RESUMEN
BACKGROUND: Sambucus williamsii Hance (SWH) has effectively been adopted to treat joint and bone disorders. Diabetes-induced osteopenia (DOP) is caused primarily by impaired bone formation as a result of hyperglycemia. We had previously demonstrated that SWH extract accelerated fracture healing and promoted osteoblastic MC3T3-E1 cell proliferation and osteogenic differentiation. This study assessed the impacts of SWH extract on diabetes-induced bone loss and explored the mechanisms underlying its osteoprotective effects. METHODS: This work employed MC3T3-E1 cell line for evaluating how SWH extract affected osteogenesis, oxidative stress (OS), and the underlying mechanism in vitro. Streptozotocin-induced osteopenia mouse model was applied with the purpose of assessing SWH extract's osteoprotection on bone homeostasis in vivo. RESULTS: The increased OS of MC3T3-E1 cells exposed to high glucose (HG) was largely because of the upregulation of pro-oxidant genes and the downregulation of antioxidant genes, whereas SWH extract reduced the OS by modulating NADPH oxidase-4 and thioredoxin-related genes by activating cyclic guanosine monophosphate (cGMP) production and increasing the level of cGMP-mediated protein kinase G type-2 (PKG2). The oral administration of SWH extract maintained bone homeostasis in type 1 diabetes mellitus (T1DM) mice by enhancing osteogenesis while decreasing OS. In bones from hyperglycemia-induced osteopenia mice and HG-treated MC3T3-E1 cells, the SWH extract achieved the osteoprotective effects through activating the cGMP/PKG2 signaling pathway, upregulating the level of antioxidant genes, as well as downregulating the level of pro-oxidant genes. CONCLUSION: SWH extract exerts osteoprotective effects on hyperglycemia-induced osteopenia by reversing OS via cGMP/PKG signal transduction and is a potential therapy for DOP.
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Enfermedades Óseas Metabólicas , Hiperglucemia , Sambucus , Animales , Ratones , Antioxidantes/farmacología , Antioxidantes/metabolismo , Enfermedades Óseas Metabólicas/tratamiento farmacológico , Enfermedades Óseas Metabólicas/etiología , Enfermedades Óseas Metabólicas/metabolismo , Homeostasis , Hiperglucemia/tratamiento farmacológico , Hiperglucemia/metabolismo , Osteoblastos , Osteogénesis , Estrés Oxidativo , Especies Reactivas de Oxígeno/metabolismo , Sambucus/metabolismo , Transducción de Señal , GMP Cíclico/metabolismo , Proteínas Quinasas Dependientes de GMP Cíclico/metabolismoRESUMEN
BACKGROUND: The chemical composition, phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO) and peroxidase (POD) activity of the three main Portuguese elderberry cultivars were determined for the first time through five stages of maturation, in different harvesting years, to gain a deeper understanding of the effect of climatic conditions and enzymatic activity involved in the synthesis and degradation of phenolic compounds on the final quality of elderberries. RESULTS: Simple sugar and anthocyanin content increased with maturation but total acidity and flavonoids content decreased, and cinnamic acids did not show a clear trend. Climatic conditions seem to have a decisive influence on the elderberry maturation, namely the total number of hot (>30 °C) days. The PAL, PPO, and POD activity can explain the differences observed in elderberry phenolic content. CONCLUSION: These results highlighted the influence of climatic conditions in each harvesting season on elderberry development and quality. © 2022 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Asunto(s)
Sambucus , Sambucus/química , Sambucus/metabolismo , Azúcares/análisis , Fenoles/análisis , Antioxidantes/análisis , Frutas/químicaRESUMEN
Over the past years, several studies have also reported the adverse effects of hyperthermia on normal testicular tissues in several species including mice, rats, and humans. These deleterious impacts include temporarily drop in relative weight of testis along with a temporary partial or complete infertility. Sambucus nigra, also known as elderberry or sweet elder, is a source of bioactive compounds that has drawn growing attention for its potential beneficial effects in preventing and treating several diseases. This experimental research divided 30 mice into the following three groups: (1) control, (2) hyperthermia, and (3) hyperthermia receiving elderberry diet for 35 days. Scrotal hyperthermia was induced by water bath with 43 °C for 30 min. Then, the mice were euthanized, and their sperm samples were collected for sperm parameters analysis. Then, we took the testis samples for histopathological experimentations, immunohistochemistry against TNF-α and caspase-3 and serum testosterone, FSH and LH levels. Our outputs indicated that elderberry diet could largely improve the sperms parameters and stereological parameters, like spermatogonia, primary spermatocyte, round spermatid, and Leydig cells together with an increasing level of the serum testosterone compared to the scrotal hyperthermia induced mice. In addition, it was found that the expression of TNF-α and caspase-3 significantly decreased in the treatment groups by elderberry diet compared to the scrotal hyperthermia-induced mice. In conclusion, it could be concluded that elderberry diet may be regarded as an alternative treatment for improving the spermatogenesis process in the scrotal hyperthermia induced mice.
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Sambucus , Animales , Masculino , Ratones , Caspasa 3/metabolismo , Dieta , Sambucus/metabolismo , Semillas/metabolismo , Espermatogénesis , Espermatozoides/metabolismo , Testículo/metabolismo , Testosterona , Factor de Necrosis Tumoral alfa/metabolismoRESUMEN
In this study, encoding sequence of a new type 2 RIP (pebulin) was isolated and cloned from dwarf elder (Sambucus ebulus L.) native to the northern regions of Iran. The nucleotide sequence of pebulin was ligated to the pET-28a(+) expression plasmid and cloned into the E. coli strain BL21 (DE3) in order to express heterologously of recombinant protein. The recombinant pebulin protein was mainly produced in the form of insoluble inclusion bodies probably because to absence of N-glycosylation process in E. coli. Therefore, in order to increase the expression of recombinant protein in soluble form, co-expression of the target protein with the pG-Tf2 chaperone plasmid and incubation of bacterial culture under low temperature were used to enhance solubility and accumulation of recombinant protein. After purification of the recombinant protein using affinity chromatography method, the bioactivity of pebulin was analyzed by hemagglutination, anticancer, and antifungal assays. The results of the hemagglutination assay showed that purified pebulin agglutinated erythrocytes in all human blood groups. In addition, pebulin considerably inhibited the proliferation of cancer cell lines MCF-7 and HT-29 in a time- and dose-dependent manner and indicated remarkably growth-inhibiting effect against the plant pathogenic fungi such as Alternaria solani and Fusarium oxysporum.
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Antifúngicos/farmacología , Antineoplásicos Fitogénicos/farmacología , Proteínas Recombinantes/farmacología , Proteínas Inactivadoras de Ribosomas Tipo 2/genética , Sambucus/metabolismo , Alternaria/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Cromatografía de Afinidad , Clonación Molecular , Escherichia coli/genética , Escherichia coli/crecimiento & desarrollo , Fusarium/efectos de los fármacos , Células HT29 , Humanos , Células MCF-7 , Pruebas de Sensibilidad Microbiana , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plásmidos/genética , Proteínas Inactivadoras de Ribosomas Tipo 2/metabolismo , Sambucus/genéticaRESUMEN
Glycoforms are otherwise identical proteins with different glycosylation. A lectin, Sambucus sieboldiana agglutinin (SSA), specifically binds glycoforms having α2,6-sialyl residues. The binding is found to inhibit antigen-antibody reaction; e.g., SSA inhibits anti-transferrin antibody binding to α2,6-sialylated transferrin (Tf) (SSA inhibition). SSA inhibition is not observed with other Tf glycoforms, indicating that the inhibition is glycoform-specific. Here we describe the application of SSA inhibition to ELISA as a specific assay for quantifying α2,6-sialylated Tf.
Asunto(s)
Lectinas de Plantas/farmacología , Proteínas Inactivadoras de Ribosomas/farmacología , Sambucus/metabolismo , Transferrina/análisis , Transferrina/química , Anticuerpos/metabolismo , Reacciones Antígeno-Anticuerpo/efectos de los fármacos , Análisis Químico de la Sangre , Ensayo de Inmunoadsorción Enzimática , Glicosilación , Humanos , Ácido N-Acetilneuramínico/metabolismo , Transferrina/inmunologíaRESUMEN
Elderberries are good sources of anthocyanins, which are poorly absorbed in the upper gastrointestinal tract but extensively transformed into phenolic metabolites at the colonic level. Because different gut microbiota strains have different metabolism, the catabolism of anthocyanins may lead to interindividual differences in metabolite production. In this work, an anthocyanin-rich elderberry extract was incubated with three single gut microbial strains (Enterobacter cancerogenous, Bifidobacterium dentium, and Dorea longicatena) up to 4 days, to assess differences in their phenolic metabolism. All of the strains degraded the elderberry anthocyanins, but the metabolic pathways followed were different. Although some metabolites were common for all of the strains, a wide disparity was observed in the kind and amount of several phenolic metabolites produced by each species. These in vitro preliminary results may be of help in the interpretation of the bioavailability of anthocyanins and give a clue to understand interindividual variability in metabolite production.
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Antocianinas/metabolismo , Bifidobacterium/metabolismo , Clostridiales/metabolismo , Enterobacter/metabolismo , Microbioma Gastrointestinal , Extractos Vegetales/metabolismo , Sambucus/metabolismo , Colon/metabolismo , Colon/microbiología , Frutas/metabolismo , Humanos , Redes y Vías MetabólicasRESUMEN
In this study we explored, for the first time, the lactic acid fermentation of elderberry juice (EJ). A total of 15 strains isolated from dairy and plant matrices, belonging to L. plantarum, L. rhamnosus and L. casei, were used for fermentations. The volatile profile of started and unstarted EJ was characterized by HS-SPME/GC-MS technique after 48h of fermentation and 12days of storage at 4°C. All L. plantarum and L. rhamnosus strains exhibited a good capacity of growth while not all L. casei strains showed the same ability. The aromatic profile of fermented juices was characterized by the presence of 82 volatile compounds pertaining to different classes: alcohols, terpenes and norisoprenoids, organic acids, ketones and esters. Elderberry juice fermented with L. plantarum strains showed an increase of total volatile compounds after 48h while the juices fermented with L. rhamnosus and L. casei exhibited a larger increase after the storage. The highest concentration of total volatile compounds were observed in EJ fermented with L. plantarum 285 isolated from dairy product. Ketones increased in all fermented juices both after fermentation and storage and the most concentrated were acetoin and diacetyl. The organic acids were also affected by lactic acid fermentation and the most abundant acids detected in fermented juices were acetic acid and isovaleric acid. Hexanol, 3-hexen-1-ol (Z) and 2-hexen-1-ol (E) were positively influenced during dairy lactic acid bacteria strains fermentation. The most represented esters were ethyl acetate, methyl isovalerate, isoamyl isovalerate and methyl salicylate, all correlated with fruit notes. Among terpenes and norisoprenoids, ß-damascenone resulted the main representative with its typical note of elderberry. Furthermore, coupling obtained data with multivariate statistical analyses, as Principal Component Analysis (PCA) and Classification Trees (CT), it was possible to relate the characteristic volatile profile of samples with the different species and strains applied in this study.
Asunto(s)
Fermentación , Microbiología de Alimentos/métodos , Jugos de Frutas y Vegetales/microbiología , Ácido Láctico/metabolismo , Lacticaseibacillus casei/metabolismo , Lacticaseibacillus rhamnosus/metabolismo , Lactobacillus plantarum/metabolismo , Odorantes/análisis , Sambucus/microbiología , Olfato , Compuestos Orgánicos Volátiles/metabolismo , Jugos de Frutas y Vegetales/análisis , Cromatografía de Gases y Espectrometría de Masas , Lacticaseibacillus casei/crecimiento & desarrollo , Lactobacillus plantarum/crecimiento & desarrollo , Lacticaseibacillus rhamnosus/crecimiento & desarrollo , Sambucus/metabolismo , Microextracción en Fase SólidaRESUMEN
The aims of this study were twofold: a) to provide a detailed report on the phenolic composition and antioxidant activity of fresh berries and leaves of Sambucus lanceolata (Madeiran elderberry); b) to study the effects caused by a simulated in vitro digestion on the composition and antioxidant activity of the berries and leaves. Seventy-seven phytochemicals, mainly polyphenols, were identified in the methanol extracts of fresh berries and leaves, with the content of polyphenols higher in berries (27.2mg·g-1 dry extract, DE) than in leaves (25.9mg·g-1 DE). Anthocyanins were dominant in berries, while hydroxycinnamic acids (HCAs) and flavonols were abundant in leaves. Higher antioxidant activities were found in leaves than in berries, using several in vitro assays. After the simulated in vitro digestion, the levels of polyphenols were significantly reduced, in particular those of berries (81.8% decrease). Anthocyanins were the most affected compounds during the simulated digestion. However, despite the significant loss of phenolic compounds during digestion, methanol extracts of digested berries and leaves were still able to scavenge free-radicals. Hence, the consumption of leaves and/or berries of S. lanceolata may help prevent oxidative stress.
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Antioxidantes/metabolismo , Frutas/metabolismo , Extractos Vegetales/metabolismo , Hojas de la Planta/metabolismo , Polifenoles/metabolismo , Sambucus/metabolismo , Animales , Antioxidantes/análisis , Antioxidantes/química , Cromatografía Líquida de Alta Presión , Frutas/química , Técnicas In Vitro , Extractos Vegetales/química , Hojas de la Planta/química , Polifenoles/análisis , Polifenoles/química , PorcinosRESUMEN
Uptake of glucose and fatty acids in skeletal muscle is of interest for type 2 diabetes treatment. The aim was to study glucose and fatty acid uptake in skeletal muscle cells, antioxidant effects, and inhibition of carbohydrate-hydrolyzing enzymes by elderberries. Enhanced glucose and oleic acid uptake in human skeletal muscle cells were observed after treatment with phenolic elderberry extracts, anthocyanins, procyanidins, and their metabolites. The 96% EtOH and the acidified MeOH extracts were highly active. Of the isolated substances, cyanidin-3-glucoside and cyanidin-3-sambubioside showed highest stimulation of uptake. Phloroglucinol aldehyde was most active among the metabolites. Isolated anthocyanins and procyanidins are strong radical scavengers and are good inhibitors of 15-lipoxygenase and moderate inhibitors of xanthine oxidase. As α-amylase and α-glucosidase inhibitors, they are considerably better than the positive control acarbose. The antidiabetic property of elderberry phenolics increases the nutritional value of this plant and indicates potential as functional food against diabetes.
Asunto(s)
Antocianinas/metabolismo , Biflavonoides/metabolismo , Catequina/metabolismo , Ácidos Grasos/metabolismo , Glucosa/metabolismo , Fibras Musculares Esqueléticas/metabolismo , Fenoles/metabolismo , Extractos Vegetales/metabolismo , Proantocianidinas/metabolismo , Sambucus/metabolismo , Adolescente , Adulto , Antocianinas/aislamiento & purificación , Biflavonoides/aislamiento & purificación , Catequina/aislamiento & purificación , Células Cultivadas , Femenino , Frutas/química , Frutas/metabolismo , Humanos , Masculino , Persona de Mediana Edad , Músculos Oculomotores/citología , Músculos Oculomotores/metabolismo , Fenoles/aislamiento & purificación , Extractos Vegetales/aislamiento & purificación , Proantocianidinas/aislamiento & purificación , Sambucus/química , Células Satélite del Músculo Esquelético/metabolismo , Adulto JovenRESUMEN
In order to better understand biological events, lectin-glycoprotein interactions are of interest. The possibility to gather more information than the mere positive or negative response for interactions brought mass spectrometry into the center of many research fields. The presented work shows the potential of a nano-electrospray gas-phase electrophoretic mobility molecular analyzer (nES GEMMA) to detect weak, noncovalent, biospecific interactions besides still unbound glycoproteins and unreacted lectins without prior liquid phase separation. First results for Sambucus nigra agglutinin, concanavalin A, and wheat germ agglutinin and their retained noncovalent interactions with glycoproteins in the gas phase are presented. Electrophoretic mobility diameters (EMDs) were obtained by nES GEMMA for all interaction partners correlating very well with molecular masses determined by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) of the individual molecules. Moreover, EMDs measured for the lectin-glycoprotein complexes were in good accordance with theoretically calculated mass values. Special focus was laid on complex formation for different lectin concentrations and binding specificities to evaluate the method with respect to results obtained in the liquid phase. The latter was addressed by capillary electrophoresis on-a-chip (CE-on-a-chip). Of exceptional interest was the fact that the formed complexes could be sampled according to their size onto nitrocellulose membranes after gas-phase separation. Subsequent immunological investigation further proved that the collected complex actually retained its native structure throughout nES GEMMA analysis and sampling. Graphical Abstract á .
Asunto(s)
Electroforesis por Microchip/instrumentación , Glicoproteínas/metabolismo , Lectinas/metabolismo , Espectrometría de Masa por Ionización de Electrospray/instrumentación , Animales , Concanavalina A/metabolismo , Diseño de Equipo , Humanos , Dispositivos Laboratorio en un Chip , Lectinas de Plantas/metabolismo , Unión Proteica , Proteínas Inactivadoras de Ribosomas/metabolismo , Sambucus/metabolismo , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/instrumentación , Triticum/metabolismo , Aglutininas del Germen de Trigo/metabolismoRESUMEN
The integration of plant metabolomics to support preharvest fruit development studies can provide important insights into the biochemical mechanisms involved and lately support producers on harvesting management. A metabolomic-based strategy for fingerprinting of volatile terpenoids and norisoprenoids from Sambucus nigra L. berries from three cultivars, through ripening, was established. From 42 monoterpenic, 20 sesquiterpenic, and 14 norisoprenoid compounds, 48 compounds are reported for the first time as S. nigra berries components. Chemometric tools revealed that ripening was the factor that influenced more the volatile fraction profile and physicochemical parameters (pH, TS, and TSS), followed by cultivar. For the unripe stages, a higher overall content of the studied metabolites was observed, which gradually decreased over the ripening stages, being consistent for the three cultivars. These trends were mainly ruled by limonene, p-cymene, aromadendrene, ß-caryophyllene, and dihydroedulan, which might therefore be used by producers as an additional simple decision making tool in conjunction with physicochemical parameters.
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Frutas/crecimiento & desarrollo , Norisoprenoides/química , Sambucus/química , Terpenos/química , Frutas/química , Frutas/genética , Frutas/metabolismo , Cromatografía de Gases y Espectrometría de Masas , Metabolómica , Norisoprenoides/metabolismo , Sambucus/genética , Sambucus/crecimiento & desarrollo , Sambucus/metabolismo , Terpenos/metabolismo , VolatilizaciónRESUMEN
The aim of this study was to investigate the effect of solid-state fermentation (SSF) by Aspergillus niger on phenolic contents and antioxidant activity in Sambucus nigra L. and Sambucus ebulus L. berry pomaces. The effect of fermentation time on the total fats and major lipid classes (neutral and polar) was also investigated. During the SSF, the extractable phenolics increased with 18.82% for S. ebulus L. and 11.11% for S. nigra L. The levels of antioxidant activity of methanolic extracts were also significantly enhanced. The HPLC-MS analysis indicated that the cyanidin 3-sambubioside-5-glucoside is the major phenolic compound in both fermented Sambucus fruit residues. In the early stages of fungal growth, the extracted oils (with TAGs as major lipid fraction) increased with 12% for S. nigra L. and 10.50% for S. ebulus L. The GC-MS analysis showed that the SSF resulted in a slight increase of the linoleic and oleic acids level.
Asunto(s)
Antioxidantes/análisis , Aspergillus niger/metabolismo , Fermentación , Lípidos/análisis , Fenoles/análisis , Sambucus/química , Ácidos Grasos/análisis , Frutas/química , Frutas/microbiología , Ácidos Linoleicos/análisis , Ácido Oléico/análisis , Extractos Vegetales/química , Aceites de Plantas/análisis , Aceites de Plantas/química , Sambucus/metabolismo , Sambucus/microbiología , Sambucus nigra/química , Sambucus nigra/metabolismo , Sambucus nigra/microbiología , Triglicéridos/análisisRESUMEN
The oil content and fatty acid composition of total lipids (TLs) and main lipid classes (NLs- neutral and PLs- polar lipids) in seeds of two wild Sambucus species (S. nigra and S. ebulus) from Transylvania (Romania) were determined by capillary gas chromatography (GC-MS). In addition, the positional distribution of fatty acids in seed triacylglycerols (TAGs) was determined by hydrolysis with pancreatic lipase. The seeds were found to be rich in fat (22.40-24.90 g/100g) with high amounts of polyunsaturated fatty acids (PUFAs) ranging from 68.96% (S. ebulus) to 75.15% (S. nigra). High ratios of PUFAs/SFAs (saturated fatty acids), ranging from 7.06 (S. nigra) to 7.64 (S. ebulus), and low ratios of n-6/n-3, ranging from 0.84 (S. nigra) to 1.51 (S. ebulus), were determined in both oils. The lipid classes/subclasses analyzed (PLs, MAGs--monoacylglycerols, DAGs--diacylglycerols, FFAs--free fatty acids, TAGs and SEs--sterol esters) were separated and identified using thin-layer chromatography. The fatty acid compositions of the TAG fractions were practically identical to the profiles of TLs, with the same dominating fatty acids in both analyzed species. SEs and FFAs, were characterized by high proportions of SFAs. The sn-2 position of TAGs was esterified predominantly with linoleic acid (43.56% for S. nigra and 50.41% for S. ebulus).
Asunto(s)
Ácidos Grasos/metabolismo , Aceites de Plantas/metabolismo , Sambucus/metabolismo , Semillas/metabolismo , Triglicéridos/metabolismo , Cromatografía en Capa Delgada , Ácidos Grasos/química , Ácidos Grasos/aislamiento & purificación , Frutas/metabolismo , Cromatografía de Gases y Espectrometría de Masas , Hidrólisis , Especificidad de Órganos , Pancrelipasa/química , Aceites de Plantas/química , Aceites de Plantas/aislamiento & purificación , Triglicéridos/química , Triglicéridos/aislamiento & purificaciónRESUMEN
We have previously reported fish oil induced hyperlipidemia in BioF1B hamsters compared with Golden Syrian (GS) hamsters. Elderberry (Sambucus nigra L.) extract is abundant in anthocyanins and is believed to exert cardioprotective effects primarily by virtue of its hypolipidemic and antioxidant potential. In the current study, high-fat fish oil feeding increased oxidative stress in BioF1B hamsters compared with GS hamsters; this increase was associated with increased levels of omega-3 polyunsaturated fatty acids in plasma and liver. We then investigated whether cosupplementation with anthocyanin-rich elderberry extract would reverse fish oil induced hyperlipidemia and reduce lipid peroxidation in BioF1B hamsters. Plasma and hepatic lipids decreased significantly when hamsters were fed diets containing elderberry extract along with fish oil. Both plasma and liver thiobarbituric acid reactive substances showed significant reductions upon cosupplementation with elderberry extract in fish oil fed BioF1B hamsters. Our findings demonstrate that cosupplementation with elderberry extract reverses hyperlipidemia and lipid peroxidation observed with dietary fish oil alone in BioF1B hamsters.
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Antioxidantes/farmacología , Aceites de Pescado/farmacología , Hiperlipidemias/metabolismo , Estrés Oxidativo/efectos de los fármacos , Extractos Vegetales/farmacología , Sambucus/metabolismo , Animales , Antioxidantes/metabolismo , Cricetinae , Suplementos Dietéticos , Modelos Animales de Enfermedad , Ácidos Grasos Omega-3/metabolismo , Aceites de Pescado/metabolismo , Peroxidación de Lípido/efectos de los fármacos , Hígado/efectos de los fármacos , Hígado/metabolismo , Masculino , Extractos Vegetales/metabolismo , Sustancias Reactivas al Ácido Tiobarbitúrico/metabolismoRESUMEN
Previously, we developed an alpha2-6-sialic acid (Sia)-specific lectin (SRC) starting from an R-type galactose-specific lectin C-terminal domain. However, it showed relatively low affinity because of its monovalency. Here, we engineered a tandem repeat construct (SRC2) showing substantial affinity for alpha2,6-sialylated N-glycans (in the order of 10(-6)M in K(d)), almost comparable to a natural alpha2-6Sia-specific lectin from Sambucus sieboldiana (SSA). Notably, its binding to branched N-glycans was found to be more selective than SSA. Nevertheless, SRC2 showed no apparent hemagglutinating activity, while it exerted strong erythrocyte-binding activity. This unique feature will help flow cytometry analysis, where usual lectins including SSA agglutinate cells. Some other biochemical properties investigated for SRC2, e.g., high productivity in bacteria and easy release of captured glycoproteins with lactose have demonstrated versatility of this mutant protein as a powerful tool for sialoglycomics.
Asunto(s)
Lectinas/metabolismo , Lectinas de Plantas/genética , Lectinas de Plantas/metabolismo , Ingeniería de Proteínas , Línea Celular , Glicoproteínas/química , Glicoproteínas/metabolismo , Humanos , Lectinas/química , Lectinas/genética , Ácido N-Acetilneuramínico/química , Ácido N-Acetilneuramínico/metabolismo , Lectinas de Plantas/química , Unión Proteica , Sambucus/química , Sambucus/metabolismo , Secuencias Repetidas en Tándem/genéticaRESUMEN
Increased sialylation of cell surface glycoconjugates is among the key molecular changes associated with malignant transformation and cancer progression. We investigated significance of linkage-specific sialylation changes in oral carcinogenesis. Tissue and serum levels of total sialic acid (TSA), linkage-specific sialyltransferases (ST) and sialoproteins were analyzed from patients with oral precancerous conditions (OPC) and oral cancer as well as the post-treatment follow-up blood samples of oral cancer patients. TSA levels were measured using a spectrophotometric method. The linkage-specific lectins, Sambusus nigra (SNA) and Maackia amurensis (MAM) detects alpha 2-6- and alpha 2-3-linked sialic acid, respectively, were used to analyze ST activity and sialoproteins. Malignant tissues showed significantly higher levels of TSA, reactivity of SNA and MAM, and alpha 2,3-ST activity compared to the adjacent normal tissues. alpha 2,6-ST was also higher in malignant tissues. Similarly, the marker levels were higher in precancerous tissues than their adjacent normal tissues. Serum levels of TSA, TSA/ total proteins, alpha 2-6-sialoproteins and alpha 2,6-ST were markedly increased in untreated oral cancer patients compared to the controls and OPC as well as responder (CR) patients. Serum levels of the markers were higher or comparable between untreated oral cancer patients and non-responders (NR). Serum levels of alpha 2-3-sialylation were elevated in non-responders compared with the responders. Further, the observed sialylation changes in tissue and serum were found to be associated with various clinicopathological features and disease progression. Thus, the data suggest potential utility of sialylation markers in early detection, prognostication and treatment monitoring of oral cancer.
Asunto(s)
Neoplasias de la Boca/metabolismo , Ácido N-Acetilneuramínico/metabolismo , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Femenino , Estudios de Seguimiento , Glicoproteínas/metabolismo , Humanos , Maackia/metabolismo , Masculino , Persona de Mediana Edad , Neoplasias de la Boca/patología , Estadificación de Neoplasias , Lectinas de Plantas/metabolismo , Sambucus/metabolismo , Sialiltransferasas/metabolismo , Especificidad por SustratoRESUMEN
Young shoots of Sambucus ebulus L. contain a monomeric d-galactose binding lectin (SELlm), which disappears upon shoot development, and was previously undetected since it co-purifies with the non-toxic type 2 ribosome-inactivating protein ebulin l and the dimeric lectin SELld. Molecular cloning of cDNA coding for SELlm and mass spectrometry analysis revealed a protein with a molecular mass of 34,239 Da, which displays 80%, 77% and 45% of amino acid sequence identity with the ebulin l-B chain, SELld and ricin-B chain, respectively. Furthermore, the cloned precursor, with respect to the ebulin l precursor is truncated and contains the signal peptide, a piece of the A chain, a piece of the connecting peptide and the B chain. Further processing yields the lectin protein, which contains only the B chain. Despite the fact that SELlm displays the same d-galactose-binding sites than ricin, it was found that the lectin has different binding properties to D-galactose-containing matrix than ricin. Notably, and unlike ricin, the binding of SELlm and other Sambucus lectins to such matrix was maximum in range of 0-10 degrees C and abolished at 20 degrees C.
Asunto(s)
Galectinas/metabolismo , Lectinas de Plantas/metabolismo , Brotes de la Planta/metabolismo , Sambucus/metabolismo , Secuencia de Aminoácidos , Cromatografía de Afinidad , Simulación por Computador , Galectinas/química , Galectinas/genética , Modelos Genéticos , Modelos Moleculares , Datos de Secuencia Molecular , Hojas de la Planta/genética , Hojas de la Planta/metabolismo , Lectinas de Plantas/química , Lectinas de Plantas/genética , Brotes de la Planta/genética , Unión Proteica , Estructura Secundaria de Proteína , Ricina/metabolismo , Sambucus/genética , Homología de Secuencia de Aminoácido , Espectrometría de Masa por Láser de Matriz Asistida de Ionización DesorciónRESUMEN
Methanol extract of fruits, leaves and roots of Sambucus ebulus were investigated for antiinflammatory activity in rats (successively, after hexane and ethyl acetate extractions). Nearly all extracts produced statistically significant inhibition of edema induced by carrageenan at all doses when compared to the control groups. Anti-inflammatory effect was generally dose-dependent. The highest activity showed in fruits and leaves that at 600 mg kg(-1) i.p. inhibited 86 and 71% inflammation respectively (76% for diclofenac at 100 mg kg(-1) i.p.). No extracts exhibit any toxicity up to 2 g kg(-1) body weights intraperitoneally in mice. Ethyl acetate extract were withdrawn because of severe nociceptive response in rats. This extract showed severe toxicity (in particular, severe liver abscess) in all mice at all tested doses (125-1500 mg kg(-1) i.p.).
Asunto(s)
Sambucus/metabolismo , Animales , Antiinflamatorios/farmacología , Carragenina/farmacología , Relación Dosis-Respuesta a Droga , Concentración de Iones de Hidrógeno , Inflamación , Masculino , Metanol/farmacología , Ratones , Extractos Vegetales/análisis , Extractos Vegetales/farmacología , Hojas de la Planta , Raíces de Plantas/efectos de los fármacos , Ratas , Ratas WistarRESUMEN
The response of cyanide metabolism rates of two woody plants to changes in temperature is investigated. Detached leaves (1.0 g fresh weight) from weeping willow (Salix babylonica L.) and Chinese elder (Sambucus chinensis Lindl.) were kept in glass vessels with 100ml of aqueous solution spiked with potassium cyanide for a maximum of 28 h. Ten different temperatures were used ranging from 11 degrees C to 32 degrees C. The disappearance of aqueous cyanide was analyzed spectrophotometrically. The cyanide removal rate of Chinese elder was higher than that of weeping willow at all temperatures. The highest cyanide removal rate for Chinese elder was found at 30 degrees C with a value of 12.6 mg CN kg(-1) h(-1), whereas the highest value of the weeping willow was 9.72 mg CN kg(-1) h(-1) at 32 degrees C. The temperature coefficient values, Q10, which are the ratio of removal rates at a 10 degree difference, were determined for Chinese elder and weeping willow to 1.84 and 2.09, respectively, indicating that the cyanide removal rate of weeping willow was much more susceptible to changes in temperature than that of the Chinese elder. In conclusion, changes in temperature have a substantial influence on the removal rate of cyanide by plants.
Asunto(s)
Hojas de la Planta/metabolismo , Cianuro de Potasio/metabolismo , Salix/metabolismo , Sambucus/metabolismo , Temperatura , Biodegradación Ambiental , Especificidad de la Especie , EspectrofotometríaRESUMEN
BACKGROUND: In a recent pilot study, the intake of elderberry juice resulted in a significant decrease in serum cholesterol concentrations and an increase in low-density lipoprotein (LDL) stability. This study was designed to verify the preliminary results. OBJECTIVE: We investigated the impact of elderberry juice on cholesterol and triglyceride concentrations as well as antioxidant status in a cohort of young volunteers. DESIGN: Study A: The randomized, placebo-controlled trial for studying the effect of anthocyanes on lipid and antioxidant status, 34 subjects took capsules with 400 mg spray-dried powder containing 10% anthocyanes t.i.d. equivalent to 5 ml elderberry juice for 2 weeks. A subgroup of 14 subjects continued for an additional week to test for resistance to oxidation of LDL. Study B: To investigate the short-term effects on serum lipid concentrations, six subjects took a single dose of 50 ml of elderberry juice (equivalent to 10 capsules) along with a high-fat breakfast. RESULTS: In the placebo-controlled study, there was only a small, statistically not significant change in cholesterol concentrations in the elderberry group (from 199 to 190 mg/dl) compared to the placebo group (from 192 to 196 mg/dl). The resistance to copper-induced oxidation of LDL did not change within 3 weeks. In the single-dose experiment increases in postprandial triglyceride concentrations were not significantly different when the six subjects were investigated with and without elderberry juice. CONCLUSIONS: Elderberry spray-dried extract at a low dose exerts a minor effect on serum lipids and antioxidative capacity. Higher, but nutritionally relevant doses might significantly reduce postprandial serum lipids.