RESUMEN
The ultrafast internal conversion and intersystem crossing dynamics of 2-thiouracil (2TU) and 2-thiothymine (2TT) are studied using time-resolved photoelectron spectroscopy to investigate the effect of methylation on the deactivation mechanism. Like other thiobases, the triplet manifold is populated with high quantum yields via the lowest singlet excited state, which is dark in absorption. This study focuses on the lowest triplet state and the role of two minima, with sulfur-out-of-plane and slightly boat-like geometries, in the intersystem crossing dynamics back to the ground state.
Asunto(s)
Tiouracilo , Timina , Espectroscopía de Fotoelectrones , Tiouracilo/química , Metilación , Timina/químicaRESUMEN
In this paper, we report X-ray absorption and core-level electron spectra of the nucleobase derivative 2-thiouracil at the sulfur L1- and L2,3-edges. We used soft X-rays from the free-electron laser FLASH2 for the excitation of isolated molecules and dispersed the outgoing electrons with a magnetic bottle spectrometer. We identified photoelectrons from the 2p core orbital, accompanied by an electron correlation satellite, as well as resonant and non-resonant Coster-Kronig and Auger-Meitner emission at the L1- and L2,3-edges, respectively. We used the electron yield to construct X-ray absorption spectra at the two edges. The experimental data obtained are put in the context of the literature currently available on sulfur core-level and 2-thiouracil spectroscopy.
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Rayos Láser , Azufre/química , Tiouracilo/química , Electrones , Espectroscopía de FotoelectronesRESUMEN
In an effort to discover potent anticancer agents, 2-thiouracil-5-sulfonamides derivatives were designed and synthesized. The cytotoxic activity of all synthesized compounds was investigated against four human cancer cell lines viz A-2780 (ovarian), HT-29 (colon), MCF-7 (breast), and HepG2 (liver). Compounds 6b,d-g, and 7b showed promising anticancer activity and significant inhibition of CDK2A. Moreover, they were all safe when tested on WI38 normal cells with high selectivity index for cancer cells. Flow cytometric analysis for the most active compound 6e displayed induction of cell growth arrest at G1/S phase (A-2780 cells), S phase (HT-29 and MCF-7 cells), and G2/M phase (HepG2 cells) and stimulated the apoptotic death of all cancer cells. Moreover, 6e was able to cause cycle arrest indirectly through enhanced expression of cell cycle inhibitors p21 and p27. Finally, molecular docking of compound 6e endorsed its proper binding to CDK2A, which clarifies its potent anticancer activity.
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Antineoplásicos/farmacología , Puntos de Control del Ciclo Celular , Quinasa 2 Dependiente de la Ciclina/antagonistas & inhibidores , Neoplasias/tratamiento farmacológico , Inhibidores de Proteínas Quinasas/farmacología , Sulfonamidas/química , Tiouracilo/química , Antineoplásicos/química , Apoptosis , Proliferación Celular , Diseño de Fármacos , Humanos , Simulación del Acoplamiento Molecular , Estructura Molecular , Neoplasias/enzimología , Neoplasias/patología , Inhibidores de Proteínas Quinasas/química , Relación Estructura-Actividad , Células Tumorales CultivadasRESUMEN
Laser photodissociation spectroscopy (3.1-5.7 eV) has been applied to iodide complexes of the non-native nucleobases, 2-thiouracil (2-TU), 4-thiouracil (4-TU) and 2,4-thiouracil (2,4-TU), to probe the excited states and intracluster electron transfer as a function of sulphur atom substitution. Photodepletion is strong for all clusters (I-·2-TU, I-·4-TU and I-·2,4-TU) and is dominated by electron detachment processes. For I-·4-TU and I-·2,4-TU, photodecay is accompanied by formation of the respective molecular anions, 4-TU- and 2,4-TU-, behaviour that is not found for other nucleobases. Notably, the I-·2TU complex does not fragment with formation of its molecular anion. We attribute the novel formation of 4-TU- and 2,4-TU- to the fact that these valence anions are significantly more stable than 2-TU-. We observe further similar behaviour for I-·4-TU and I-·2,4-TU relating to the general profile of their photodepletion spectra, since both strongly resemble the intrinsic absorption spectra of the respective uncomplexed thiouracil molecule. This indicates that the nucleobase chromophore excitations are determining the clusters' spectral profile. In contrast, the I-·2-TU photodepletion spectrum is dominated by the electron detachment profile, with the near-threshold dipole-bound excited state being the only distinct spectral feature. We discuss these observations in the context of differences in the dipole moments of the thionucleobases, and their impact on the coupling of nucleobase-centred transitions onto the electron detachment spectrum.
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Electrones , Yoduros/química , Tiouracilo/análogos & derivados , Tiouracilo/química , Yoduros/efectos de la radiación , Estructura Molecular , Análisis Espectral , Tiouracilo/efectos de la radiación , Rayos UltravioletaRESUMEN
Thiouracil and thiocytosine are important heterocyclic pharmacophores having pharmacological diversity. Antitumor and antiviral activity is commonly associated with thiouracil and thiocytosine derivatives, which are well known fragments for adenosine receptor affinity with many associated pharmacological properties. In this respect, 33 novel compounds have been synthesized in two groups: 24 thiouracil derivatives (4a-x) and 9 thiocytosine derivatives (5a-i). Antitumor activity of all the compounds was determined in the U87 MG glioblastoma cell line. Compound 5e showed an anti-proliferative IC50 of 1.56 µM, which is slightly higher activity than cisplatin (1.67 µM). The 11 most active compounds showed no signficant binding to adenosine A1, A2A or A2B receptors at 1 µM. Brain tumors express high amounts of phosphodiesterases. Compounds were tested for PDE4 inhibition, and 5e and 5f showed the best potency (5e: 3.42 µM; 5f: 0.97 µM). Remakably, those compounds were also the most active against U87MG. However, the compounds lacked a cytotoxic effect on the HEK293 healthy cell line, which encourages further investigation.
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Antineoplásicos/farmacología , Fosfodiesterasas de Nucleótidos Cíclicos Tipo 4/metabolismo , Citosina/farmacología , Glioblastoma/tratamiento farmacológico , Inhibidores de Fosfodiesterasa 4/farmacología , Receptores Purinérgicos P1/metabolismo , Tiouracilo/farmacología , Antineoplásicos/síntesis química , Antineoplásicos/química , Sitios de Unión/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Citosina/análogos & derivados , Citosina/química , Relación Dosis-Respuesta a Droga , Ensayos de Selección de Medicamentos Antitumorales , Glioblastoma/metabolismo , Glioblastoma/patología , Humanos , Estructura Molecular , Inhibidores de Fosfodiesterasa 4/síntesis química , Inhibidores de Fosfodiesterasa 4/química , Relación Estructura-Actividad , Tiouracilo/síntesis química , Tiouracilo/químicaRESUMEN
Sulfur- and selenium-modified uridines present in the wobble position of transfer RNAs (tRNAs) play an important role in the precise reading of genetic information and tuning of protein biosynthesis in all three domains of life. Both sulfur and selenium chalcogens functionally operate as key elements of biological molecules involved in the protection of cells against oxidative damage. In this work, 2-thiouracil (S2Ura) and 2-selenouracil (Se2Ura) were treated with hydrogen peroxide at 1:0.5, 1:1, and 1:10 molar ratios and at selected pH values ranging from 5 to 8. It was found that Se2Ura was more prone to oxidation than its sulfur analog, and if reacted with H2O2 at a 1:1 or lower molar ratio, it predominantly produced diselenide Ura-Se-Se-Ura, which spontaneously transformed to a previously unknown Se-containing two-ring compound. Its deselenation furnished the major reaction product, a structure not related to any known biological species. Under the same conditions, only a small amount of S2Ura was oxidized to form Ura-SO2H and uracil (Ura). In contrast, 10-fold excess hydrogen peroxide converted Se2Ura and S2Ura into corresponding Ura-SeOnH and Ura-SOnH intermediates, which decomposed with the release of selenium and sulfur oxide(s) to yield Ura as either a predominant or exclusive product, respectively. Our results confirmed significantly different oxidation pathways of 2-selenouracil and 2-thiouracil.
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Tiouracilo/química , Uracilo/análogos & derivados , Peróxido de Hidrógeno/química , Oxidación-Reducción , Uracilo/químicaRESUMEN
We present the first study to measure the dissociative photochemistry of 2-thiouracil (2-TU), an important nucleobase analogue with applications in molecular biology and pharmacology. Laser photodissociation spectroscopy is applied to the deprotonated and protonated forms of 2-TU, which are produced in the gas-phase using electrospray ionization mass spectrometry. Our results show that the deprotonated form of 2-thiouracil ([2-TU-H]-) decays predominantly by electron ejection and hence concomitant production of the [2-TU-H]· free-radical species, following photoexcitation across the UVA-UVC region. Thiocyanate (SCN-) and a m/z 93 fragment ion are also observed as photodecay products of [2-TU-H]- but at very low intensities. Photoexcitation of protonated 2-thiouracil ([2-TU·H]+) across the same UVA-UVC spectral region produces the m/z 96 cationic fragment as the major photofragment. This ion corresponds to ejection of an HS· radical from the precursor ion and is determined to be a product of direct excited state decay. Fragment ions associated with decay of the hot ground state (i.e., the ions we would expect to observe if 2-thiouracil was behaving like UV-dissipating uracil) are observed as much more minor products. This behaviour is consistent with enhanced intersystem crossing to triplet excited states compared to internal conversion back to the ground state. These are the first experiments to probe the effect of protonation/deprotonation on thionucleobase photochemistry, and hence explore the effect of pH at a molecular level on their photophysical properties.
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Tiouracilo/química , Electrones , Radicales Libres/química , Iones/química , Fotoquímica/métodos , Análisis Espectral/métodosRESUMEN
Serinol nucleic acid (SNA) is a promising candidate for nucleic acid-based molecular probes and drugs due to its high affinity for RNA. Our previous work revealed that incorporation of 2,6-diaminpurine (D), which can form three hydrogen bonds with uracil, into SNA increases the melting temperature of SNA-RNA duplexes. However, D incorporation into short self-complementary regions of SNA promoted self-dimerization and hindered hybridization with RNA. Here we synthesized a SNA monomer of 2-thiouracil (sU), which was expected to inhibit base pairing with D by steric hindrance between sulfur and the amino group. To prepare the SNA containing D and sU in high yield, we customized the protecting groups on D and sU monomers that can be readily deprotected under acidic conditions. Incorporation of D and sU into SNA facilitated stable duplex formation with target RNA by suppressing the self-hybridization of SNA and increasing the stability of the heteroduplex of SNA and its complementary RNA. Our results have important implications for the development of SNA-based probes and nucleic acid drugs.
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2-Aminopurina/análogos & derivados , Oligonucleótidos/química , Propanolaminas/química , Glicoles de Propileno/química , ARN/química , Tiouracilo/química , 2-Aminopurina/química , Emparejamiento Base , Enlace de Hidrógeno , Hibridación de Ácido Nucleico , Oligonucleótidos/síntesis química , Oligonucleótidos/genética , Transición de Fase , ARN/genética , Temperatura de TransiciónRESUMEN
The adsorption of Cu(II) ions by biochar fibres prior and after modification with 2-thiouracil on real and artificial samples has been studied by batch-type adsorption experiments, FTIR and XPS spectroscopy and competition reactions using U(VI) ions as competitor cations. The experimental data of the artificial samples clearly show that the modified material presents extraordinary higher affinity for Cu(II) ions even in the acidic pH range, the spectroscopic data indicate the formation of inner-sphere complexes and the competition reactions significantly higher selectivity of the 2-thiouracil modified biochar fibres for Cu(II). The 2-thiouracil-modified biochar fibres have been successfully applied to acid mine drainage (AMD) samples regarding the selective separation of Cu(II) ions from "real" samples. Regarding the desorption of copper from the biochar surface, although 100% copper recovery was achieved by eluting the metal ion using 1â¯M HNO3, the deterioration of the modified biochar fibers due to extensive 2-thiouracil release from the biochar surface limits the applicability of the present adsorbent in routine and large-scale applications.
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Cobre/química , Tiouracilo/química , Uranio/química , Adsorción , Carbón Orgánico , Espectroscopía de Fotoelectrones , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
Oxidative damage to 2-thiouracil (2-TU) by hydroxyl (â¢OH) and azide (âN3) radicals produces various primary reactive intermediates. Their optical absorption spectra and kinetic characteristics were studied by pulse radiolysis with UV-vis spectrophotometric and conductivity detection and by time-dependent density functional theory (TD-DFT) method. The transient absorption spectra recorded in the reactions of â¢OH with 2-TU depend on the concentration of 2-TU, however, only slightly on pH. At low concentrations, they are characterized by a broad absorption band with a weakly pronounced maxima located at λ = 325, 340 and 385 nm, whereas for high concentrations, they are dominated by an absorption band with λmax ≈ 425 nm. Based on calculations using TD-DFT method, the transient absorption spectra at low concentration of 2-TU were assigned to the âOH-adducts to the double bond at C5 and C6 carbon atoms (3â, 4â) and 2c-3e bonded âOH adduct to sulfur atom (1 âOH) and at high concentration of 2-TU also to the dimeric 2c-3e S-S-bonded radical in neutral form (2â). The dimeric radical (2â) is formed in the reaction of thiyl-type radical (6â) with 2-TU and both radicals are in an equilibrium with Keq = 4.2 × 103 M-1. Similar equilibrium (with Keq = 4.3 × 103 M-1) was found for pH above the pKa of 2-TU which involves admittedly the same radical (6â) but with the dimeric 2c-3e S-S bonded radical in anionic form (2â-). In turn, âN3-induced oxidation of 2-TU occurs via radical cation with maximum spin location on the sulfur atom which subsequently undergoes deprotonation at N1 atom leading again to thiyl-type radical (6â). This radical is a direct precursor of dimeric radical (2â).
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Electrones , Oxidación-Reducción/efectos de la radiación , Radiación Ionizante , Tiouracilo/química , Teoría Funcional de la Densidad , Radicales Libres/química , Concentración de Iones de Hidrógeno , Radical Hidroxilo/química , Cinética , Radiólisis de Impulso , Análisis EspectralRESUMEN
The nucleotide analogue, 4-thiouracil (4tU), is readily taken up by cells and incorporated into RNA as it is transcribed in vivo, allowing isolation of the RNA produced during a brief period of labelling. This is done by attaching a biotin moiety to the incorporated thio group and affinity purifying, using streptavidin coated beads. Achieving a good yield of pure, newly synthesized RNA that is free of pre-existing RNA makes shorter labelling times possible and permits increased temporal resolution in kinetic studies. This is a protocol for very specific, high yield purification of newly synthesized RNA. The protocol presented here describes how RNA is extracted from the yeast Saccharomyces cerevisiae. However, the protocol for purification of thiolated RNA from total RNA should be effective using RNA from any organism once it has been extracted from the cells. The purified RNA is suitable for analysis by many widely used techniques, such as reverse transcriptase-qPCR, RNA-seq and SLAM-seq. The specificity, sensitivity and flexibility of this technique allow unparalleled insights into RNA metabolism.
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ARN/química , ARN/metabolismo , Tiouracilo/análogos & derivados , Biotina/metabolismo , Cromatografía de Afinidad , Regulación de la Expresión Génica , Regulación Fúngica de la Expresión Génica , Cinética , ARN/genética , Saccharomyces cerevisiae/metabolismo , Proteínas de Saccharomyces cerevisiae , Estreptavidina , Tiouracilo/químicaRESUMEN
Au nanoclusters (Au NCs) with a unique size effect on the antibacterial performance provide a promising nanoprobe for developing an efficient nanomedicine. However, little progress has been made owing to the low quantum yield and poor stability of Au NCs. In this work, protamine (Prot) functionalized Au NCs (Prot/MTU-Au NCs) with high stability were achieved through a simple mixing with 6-methyl-2-thiouracil-capped Au NCs (MTU-Au NCs) due to the hydrogen bonding between 5-methyl-2-thiouracil (MTU) and the guanidine groups from Prot. Interestingly, a distinctly enhanced photoluminescence from Prot/MTU-Au NCs (ca. 28-fold) was observed due to the formation of rigid host-guest assemblies. We inferred that the cross-linked structure and supramolecular hydrogen bonds both contributed to the fluorescence enhancement and stability. The extra small size of the NCs and the efficient antibacterial capability from the capping shell of Prot encouraged us to probe its antibacterial performance systemically. It was found that the Prot/MTU-Au NCs with highly stable loading of positively charged antibacterial reagents were likely to penetrate into the bacteria and thus enhance the ability to kill both Gram-negative bacteria (Escherichia coli) and Gram-positive bacteria (multiple-resistant Staphylococcus aureus). The synergetic effect between the unique size and the capping layers enabled the minimal inhibitory concentration of the as-derived Prot/MTU-Au NCs reduced by â¼100-fold compared to that with individual Au nanoparticle. The antibacterial mechanism further revealed that membrane injury occurred and reactive oxygen species were generated after the incubation of the bacteria with Prot/MTU-Au NCs. Moreover, the highly luminescent fluorescence and positive surface charge of Prot/MTU-Au NCs could image the bacteria easily, which held great potential for imaging-guided antibacterial platform.
Asunto(s)
Antibacterianos/farmacología , Oro/química , Imagenología Tridimensional , Nanopartículas del Metal/química , Escherichia coli/efectos de los fármacos , Escherichia coli/crecimiento & desarrollo , Humanos , Nanopartículas del Metal/ultraestructura , Pruebas de Sensibilidad Microbiana , Protaminas/química , Especies Reactivas de Oxígeno/metabolismo , Staphylococcus aureus/efectos de los fármacos , Staphylococcus aureus/crecimiento & desarrollo , Tiouracilo/análogos & derivados , Tiouracilo/químicaRESUMEN
The molecular structure of 2-thiouracil, 4-thiouracil and 2,4-dithiouracil was analyzed under the effect of the first and second hydration shell by using the B3LYP density functional (DFT) method, and the results were compared to those obtained for the uracil molecule. A slight difference in the water distribution appears in these molecules. On the hydration of these molecules several trends in bond lengths and atomic charges were established. The ring in uracil molecule appears easier to be deformed and adapted to different environments as compared to that when it is thio-substituted. Molecular docking calculations of 2-thiouracil against three different pathogens: Bacillus subtilis, Escherichia coli and Candida albicans were carried out. Docking calculations of 2,4-dithiouracil ligand with various targeted proteins were also performed. Different DNA: RNA hybrid microhelixes with uridine, 2-thiouridine, 4-thiouridine and 2,4-dithiouridine nucleosides were optimized in a simple model with three nucleotide base pairs. Two main types of microhelixes were analyzed in detail depending on the intramolecular H-bond of the 2'-OH group. The weaker Watson-Crick (WC) base pair formed with thio-substituted uracil than with unsubstituted ones slightly deforms the helical and backbone parameters, especially with 2,4-dithiouridine. However, the thio-substitution significantly increases the dipole moment of the A-type microhelixes, as well as the rise and propeller twist parameters.
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Antiinfecciosos/química , Simulación del Acoplamiento Molecular , Tiouracilo/química , Animales , Antiinfecciosos/farmacología , Bacillus subtilis/efectos de los fármacos , Candida albicans/efectos de los fármacos , ADN/química , Escherichia coli/efectos de los fármacos , Humanos , Interacciones Hidrofóbicas e Hidrofílicas , Unión Proteica , ARN/química , Tiouracilo/farmacologíaRESUMEN
Analysis of cell-type-specific transcriptomes is vital for understanding the biology of tissues and organs in the context of multicellular organisms. In this Protocol Extension, we combine a previously developed cell-type-specific metabolic RNA labeling method (thiouracil (TU) tagging) and a pipeline to detect the labeled transcripts by a novel RNA sequencing (RNA-seq) method, SLAMseq (thiol (SH)-linked alkylation for the metabolic sequencing of RNA). By injecting a uracil analog, 4-thiouracil, into transgenic mice that express cell-type-specific uracil phosphoribosyltransferase (UPRT), an enzyme required for 4-thiouracil incorporation into newly synthesized RNA, only cells expressing UPRT synthesize thiol-containing RNA. Total RNA isolated from a tissue of interest is then sequenced with SLAMseq, which introduces thymine to cytosine (T>C) conversions at the sites of the incorporated 4-thiouracil. The resulting sequencing reads are then mapped with the T>C-aware alignment software, SLAM-DUNK, which allows mapping of reads containing T>C mismatches. The number of T>C conversions per transcript is further analyzed to identify which transcripts are synthesized in the UPRT-expressing cells. Thus, our method, SLAM-ITseq (SLAMseq in tissue), enables cell-specific transcriptomics without laborious FACS-based cell sorting or biochemical isolation of the labeled transcripts used in TU tagging. In the murine tissues we assessed previously, this method identified ~5,000 genes that are expressed in a cell type of interest from the total RNA pool from the tissue. Any laboratory with access to a high-throughput sequencer and high-power computing can adapt this protocol with ease, and the entire pipeline can be completed in <5 d.
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Perfilación de la Expresión Génica/métodos , Especificidad de Órganos/genética , Análisis de Secuencia de ARN/métodos , Transcriptoma/genética , Animales , Femenino , Secuenciación de Nucleótidos de Alto Rendimiento , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Transgénicos , Pentosiltransferasa/genética , Pentosiltransferasa/metabolismo , Tiouracilo/análogos & derivados , Tiouracilo/química , Tiouracilo/metabolismoRESUMEN
The RNA binding proteome (RBPome) was previously investigated using UV crosslinking and purification of poly(A)-associated proteins. However, most cellular transcripts are not polyadenylated. We therefore developed total RNA-associated protein purification (TRAPP) based on 254 nm UV crosslinking and purification of all RNA-protein complexes using silica beads. In a variant approach (PAR-TRAPP), RNAs were labelled with 4-thiouracil prior to 350 nm crosslinking. PAR-TRAPP in yeast identified hundreds of RNA binding proteins, strongly enriched for canonical RBPs. In comparison, TRAPP identified many more proteins not expected to bind RNA, and this correlated strongly with protein abundance. Comparing TRAPP in yeast and E. coli showed apparent conservation of RNA binding by metabolic enzymes. Illustrating the value of total RBP purification, we discovered that the glycolytic enzyme enolase interacts with tRNAs. Exploiting PAR-TRAPP to determine the effects of brief exposure to weak acid stress revealed specific changes in late 60S ribosome biogenesis. Furthermore, we identified the precise sites of crosslinking for hundreds of RNA-peptide conjugates, using iTRAPP, providing insights into potential regulation. We conclude that TRAPP is a widely applicable tool for RBPome characterization.
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Proteínas de Unión al ARN/metabolismo , ARN/metabolismo , Ribonucleoproteínas/aislamiento & purificación , Tiouracilo/análogos & derivados , Reactivos de Enlaces Cruzados/química , Escherichia coli/genética , Escherichia coli/metabolismo , Proteínas de Escherichia coli/metabolismo , ARN/química , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Tiouracilo/químicaRESUMEN
Thiouracil, 2-sulfanylidene-1H-pyrimidin-4-one, has been used as anti-thyroid, coronary vasodilator, and in congestive heart failure. It was found to cause agranulocytosis and it is suspected to be teratogenic and carcinogenic. Owing to its high frequency of adverse reactions, especially agranulocytosis, its use was abandoned in favor of other, less toxic drugs, such as propylthiouracil and methimazole. Thiouracil refers both to a specific molecule consisting of a sulfated uracil and a family of molecules based upon the structure. An important member of this family is propylthiouracil, which is a thiourea antithyroid drug that acts by blocking the production of thyroid hormones; it also inhibits the peripheral deiodination of thyroxine to tri-iodothyronine. This profile is prepared to discuss and explain physical and chemical properties, proprietary and nonproprietary names of thiouracil and propylthiouracil. It also includes uses and applications, methods of preparation, thermal and spectral behavior and methods of analysis. In addition, metabolism, excretion and pharmacology of propylthiouracil are also discussed.
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Antitiroideos/farmacología , Tiouracilo/farmacología , Antitiroideos/química , Metimazol , Propiltiouracilo , Tiouracilo/química , TiroxinaRESUMEN
Many open questions in RNA biology relate to the kinetics of gene expression and the impact of RNA binding regulatory factors on processing or decay rates of particular transcripts. Steady state measurements of RNA abundance obtained from RNA-seq approaches are not able to separate the effects of transcription from those of RNA decay in the overall abundance of any given transcript, instead only giving information on the (presumed steady-state) abundances of transcripts. Through the combination of metabolic labeling and high-throughput sequencing, several groups have been able to measure both transcription rates and decay rates of the entire transcriptome of an organism in a single experiment. This review focuses on the methodology used to specifically measure RNA decay at a global level. By comparing and contrasting approaches and describing the experimental protocols in a modular manner, we intend to provide both experienced and new researchers to the field the ability to combine aspects of various protocols to fit the unique needs of biological questions not addressed by current methods.
Asunto(s)
Química Clic/métodos , Secuenciación de Nucleótidos de Alto Rendimiento/métodos , ARN Mensajero/metabolismo , Coloración y Etiquetado/métodos , Transcriptoma , Animales , Biotina/análogos & derivados , Biotina/química , Bromouracilo/análogos & derivados , Línea Celular , Humanos , Estabilidad del ARN , ARN Mensajero/genética , Tiouracilo/análogos & derivados , Tiouracilo/química , Tiouracilo/metabolismo , Tiouridina/química , Tiouridina/metabolismo , Uracilo/análogos & derivados , Uracilo/química , Uracilo/metabolismo , Uridina/análogos & derivados , Uridina/química , Uridina/metabolismoRESUMEN
We report time-dependent photoelectron spectra recorded with a single-photon ionization setup and extensive simulations of the same spectra for the excited-state dynamics of 2-thiouracil (2TU) in the gas phase. We find that single-photon ionization produces very similar results as two-photon ionization, showing that the probe process does not have a strong influence on the measured dynamics. The good agreement between the single-photon ionization experiments and the simulations shows that the norms of Dyson orbitals allow for qualitatively describing the ionization probabilities of 2TU. This reasonable performance of Dyson norms is attributed to the particular electronic structure of 2TU, where all important neutral and ionic states involve similar orbital transitions and thus the shape of the Dyson orbitals do not strongly depend on the initial neutral and final ionic state. We argue that similar situations should also occur in other biologically relevant thio-nucleobases, and that the time-resolved photoelectron spectra of these bases could therefore be adequately modeled with the techniques employed here.
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Modelos Químicos , Espectroscopía de Fotoelectrones , Tiouracilo/química , Algoritmos , Fotoquímica , Fotones , TermodinámicaRESUMEN
Hyperthyroidism is the result of uncontrolled overproduction of the thyroid hormones. One of the mostly used antithyroid agents is 6-n-propyl-2-thiouracil (PTU). The previously solved X-ray crystal structure of the PTU bound to mammalian lactoperoxidase (LPO) reveals that the LPO-PTU binding site is basically a hydrophobic channel. There are two hydrophobic side chains directed towards the oxygen atom in the C-4 position of the thiouracil ring. In the current study, the structural activity relationship (SAR) was performed on the thiouracil nucleus of PTU to target these hydrophobic side chains and gain more favorable interactions and, in return, more antithyroid activity. Most of the designed compounds show superiority over PTU in reducing the mean serum T4 levels of hyperthyroid rats by 3% to 60%. In addition, the effect of these compounds on the levels of serum T3 was found to be comparable to the effect of PTU treatment. The designed compounds in this study showed a promising activity profile in reducing levels of thyroid hormones and follow up experiments will be needed to confirm the use of the designed compounds as new potential antithyroid agents.
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Antitiroideos/administración & dosificación , Antitiroideos/síntesis química , Hipertiroidismo/tratamiento farmacológico , Tiouracilo/administración & dosificación , Tiouracilo/síntesis química , Animales , Antitiroideos/química , Antitiroideos/farmacología , Sitios de Unión , Modelos Animales de Enfermedad , Interacciones Hidrofóbicas e Hidrofílicas , Hipertiroidismo/sangre , Lactoperoxidasa/química , Modelos Moleculares , Ratas , Relación Estructura-Actividad , Tiouracilo/química , Tiouracilo/farmacología , Triyodotironina/sangre , Uracilo/análogos & derivados , Uracilo/químicaRESUMEN
Global defects in RNA polymerase II transcription might be overlooked by transcriptomic studies analyzing steady-state RNA. Indeed, the global decrease in mRNA synthesis has been shown to be compensated by a simultaneous decrease in mRNA degradation to restore normal steady-state levels. Hence, the genome-wide quantification of mRNA synthesis, independently from mRNA decay, is the best direct reflection of RNA polymerase II transcriptional activity. Here, we discuss a method using non-perturbing metabolic labeling of nascent RNAs in Saccharomyces cerevisiae (S. cerevisiae). Specifically, the cells are cultured for 6 min with a uracil analog, 4-thiouracil, and the labeled newly transcribed RNAs are purified and quantified to determine the synthesis rates of all individual mRNA. Moreover, using labeled Schizosaccharomyces pombe cells as internal standard allows comparing mRNA synthesis in different S. cerevisiae strains. Using this protocol and fitting the data with a dynamic kinetic model, the corresponding mRNA decay rates can be determined.