mitocondriales , Humanos , Enfermedades Pulmonares/metabolismo , Mitocondrias/metabolismo , Proteínas Mitocondriales/metabolismo , Ribosomas Mitocondriales/química , Ribosomas Mitocondriales/metabolismo , Biosíntesis de ProteínasRESUMEN
Final maturation steps during ribosome biogenesis require the assistance of assembly and quality control factors to ensure the folding of rRNA and proteins into a functional translation machinery. Here we integrate several recent structural snapshots of native large ribosomal subunit intermediates into the complex pathway of mitochondrial ribosome assembly.
Asunto(s)
Ribosomas Mitocondriales , Ribosomas , Dominio Catalítico , Humanos , Ribosomas Mitocondriales/química , Ribosomas Mitocondriales/metabolismo , Biogénesis de Organelos , ARN Ribosómico/metabolismo , Proteínas Ribosómicas/análisis , Proteínas Ribosómicas/genética , Proteínas Ribosómicas/metabolismo , Ribosomas/metabolismoRESUMEN
In Saccharomyces cerevisiae, mitoribosomes are composed of a 54S large subunit (mtLSU) and a 37S small subunit (mtSSU). The two subunits altogether contain 73 mitoribosome proteins (MRPs) and two ribosomal RNAs (rRNAs). Although mitoribosomes preserve some similarities with their bacterial counterparts, they have significantly diverged by acquiring new proteins, protein extensions, and new RNA segments, adapting the mitoribosome to the synthesis of highly hydrophobic membrane proteins. In this study, we investigated the functional relevance of mitochondria-specific protein extensions at the C-terminus (C) or N-terminus (N) present in 19 proteins of the mtLSU. The studied mitochondria-specific extensions consist of long tails and loops extending from globular domains that mainly interact with mitochondria-specific proteins and 21S rRNA moieties extensions. The expression of variants devoid of extensions in uL4 (C), uL5 (N), uL13 (N), uL13 (C), uL16 (C), bL17 (N), bL17 (C), bL21 (24), uL22 (N), uL23 (N), uL23 (C), uL24 (C), bL27 (C), bL28 (N), bL28 (C), uL29 (N), uL29 (C), uL30 (C), bL31 (C), and bL32 (C) did not rescue the mitochondrial protein synthesis capacities and respiratory growth of the respective null mutants. On the contrary, the truncated form of the mitoribosome exit tunnel protein uL24 (N) yields a partially functional mitoribosome. Also, the removal of mitochondria-specific sequences from uL1 (N), uL3 (N), uL16 (N), bL9 (N), bL19 (C), uL29 (C), and bL31 (N) did not affect the mitoribosome function and respiratory growth. The collection of mutants described here provides new means to study and evaluate defective assembly modules in the mitoribosome biogenesis process.
Asunto(s)
Mitocondrias , Ribosomas Mitocondriales , Mitocondrias/genética , Mitocondrias/metabolismo , Proteínas Mitocondriales/genética , Ribosomas Mitocondriales/química , Ribosomas Mitocondriales/metabolismo , Biosíntesis de Proteínas , Proteínas Ribosómicas/genética , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismoRESUMEN
Mitochondria are the powerhouse of eukaryotic cells. They possess their own gene expression machineries where highly divergent and specialized ribosomes, named hereafter mitoribosomes, translate the few essential messenger RNAs still encoded by mitochondrial genomes. Here, we present a biochemical and structural characterization of the mitoribosome in the model green alga Chlamydomonas reinhardtii, as well as a functional study of some of its specific components. Single particle cryo-electron microscopy resolves how the Chlamydomonas mitoribosome is assembled from 13 rRNA fragments encoded by separate non-contiguous gene pieces. Additional proteins, mainly OPR, PPR and mTERF helical repeat proteins, are found in Chlamydomonas mitoribosome, revealing the structure of an OPR protein in complex with its RNA binding partner. Targeted amiRNA silencing indicates that these ribosomal proteins are required for mitoribosome integrity. Finally, we use cryo-electron tomography to show that Chlamydomonas mitoribosomes are attached to the inner mitochondrial membrane via two contact points mediated by Chlamydomonas-specific proteins. Our study expands our understanding of mitoribosome diversity and the various strategies these specialized molecular machines adopt for membrane tethering.
Asunto(s)
Chlamydomonas reinhardtii/metabolismo , Mitocondrias/metabolismo , ARN/metabolismo , Ribosomas/metabolismo , Chlamydomonas reinhardtii/química , Chlamydomonas reinhardtii/genética , Chlamydomonas reinhardtii/ultraestructura , Microscopía por Crioelectrón , Mitocondrias/química , Mitocondrias/genética , Mitocondrias/ultraestructura , Proteínas Mitocondriales , Ribosomas Mitocondriales/química , Ribosomas Mitocondriales/metabolismo , Ribosomas Mitocondriales/ultraestructura , ARN/química , ARN/genética , ARN/ultraestructura , Proteínas Ribosómicas/química , Proteínas Ribosómicas/genética , Proteínas Ribosómicas/metabolismo , Proteínas Ribosómicas/ultraestructura , Ribosomas/química , Ribosomas/genética , Ribosomas/ultraestructuraRESUMEN
Mitochondrial ribosomes (mitoribosomes) synthesize a critical set of proteins essential for oxidative phosphorylation. Therefore, mitoribosomal function is vital to the cellular energy supply. Mitoribosome biogenesis follows distinct molecular pathways that remain poorly understood. Here, we determine the cryo-EM structures of mitoribosomes isolated from human cell lines with either depleted or overexpressed mitoribosome assembly factor GTPBP5, allowing us to capture consecutive steps during mitoribosomal large subunit (mt-LSU) biogenesis. Our structures provide essential insights into the last steps of 16S rRNA folding, methylation and peptidyl transferase centre (PTC) completion, which require the coordinated action of nine assembly factors. We show that mammalian-specific MTERF4 contributes to the folding of 16S rRNA, allowing 16 S rRNA methylation by MRM2, while GTPBP5 and NSUN4 promote fine-tuning rRNA rearrangements leading to PTC formation. Moreover, our data reveal an unexpected involvement of the elongation factor mtEF-Tu in mt-LSU assembly, where mtEF-Tu interacts with GTPBP5, similar to its interaction with tRNA during translational elongation.
Asunto(s)
Ribosomas Mitocondriales/química , Subunidades Ribosómicas Grandes/química , Línea Celular , Microscopía por Crioelectrón , Humanos , Metiltransferasas/química , Metiltransferasas/metabolismo , Ribosomas Mitocondriales/metabolismo , Modelos Moleculares , Proteínas de Unión al GTP Monoméricas/química , Proteínas de Unión al GTP Monoméricas/metabolismo , Complejos Multiproteicos , Factor Tu de Elongación Peptídica/química , Factor Tu de Elongación Peptídica/metabolismo , Peptidil Transferasas/química , Peptidil Transferasas/metabolismo , Unión Proteica , Pliegue del ARN , ARN Ribosómico 16S/química , ARN Ribosómico 16S/metabolismo , Subunidades Ribosómicas Grandes/metabolismo , Factores de Transcripción/química , Factores de Transcripción/metabolismoRESUMEN
Ribosome biogenesis requires auxiliary factors to promote folding and assembly of ribosomal proteins and RNA. Particularly, maturation of the peptidyl transferase center (PTC) is mediated by conserved GTPases, but the molecular basis is poorly understood. Here, we define the mechanism of GTPase-driven maturation of the human mitochondrial large ribosomal subunit (mtLSU) using endogenous complex purification, in vitro reconstitution and cryo-EM. Structures of transient native mtLSU assembly intermediates that accumulate in GTPBP6-deficient cells reveal how the biogenesis factors GTPBP5, MTERF4 and NSUN4 facilitate PTC folding. Addition of recombinant GTPBP6 reconstitutes late mtLSU biogenesis in vitro and shows that GTPBP6 triggers a molecular switch and progression to a near-mature PTC state. Additionally, cryo-EM analysis of GTPBP6-treated mature mitochondrial ribosomes reveals the structural basis for the dual-role of GTPBP6 in ribosome biogenesis and recycling. Together, these results provide a framework for understanding step-wise PTC folding as a critical conserved quality control checkpoint.
Asunto(s)
Proteínas de Unión al GTP/química , Ribosomas Mitocondriales/química , Proteínas de Unión al GTP Monoméricas/química , Microscopía por Crioelectrón , Proteínas de Unión al GTP/genética , Proteínas de Unión al GTP/metabolismo , Humanos , Metiltransferasas/química , Metiltransferasas/metabolismo , Ribosomas Mitocondriales/metabolismo , Modelos Moleculares , Proteínas de Unión al GTP Monoméricas/metabolismo , Complejos Multiproteicos , Biogénesis de Organelos , Peptidil Transferasas/química , Peptidil Transferasas/metabolismo , Pliegue de Proteína , ARN Ribosómico/química , ARN Ribosómico/metabolismo , Subunidades Ribosómicas Grandes/química , Subunidades Ribosómicas Grandes/metabolismo , Factores de Transcripción/química , Factores de Transcripción/metabolismoRESUMEN
Mitochondrial ribosomes are specialized for the synthesis of membrane proteins responsible for oxidative phosphorylation. Mammalian mitoribosomes have diverged considerably from the ancestral bacterial ribosomes and feature dramatically reduced ribosomal RNAs. The structural basis of the mammalian mitochondrial ribosome assembly is currently not well understood. Here we present eight distinct assembly intermediates of the human large mitoribosomal subunit involving seven assembly factors. We discover that the NSUN4-MTERF4 dimer plays a critical role in the process by stabilizing the 16S rRNA in a conformation that exposes the functionally important regions of rRNA for modification by the MRM2 methyltransferase and quality control interactions with the conserved mitochondrial GTPase MTG2 that contacts the sarcin-ricin loop and the immature active site. The successive action of these factors leads to the formation of the peptidyl transferase active site of the mitoribosome and the folding of the surrounding rRNA regions responsible for interactions with tRNAs and the small ribosomal subunit.
Asunto(s)
Ribosomas Mitocondriales/química , Peptidil Transferasas/química , Dominio Catalítico , Microscopía por Crioelectrón , Humanos , Metiltransferasas/química , Metiltransferasas/metabolismo , Ribosomas Mitocondriales/metabolismo , Modelos Moleculares , Proteínas de Unión al GTP Monoméricas/química , Proteínas de Unión al GTP Monoméricas/metabolismo , Conformación de Ácido Nucleico , Peptidil Transferasas/metabolismo , Multimerización de Proteína , ARN Ribosómico/química , ARN Ribosómico/metabolismo , Subunidades Ribosómicas Grandes/química , Subunidades Ribosómicas Grandes/metabolismo , Factores de Transcripción/metabolismoRESUMEN
Ribosomes are recycled for a new round of translation initiation by dissociation of ribosomal subunits, messenger RNA and transfer RNA from their translational post-termination complex. Here we present cryo-EM structures of the human 55S mitochondrial ribosome (mitoribosome) and the mitoribosomal large 39S subunit in complex with mitoribosome recycling factor (RRFmt) and a recycling-specific homolog of elongation factor G (EF-G2mt). These structures clarify an unusual role of a mitochondria-specific segment of RRFmt, identify the structural distinctions that confer functional specificity to EF-G2mt, and show that the deacylated tRNA remains with the dissociated 39S subunit, suggesting a distinct sequence of events in mitoribosome recycling. Furthermore, biochemical and structural analyses reveal that the molecular mechanism of antibiotic fusidic acid resistance for EF-G2mt is markedly different from that of mitochondrial elongation factor EF-G1mt, suggesting that the two human EF-Gmts have evolved diversely to negate the effect of a bacterial antibiotic.
Asunto(s)
Farmacorresistencia Microbiana/genética , Ribosomas Mitocondriales/química , Ribosomas Mitocondriales/metabolismo , Ribosomas/química , Ribosomas/metabolismo , Microscopía por Crioelectrón , Humanos , Mitocondrias , Ribosomas Mitocondriales/efectos de los fármacos , Modelos Moleculares , Factor G de Elongación Peptídica/química , Factor G de Elongación Peptídica/genética , Factor G de Elongación Peptídica/metabolismo , Conformación Proteica , Conformación Proteica en Hélice alfa , Dominios Proteicos , ARN de Transferencia/química , ARN de Transferencia/metabolismo , Proteínas Ribosómicas/química , Proteínas Ribosómicas/genética , Proteínas Ribosómicas/metabolismo , Ribosomas/genéticaRESUMEN
Mitochondrial ribosomes (mitoribosomes) are specialized machineries that carry out the synthesis of a limited number of proteins encoded in the mitochondrial genome, including components of the oxidative phosphorylation pathway. They have incorporated several structural features distinguishing them from bacterial and eukaryotic cytosolic counterparts. Our current understanding of the assembly and functioning of mitoribosomes is limited, and recent developments in cryo-EM provide promising directions for detailed investigation. Here we describe methods to purify mitoribosomes from human embryonic kidney cells for cryo-EM studies.
Asunto(s)
Microscopía por Crioelectrón/métodos , Ribosomas Mitocondriales/química , Ribosomas Mitocondriales/ultraestructura , Complejo I de Transporte de Electrón/metabolismo , Procesamiento Automatizado de Datos , Genoma Mitocondrial , Células HEK293 , Humanos , Proteínas Mitocondriales/metabolismo , Ribosomas Mitocondriales/metabolismo , Fosforilación Oxidativa , Biosíntesis de Proteínas , Proteínas Ribosómicas/metabolismoRESUMEN
Mitochondria contain ribosomes (mitoribosomes) specialized in the synthesis of a handful of proteins essential for oxidative phosphorylation. Therefore, mitoribosome integrity and function are essential for the life of eukaryotic cells and lesions that affect them result in devastating human disorders. To broadly analyze the integrity and assembly state of mitoribosomes it is useful to start by determining the sedimentation profile of these structures by sucrose gradient centrifugation of mitochondrial extracts. During centrifugation, mitoribosome subunits, monosomes and polysomes, and potentially accumulated assembly intermediates will sediment through the gradient at different rates. Sedimentation will depend on the centrifugal force applied and the density and viscosity of the gradient. Importantly, it will also depend on the size, shape, and density of the mitoribosome particles present in the samples under study. Variations of this technique, often coupled with additional downstream approaches, have been used to analyze the process of mitoribosome biogenesis, the composition of assembly intermediates, or to monitor the interaction of extraribosomal proteins with individual mitoribosome subunits or monosomes.
Asunto(s)
Centrifugación por Gradiente de Densidad/métodos , ARN Helicasas DEAD-box/química , Proteínas Mitocondriales/química , Ribosomas Mitocondriales/química , Proteínas Ribosómicas/química , Células HEK293 , Humanos , Proteínas Mitocondriales/metabolismo , Ribosomas Mitocondriales/metabolismo , Fosforilación Oxidativa , Biosíntesis de Proteínas , Proteínas Ribosómicas/metabolismoRESUMEN
The human mitochondrial ribosome (mitoribosome) and associated proteins regulate the synthesis of 13 essential subunits of the oxidative phosphorylation complexes. We report the discovery of a mitoribosome-associated quality control pathway that responds to interruptions during elongation, and we present structures at 3.1- to 3.3-angstrom resolution of mitoribosomal large subunits trapped during ribosome rescue. Release factor homolog C12orf65 (mtRF-R) and RNA binding protein C6orf203 (MTRES1) eject the nascent chain and peptidyl transfer RNA (tRNA), respectively, from stalled ribosomes. Recruitment of mitoribosome biogenesis factors to these quality control intermediates suggests additional roles for these factors during mitoribosome rescue. We also report related cryo-electron microscopy structures (3.7 to 4.4 angstrom resolution) of elongating mitoribosomes bound to tRNAs, nascent polypeptides, the guanosine triphosphatase elongation factors mtEF-Tu and mtEF-G1, and the Oxa1L translocase.
Asunto(s)
Ribosomas Mitocondriales/química , Elongación de la Transcripción Genética , Microscopía por Crioelectrón , Complejo IV de Transporte de Electrones/química , Escherichia coli , Exorribonucleasas/genética , Células HEK293 , Humanos , Proteínas Mitocondriales/química , Proteínas Nucleares/química , Factores de Terminación de Péptidos/química , Dominios Proteicos , ARN de Transferencia/química , Proteínas de Unión al ARN/química , Proteínas Ribosómicas/química , Factores de Elongación Transcripcional/químicaRESUMEN
Mitochondria are eukaryotic organelles of bacterial origin where respiration takes place to produce cellular chemical energy. These reactions are catalyzed by the respiratory chain complexes located in the inner mitochondrial membrane. Notably, key components of the respiratory chain complexes are encoded on the mitochondrial chromosome and their expression relies on a dedicated mitochondrial translation machinery. Defects in the mitochondrial gene expression machinery lead to a variety of diseases in humans mostly affecting tissues with high energy demand such as the nervous system, the heart, or the muscles. The mitochondrial translation system has substantially diverged from its bacterial ancestor, including alterations in the mitoribosomal architecture, multiple changes to the set of translation factors and striking reductions in otherwise conserved tRNA elements. Although a number of structures of mitochondrial ribosomes from different species have been determined, our mechanistic understanding of the mitochondrial translation cycle remains largely unexplored. Here, we present two cryo-EM reconstructions of human mitochondrial elongation factor G1 bound to the mammalian mitochondrial ribosome at two different steps of the tRNA translocation reaction during translation elongation. Our structures explain the mechanism of tRNA and mRNA translocation on the mitoribosome, the regulation of mtEFG1 activity by the ribosomal GTPase-associated center, and the basis of decreased susceptibility of mtEFG1 to the commonly used antibiotic fusidic acid.