RESUMEN
Reversed-phase separation of tocopherols (Ts) and tocotrienols (Ts) using C18 stationary phases results in the coelution of ß and γ positional isomers, leading to identification errors. This study investigates the potential of alternative stationary phase chemistries to effectively resolve tocochromanols, specifically focusing on the critical pair of ß and γ positional isomers. Initial screening of seven different stationary phases (C18, C18-PFP, C30, PFP, 5PYE, πNAP, and RP-Amide) was conducted. Linear solvent strength (LSS) studies were performed to assess the impact of the organic modifier (methanol) and temperature on the chromatographic performance parameters. Five columns were found to be suitable for the tocochromanol separation and two different chromatographical conditions per column were proposed. Elution order of tocochromanols was unique for 5PYE, πNAP and C30 columns in comparison to RP-Amide and PFP. Method development for the quantitative analysis of four tocopherol and four tocotrienol homologues was performed. The optimised method employed the RP-Amide (150 × 4.6 mm, 2.6 µm dp) superficially porous particle column, mobile phase of methanol:water of 92:8, v/v, with a flow rate of 1.0 mL/min, column oven temperature of 40 °C and fluorescence detection (λex 295 nm, λem 330 nm). The analysis run time was 10.5 min with 13.6 MPa back pressure. The method was validated and the obtained LOQs were found to be 1.30-3.13 µg/mL. The method developed was successfully applied for the determination of tocochromanols in twenty samples with unique tocochromanol profiles. Principal component analysis illustrated three distinct groups based on the tocochromanol profile.
Asunto(s)
Cromatografía de Fase Inversa , Tocoferoles , Tocotrienoles , Tocotrienoles/análisis , Tocotrienoles/aislamiento & purificación , Tocoferoles/análisis , Tocoferoles/química , Tocoferoles/aislamiento & purificación , Isomerismo , Cromatografía de Fase Inversa/métodos , Cromatografía Líquida de Alta Presión/métodosRESUMEN
Four undescribed modified tocotrienols, including two monomers, litchinols A (1) and B (2), and two walsurol dimers, δ,δ-walsurol (3) and γ,δ-bi-O-walsurol (4), as well as seven known compounds (5-11) were isolated from the roots of Litchi chinensis. The structures of the undescribed compounds were elucidated based on analyses of spectroscopic data and ECD spectra. All tocotrienol derivatives (1-6) were evaluated for their tyrosinase inhibition activity. Only monomers 1-2 and 5-6 displayed potent inhibitory activity and greater than kojic acid. Kinetic analysis revealed that the representative compound 2 was uncompetitive inhibitor with the inhibition constant value of 5.70 µM.
Asunto(s)
Litchi , Tocotrienoles , Litchi/química , Tocotrienoles/farmacología , Tocotrienoles/análisis , Monofenol Monooxigenasa , Cinética , Frutas/químicaRESUMEN
The tocopherol (T) and tocotrienol (T3) profile were investigated in the present study for four hundred and eighteen plant oil samples, including thirty-one families, eighty-two species, and five cross-species. Fifteen species were dominated by tocotrienols, while sixty-seven - by tocopherols. The mean proportion of γ-T was almost half of the total tocochromanol content (49.3%) in the investigated samples, while α-T constituted to one quarter (25.0%), and the remaining other tocochromanols were present as minor constituents. A strong relationship between the taxonomic plant origin and the presence of the characteristic tocochromanol profile in oils obtained from those plants was noted. This is the first study to demonstrate that not only monocotyledonous, but also dicotyledons families can be rich in tocotrienols. The usefulness of statistical tools - principal component analysis (PCA) and hierarchical cluster analysis (HCA) for plant sample discrimination based on their tocochromanol profile was also shown.
Asunto(s)
Tocoferoles , Tocotrienoles , Humanos , Tocoferoles/análisis , Tocotrienoles/análisis , Aceites de Plantas , Análisis de Componente Principal , Análisis por ConglomeradosRESUMEN
Free and esterified (bound) tocopherols, tocotrienols and other tocochromanol-related compounds, often referred to "tocols", are lipophilic antioxidants of great importance for health. For instance, α-tocopherol is the only tocochromanol with vitamin E activity, while tocotrienols have a positive impact on health and are proposed in the prevention and therapy of so-called modern diseases. Tocopherols, tocotrienols and plastochromanol-8 are the most well-known tocochromanols; in turn, knowledge about tocodienols, tocomonoenols, and other rare tocochromanol-related compounds is limited due to several challenges in analytical chemistry and/or low concentration in plant material. The presence of free, esterified, and non-extractable tocochromanols in plant material as well as their biological function, which may be of great scientific, agricultural and medicinal importance, is also poorly studied. Due to the lack of modern protocols as well as equipment and tools, for instance, techniques suitable for the efficient and simultaneous chromatographical separation of major and minor tocochromanols, the topic requires attention and new solutions, and/or standardization, and proper terminology. This review discusses the advantages and disadvantages of different chromatographic techniques, tools and approaches used for the separation and detection of different tocochromanols in plant material and foodstuffs. Sources of tocochromanols and procedures for obtaining different tocochromanol analytical standards are also described. Finally, future challenges are discussed and perspective green techniques for tocochromanol determination are proposed along with best practice recommendations. The present manuscript aims to present key aspects and protocols related to tocochromanol determination, correct identification, and the interpretation of obtained results.
Asunto(s)
Tocotrienoles , Antioxidantes , Tocoferoles/química , Tocotrienoles/análisis , Vitamina E , alfa-TocoferolRESUMEN
A supercritical fluid chromatography with electrochemical detection system was developed for the simultaneous determination of tocopherol and tocotrienol isomers. The supercritical fluid chromatography with electrochemical detection system was connected with an additional pump to create a flow path to add a supporting electrolyte solution. The supporting electrolyte solution was mixed with a mobile phase in a post-column fashion, enabling the independent control of the separation and detection. After optimization of the measurement conditions, vitamin E isomers and an internal standard substance (2,2,5,7,8-pentamethyl-6-hydroxychroman) were separated within 30 min using a mixture of supercritical carbon dioxide and methanol (99:1, v/v) as a mobile phase and a cyanopropyl column (4.6 mm inner diameter × 250 mm length, 5 µm). For the electrochemical detection, methanol containing 1.0 mol/L ammonium acetate was used as a supporting electrolyte solution, and the applied potential was set at +0.8 V. This analytical method showed good linearity (5-100 µg/mL) and repeatability (less than 2.5% relative standard deviation, n = 6) and was applicable to the determination of tocopherol and tocotrienol isomers in nutrition supplements.
Asunto(s)
Cromatografía con Fluido Supercrítico , Tocotrienoles , Cromatografía Líquida de Alta Presión/métodos , Cromatografía con Fluido Supercrítico/métodos , Electrólitos , Metanol , Tocoferoles , Tocotrienoles/análisis , Vitamina E/análisisRESUMEN
Vitamin E is a group of isoprenoid chromanols with different biological activities. It comprises eight oil-soluble compounds: four tocopherols, namely, α-, ß-, γ-, and δ-tocopherols; and four tocotrienols, namely, α-, ß-, γ, and δ-tocotrienols. Vitamin E isomers are well-known for their antioxidant activity, gene-regulation effects, and anti-inflammatory and nephroprotective properties. Considering that vitamin E is exclusively synthesized by photosynthetic organisms, animals can only acquire it through their diet. Plant-based food is the primary source of vitamin E; hence, oils, nuts, fruits, and vegetables with high contents of vitamin E are mostly consumed after processing, including industrial processes and home-cooking, which involve vitamin E profile and content alteration during their preparation. Accordingly, it is essential to identify the vitamin E content and profile in foodstuff to match daily intake requirements. This review summarizes recent advances in vitamin E chemistry, metabolism and metabolites, current knowledge on their contents and profiles in raw and processed plant foods, and finally, their modern developments in analytical methods.
Asunto(s)
Tocotrienoles , Vitamina E , Animales , Antioxidantes/química , Tecnología de Alimentos , Tocoferoles/química , Tocoferoles/metabolismo , Tocotrienoles/análisis , Tocotrienoles/química , Tocotrienoles/metabolismoRESUMEN
Excessive high fat dietary intake promotes risk of developing non-alcoholic fatty liver disease (NAFLD) and predisposed with oxidative stress. Palm based tocotrienol-rich fraction (TRF) has been reported able to ameliorate oxidative stress but exhibited poor bioavailability. Thus, we investigated whether an enhanced formulation of TRF in combination with palm kernel oil (medium-chain triglycerides) (ETRF) could ameliorate the effect of high-fat diet (HFD) on leptin-deficient male mice. All the animals were divided into HFD only (HFD group), HFD supplemented with ETRF (ETRF group) and HFD supplemented with TRF (TRF group) and HFD supplemented with PKO (PKO group). After 6 weeks, sera were collected for untargeted metabolite profiling using UHPLC-Orbitrap MS. Univariate analysis unveiled alternation in metabolites for bile acids, amino acids, fatty acids, sphingolipids, and alkaloids. Bile acids, lysine, arachidonic acid, and sphingolipids were downregulated while xanthine and hypoxanthine were upregulated in TRF and ETRF group. The regulation of these metabolites suggests that ETRF may promote better fatty acid oxidation, reduce oxidative stress and pro-inflammatory metabolites and acts as anti-inflammatory in fatty liver compared to TRF. Metabolites regulated by ETRF also provide insight of its role in fatty liver. However, further investigation is warranted to identify the mechanisms involved.
Asunto(s)
Arecaceae/química , Biomarcadores/sangre , Dieta Alta en Grasa , Metaboloma , Metabolómica , Tocotrienoles/análisis , Animales , Cromatografía Líquida de Alta Presión , Suplementos Dietéticos , Modelos Animales de Enfermedad , Inmunohistoquímica , Hígado/efectos de los fármacos , Hígado/metabolismo , Hígado/patología , Masculino , Espectrometría de Masas , Metabolómica/métodos , Ratones , Enfermedad del Hígado Graso no Alcohólico/etiología , Enfermedad del Hígado Graso no Alcohólico/metabolismo , Enfermedad del Hígado Graso no Alcohólico/patología , Aceite de PalmaRESUMEN
In this study, we investigated the compositions of vitamin E tocochromanol [tocopherol (Toc) and tocotrienol (T3)] in crude and refined rice bran oil (RBO) produced in Japan and other countries, including Brazil, Thailand, and Vietnam, based on high-performance liquid chromatography analysis. All RBO analyzed contained α-, ß- and γ-Toc and α-, γ- and δ-T3. Japanese crude RBO, although not refined RBO, also contained ß-T3. Furthermore, total Toc contents in both Japanese crude and refined oils were found to be higher than those in the crude and refined RBO from other countries. Total T3 contents in Japanese crude RBO were similar to those in the crude RBO from Brazil and Vietnam. The α-Toc and α-T3 contents in Japanese crude and refined RBO were considerably higher than those in the crude and refined RBO produced in other countries, whereas in contrast, γ-Toc and γ-T3 contents in Japanese crude and refined RBO were lower. Consequently, the ratios of total α-Toc and α-T3 contents to total γ-Toc and γ-T3 contents in Japanese crude and refined RBO (1.75 and 1.91, respectively) were notably higher than those in the crude and refined RBO produced in other countries. Similarly, the ratios of total Toc to total T3 in Japanese crude and refined RBO were higher than those in the crude and refined RBO produced in other countries. These results accordingly indicate that the ratio of total α-Toc and α-T3 contents to γ-Toc and γ-T3 contents could be used as an effective index to discriminate between the RBO produced in Japan and that produced in other countries.
Asunto(s)
Análisis de los Alimentos/métodos , Aceite de Salvado de Arroz/química , Tocoferoles/análisis , Tocotrienoles/análisis , Brasil , Cromatografía Líquida de Alta Presión , Japón , Aceite de Salvado de Arroz/clasificación , Tailandia , VietnamRESUMEN
The aim of this study was to analyse contents of phytosterols, carotenoids, tocochromanols and fatty acid composition in oil from nutlets of linden. Standard determination methods for these compounds were applied using liquid and gas chromatography. In the analysed oils from small and large-leaved lime the dominant acids included linoleic (53.1 and 54.1%), oleic (18.5 and 22.2%), palmitic (15.3 and 11.8%). Linden seed oil was characterised by very high contents of phytosterols (2-2.5 g/100 g). ß-Sitosterol was the dominant phytosterol (80-83%). Additionally squalene was detected at 806 and 607 mg/100 g, respectively, for Tilia cordata and T.platyphyllos. It was found that linden seed oil contains tocopherols (93%) and tocotrienols (7%). In terms of tocochromanol contents this oil is surpassed only by wheat germ oil. In terms of the α-T equivalent these oils contain 95 (Tilia cordata) and 50.6 mg/100 g vitamin E (Tilia platyphyllos).
Asunto(s)
Carotenoides/análisis , Tilia/química , Tocoferoles/análisis , Tocotrienoles/análisis , Cromatografía de Gases , Cromatografía Líquida de Alta Presión , Ácidos Grasos/análisis , Aceites de Plantas/química , Semillas/química , Semillas/metabolismo , Tilia/metabolismoRESUMEN
The consumption of vegetable oil is an important way for the body to obtain tocols. However, the impact of oil types and grades on the tocopherol and tocotrienol contents in vegetable oils is unclear. In this study, nine types of traditional edible oils and ten types of self-produced new types of vegetable oil were used to analyze eight kinds of tocols. The results showed that the oil types exerted a great impact on the tocol content of traditional edible oils. Soybean oils, corn oils, and rapeseed oils all could be well distinguished from sunflower oils. Both sunflower oils and cotton seed oils showed major differences from camellia oils as well as sesame oils. Among them, rice bran oils contained the most abundant types of tocols. New types of oil, especially sacha inchi oil, have provided a new approach to obtaining oils with a high tocol content. Oil refinement leads to the loss of tocols in vegetable oil, and the degree of oil refinement determines the oil grade. However, the oil grade could not imply the final tocol content in oil from market. This study could be beneficial for the oil industry and dietary nutrition.
Asunto(s)
Aceites de Plantas/química , Tocoferoles/análisis , Tocotrienoles/análisisRESUMEN
BACKGROUND: One of the requirements for proper nutrition and maintenance of good health is to supply the body, through diet, with an appropriately increased quantity of bioactive compounds. With this in mind, modern milling and baking industries keep introducing new types of products. The use of such additives as wholegrain flours and bran in baked products provided the basis for research in this paper. METHODS: The aim of the study was to conduct a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour), as well as wheat bran. Additionally, analyses were performed to determine the chemical composition and contents of nutrients, selected bioactive compounds and antioxidant activity. RESULTS: The studied raw materials in commercial cereal differ in their chemical composition. Dehulling of wheat, barley and oat grains significantly contributed to the reduction of minerals, protein and total dietary fiber (TDF) contents, except for the amount of protein in dehulled wheat and oat grains. Oat bran, in contrast to other oat products, was characterized by the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch. The lowest content of minerals was recorded in wheat flour type 550. Thermal processes affect the concentration of tocochromanols in the grain, with tocotrienols being more resistant to hydrothermal treatment than tocopherols. Grain dehulling also significantly decreased the total amount of tocochromanols in relation to the original grains. CONCLUSIONS: Wheat products differ in the contents of their individual components. They are characterized by high contents of tocochromanols, phenolic compounds and water-extractable arabinoxylans, with the exception of white refined wheat flour, which is mainly a source of saccharides. In the case of barley and oat products, the analysis showed no differences between these product groups. It was also shown that dehulling of barley and oat grain causes statistically significant differences in the contents of nutrients and natural antioxidants.
Asunto(s)
Avena/química , Grano Comestible/química , Hordeum/química , Triticum/química , Antioxidantes/análisis , Harina , Manipulación de Alimentos , Calor , Humanos , Minerales/análisis , Nutrientes/análisis , Fenoles/análisis , Tocotrienoles/análisis , Granos Enteros/química , Xilanos/análisisRESUMEN
Tocochromanols are a group of lipid-soluble antioxidants that include tocopherols, tocotrienols and plastochromanol-8. Here, we examined a putative differential accumulation of tocochromanols in photosynthetic and non-photosynthetic tissues (including leaves and whole fruits) of strawberry (Fragaria x ananassa cv. Albion) plants and evaluated their endogenous variations in response to a reiterated water deficit during a vegetative (non-productive) and a fruiting (productive) period. In addition, we evaluated the concentration of tocochromanols in achenes (true fruits) and flesh of strawberries (whole fruits) at the white and full-red stages both under optimal and stress conditions. Results showed that leaves mainly accumulated α-tocopherol, with plastochromanol-8 and γ-tocopherol being present at low amounts. In contrast, whole fruits did not accumulate plastochromanol-8, γ-tocopherol being the major tocochromanol in the achenes (true fruit) and α-tocopherol in the flesh. While α-tocopherol content in leaves increased up to seven-fold after 12 weeks of stress during the fruiting period, it kept unaltered during the vegetative period. Neither plastochromanol-8 nor γ-tocopherol contents increased in leaves of stressed plants. During the fruiting period, γ-tocopherol content increased in whole fruits of stressed plants (most of it being accumulated in the achenes). Among the compounds examined, the flesh of strawberries accumulated α-tocopherol only, both at the white and full-red stages. It is concluded that (i) α-tocopherol is the major tocochromanol in leaves, while γ-tocopherol is the major tocochromanol in achenes (ii) reiterated water deficit promotes the accumulation of α-tocopherol in leaves and γ-tocopherol in fruits, (iii) α-tocopherol not only accumulates in photosynthetic tissues (leaves and whole fruits at green stages), but also in non-photosynthetic tissues (flesh of whole fruits at the white and full-red stages), and (iv) achenes (true fruits) of strawberry plants are an extraordinary rich source of tocopherols.
Asunto(s)
Sequías , Fragaria/química , Tocoferoles/análisis , Tocotrienoles/análisis , Fotosíntesis , Estrés Fisiológico , AguaRESUMEN
BACKGROUND: Black sweet corn as an edible fruit has various nutritional qualities. This study discusses changes in the vitamin C and E, folate, and carotenoid content during black sweet corn maturation, and also the effects of preharvest weather conditions and of related genes in multi-vitamin biosynthesis pathways. RESULTS: Most vitamin levels improved, especially vitamin C and carotenoid levels, while the folate content dropped rapidly. Transcript levels of most genes in folate biosynthesis showed trends that were similar to the content changes. VTC2 and GLDH, which are regulated by light, had high expression levels leading to an increase in ascorbate content during maturation. γ-Tocotrienol is the main vitamin E component, and HGGT, the key gene controlling the synthesis of tocotrienols, had a much higher expression level than other genes. Lutein and zeaxanthin were the dominant carotenoid components. A rapid reduction in the transcription level of LCYε could result in a lower lutein production rate . CONCLUSION: Black sweet corn has a high nutritional value and is rich in vitamins, including zeaxanthin, γ-tocotrienols, and ascorbic acid. The best harvest time is between 20-25 days after pollination (DAPs) when kernels had a good taste as well as relatively high vitamin levels. © 2020 Society of Chemical Industry.
Asunto(s)
Semillas/crecimiento & desarrollo , Vitaminas/biosíntesis , Zea mays/metabolismo , Carotenoides/análisis , Carotenoides/metabolismo , Color , Luteína/análisis , Luteína/metabolismo , Semillas/química , Semillas/metabolismo , Tocotrienoles/análisis , Tocotrienoles/metabolismo , Vitaminas/análisis , Zea mays/química , Zea mays/crecimiento & desarrollo , Zeaxantinas/análisis , Zeaxantinas/metabolismoRESUMEN
For the controversy still existed about the oxidation stability of the high oleic oils compared with palm oil (PO), this study was aimed to explore the possible reason causing the controversies. Total polar compounds (TPC) was used to evaluate the oxidation stability of oils. Results showed there exist two kinds of lineal changes about the content of total polar compounds (TPC) in each oil, which were closely linked with the fatty acid composition and the tocochromanols content. The possible influence of the initial quality of oils also should be considered. The TPC of high oleic peanut oil (HOPO), high oleic sunflower oil (HOSO), high oleic rapeseed oil (HORO) and PO increased slowly at the initial period mainly owing to the antioxidation of tocochromanols, then sharply after 24, 48, 36 and 72 h respectively, when tocochromanols in each oil almost reduced below the detection limit. After that, the major factor would be fatty acids, particularly PUFA. It showed that the major tocochromanols in different oils (e.g. α, γ-tocotrienols in PO, α, γ-tocopherols in HORO and HOPO, and α-tocopherols in HOSO), could impose the main effects of inhibiting the TPC generation in the initial thermal treatment. The TPC in HORO significantly increased after 84 hours of heat process, which might be caused by the higher content of the polyunsaturated fatty acids (PUFA) (i.e. C18:2 and C18:3). However, the content of the saturated fatty acid (SFA) did not show statistically significant change during the thermal treatment.
Asunto(s)
Calidad de los Alimentos , Calor , Aceite de Palma/química , Aceite de Cacahuete/química , Aceite de Brassica napus/química , Aceite de Girasol/química , Antioxidantes/análisis , Ácidos Grasos/análisis , Ácidos Grasos Insaturados/análisis , Oxidación-Reducción , Factores de Tiempo , Tocoferoles/análisis , Tocotrienoles/análisisRESUMEN
Allium ampeloprasum L., commonly known as wild leek, is an edible vegetable that has been cultivated for centuries. However, no detailed studies have been undertaken to valorize A. ampeloprasum seed oil. This study aims to evaluate the physicochemical properties, chemical composition, and antioxidant activity of A. ampeloprasum seed oil. The seed oil content was found to be 18.20%. Gas chromatographymass spectrometry (GC-MS) showed that linoleic acid (71.65%) was the dominant acid, followed by oleic acid (14.11%) and palmitic acid (7.11%). A. ampeloprasum seed oil exhibited an oxidative stability of 5.22 h. Moreover, γ- and δ-tocotrienols were the major tocols present (79.56 and 52.08 mg/100 g oil, respectively). The total flavonoid content (16.64 µg CE /g oil) and total phenolic content (62.96 µg GAE /g oil) of the seed oil were also determined. The antioxidant capacity of the oil, as evaluated using the ABTS assay (136.30 µM TEAC/g oil), was found to be significant. These findings indicate that A. ampeloprasum seeds can be regarded as a new source of edible oil having health benefits and nutritional properties.
Asunto(s)
Antioxidantes/análisis , Semillas/química , Chalotes/química , Fenómenos Químicos , Flavonoides/análisis , Cromatografía de Gases y Espectrometría de Masas , Ácido Linoleico/análisis , Ácido Oléico/análisis , Ácido Palmítico/análisis , Fenoles/análisis , Tocotrienoles/análisisRESUMEN
The phytochemical contents, peroxyl radical scavenging capacities (PSCs) and cellular antioxidant activities (CAAs) of free and bound fractions of rice were reported. Black rice had the highest total phenolic content and total flavonoid content in free and bound fractions, followed by red rice, brown rice, and polished rice. Black rice contained much more free phenolic compounds than other rice samples, such as cyanidin-3-O-glucoside, protocatechuic acid, and vanillic acid. Tocopherols and tocotrienols contents were highest in red rice, then in black rice, brown rice, and polished rice. PSCs and CAAs of free and bound fractions were in the order: black rice > red rice > brown rice > polished rice, except that bound CAA of red rice was higher than that of black rice. The cellular uptake rate of free phenolics was highest in red rice, while cellular uptake rates of bound phenolics were highest in brown rice and polished rice.
Asunto(s)
Antioxidantes/análisis , Carcinoma Hepatocelular/tratamiento farmacológico , Flavonoides/análisis , Neoplasias Hepáticas/tratamiento farmacológico , Oryza/química , Fenoles/análisis , Fitoquímicos/análisis , Amidinas/efectos adversos , Antioxidantes/aislamiento & purificación , Antioxidantes/farmacología , Depuradores de Radicales Libres/metabolismo , Células Hep G2 , Humanos , Estrés Oxidativo/efectos de los fármacos , Peróxidos/metabolismo , Fitoquímicos/aislamiento & purificación , Fitoquímicos/farmacología , Especificidad de la Especie , Tocoferoles/análisis , Tocotrienoles/análisisRESUMEN
Maqui-berry (Aristotelia chilensis) is the emerging Chilean superfruit with high nutraceutical value. Until now, the research on this commodity was focused on the formulations enriched with polyphenols from the pulp. Herein, contents of tocols were compared in the seed oil of Maqui-berry obtained through three different extraction methods followed by determining their antioxidative and enzyme inhibitions in-vitro. Firstly, oilseed was extracted with n-hexane (Soxhlet method), chloroform/methanol/water (Bligh and Dyer method) and pressing (industrial). These samples were used to access their effects against DPPH, HORAC, ORAC, FRAP, Lipid-peroxidation (TBARS), α-amylase, α-glucosidase, and pancreatic lipase. All the isomers of tocopherol and tocotrienol were identified, and ß-sitosterol was the only sterol found in higher amounts than other vegetable oils. The Bligh and Dyer method could lead to the highest antioxidative capacity compared to Soxhlet and press methods likely because the latter have a higher amount of tocopherols. Further, seed oil from Maqui berry and their tocols (α, ß, γ, δ-tocopherols, tocotrienols, and ß-sitosterol) warrant clinical investigation for their antioxidative and antiobesity potential. Taken together, these findings provide relevant and suitable conditions for the industrial processing of Maqui-berry.
Asunto(s)
Fármacos Antiobesidad/farmacología , Antioxidantes/farmacología , Inhibidores Enzimáticos/farmacología , Sitoesteroles/farmacología , Tocoferoles/farmacología , Tocotrienoles/farmacología , Animales , Fármacos Antiobesidad/análisis , Antioxidantes/análisis , Inhibidores Enzimáticos/análisis , Lipasa/antagonistas & inhibidores , Magnoliopsida/química , Masculino , Ratas Wistar , Semillas/química , Sitoesteroles/análisis , Tocoferoles/análisis , Tocotrienoles/análisisRESUMEN
A method was established for the determination of eight vitamins E (α-, ß-, γ-, δ-tocopherol and α-, ß-, γ-, δ-tocotrienol) in vegetable oils using gas chromatography-mass spectrometry (GC-MS). The targets were extracted with methanol, and analyzed by GC-MS in the selected ion monitoring (SIM) mode after concentration to a constant volume, and quantified using the external standard method. Baseline separation were achieved for all the target compounds. The linearities of all the compounds were between 0.01 and 1 mg/L. The limits of detection (LODs) and limits of quantification (LOQs) were in the range of 0.03-0.25 mg/kg and 0.10-0.83 mg/kg, respectively. The average recoveries of all the targets in sesame oil samples were between 87.5% and 107.4% at three spiked levels (10, 50, and 250 mg/kg), and the RSDs were all less than 7.5%. The tocopherols and tocotrienols contents in sesame oil samples and in six lower-price vegetable oils (soybean, rapeseed, sunflower, peanut, corn and palm oils) were determined by the above mentioned method. The results showed that the vitamin E profiles of sesame oil were significantly different from those of the other six vegetable oils. Therefore, vitamin E can be used as a discriminating parameter for detecting the adulteration of sesame.
Asunto(s)
Análisis de los Alimentos , Contaminación de Alimentos/análisis , Aceites de Plantas/análisis , Tocotrienoles/análisis , Vitamina E/análisis , Cromatografía de Gases y Espectrometría de Masas , Aceite de Sésamo/análisisRESUMEN
A total of 101 local rice varieties, composted of 85 glutinous and 16 non-glutinous varieties grown in wet season 2016, were analyzed for tocopherols (TOC), tocotrienols (T3) and γ-oryzanol (Orz). Two popular varieties, RD6 and KDML105, were used as standard checks for glutinous and non-glutinous varieties, respectively. γ-TOC was found in all glutinous varieties (0.47-9.78 mg/kg), which were higher than RD6 (0.16 mg/kg). α-TOC was found in 40 varieties (1.02-6.29 mg/kg), only 6 varities were higher than RD6 (3.95 mg/kg). δ-T3 was found in 81 varieties (0.57-7.00 mg/kg), mostly varities were higher than RD6 (0.67 mg/kg). γ-T3 was found in all glutinous varieties (8.00-22.1 mg/kg), while RD6 contained 13.3 mg/kg. For α-T3, it was found in 39 glutinous varieties (1.52-9.94 mg/kg), mostly varities were higher than RD6 (2.62 mg/kg). Orz was found in all glutinous rice varieties (276-638 mg/kg), while RD6 contained 423 mg/kg. γ-TOC was found in all non-glutinous varieties (0.67-5.21 mg/kg), which were higher than KDML105. α-TOC was found in only 5 varieties (1.92-2.83 mg/kg), while KDML105 contained 2.23 mg/kg. δ-T3 was found in all non-glutinous varieties (1.64-8.87 mg/kg), which were higher than KDML105 (0.71 mg/kg). γ-T3 was also found in all non-glutinous varieties (8.80-17.58 mg/kg), while KDML105 contained 15.8 mg/kg. α-T3 was found in 9 non-glutinous varieties (4.56-8.93 mg/kg),while KDML105 contained only 0.86 mg/kg. Orz was also found in all those non-glutinous varieties (272-469 mg/kg), while KDML105 contained 469 mg/kg. These results indicate that γ-T3 was the highest vitamin E isomer present in all rice samples, while γ-TOC, α-TOC, δ-T3 and α-T3 were present in trace amounts. Orz was found in all local rice varieties.
Asunto(s)
Oryza/química , Fenilpropionatos/análisis , Tocoferoles/análisis , Tocotrienoles/análisis , TailandiaRESUMEN
Black rice is a variety of pigmented rice. It contains numerous nutritional and bioactive components, including essential amino acids, functional lipids, dietary fibre, vitamins, minerals, anthocyanins, phenolic compounds, γ-oryzanols, tocopherols, tocotrienols, phytosterols and phytic acid. There have been several studies of black rice due to its alleged beneficial health effects when consumed regularly. This review focuses on the historical aspects, chemical composition, and nutritional and functional properties of black rice. Furthermore, a discussion of the development of new foods and beverages with applications and processing technologies designed to improve their quality attributes. The nutritional value of black rice means that it has the potential to be used in the production of healthy foods and beverages, such as functional products and gluten-free cereals, thereby providing extra health benefits to consumers.