RESUMEN
Objective: To present a case of ocular toxoplasmosis. Materials and methods: A sixteen-year-old female patient presented to our clinic with complaints regarding decreased vision in her right eye (BCVA 0.5), starting five days before the exam. Her anamnestic data revealed a previous history of ocular toxoplasmosis in her left eye. OCT scans of the inner retina identified a huge cystic space, located posterior to the inner line, off the outer plexiform layer, with a small amount of hyperreflective foci. Other features of OCT included membranous-like structures on inner borders and elongation and splitting of the inner segment/outer segment junction. In later stages, beginning signs of retinitis and scaring could be observed. Results: The patient was treated with sulfamethoxazole/trimethoprim and prednisolone. After two weeks, total regression occurred and visual acuity and OCT remained stable for 6 months (BCVA 1.0). Discussion: Ocular toxoplasmosis can cause significant vision loss due to retinitis and scarring. Following treatment with sulfamethoxazole/trimethoprim and prednisolone, the patient's condition improved significantly and her visual acuity remained stable. Conclusion: On clinical examination and using OCT, rare morphological cystoid spaces (CS) can be identified as huge outer retina cysts (HORC), which are pathognomonic for posterior uveitis. Abbreviations: HORC = huge outer retinal cyst, OCT = optical coherence tomography, BCVA = best corrected visual acuity, CS = cyst space, OPL = outer plexiform layer, HRF = hyper reflective foci, RPE = retinal pigment epithelium, IS = inner segment, OS = outer segment, ERM = epiretinal membrane, PORT = punctate outer retinal toxoplasmosis, ELM = external limiting membrane.
Asunto(s)
Tomografía de Coherencia Óptica , Toxoplasmosis Ocular , Agudeza Visual , Humanos , Femenino , Toxoplasmosis Ocular/diagnóstico , Toxoplasmosis Ocular/tratamiento farmacológico , Toxoplasmosis Ocular/parasitología , Tomografía de Coherencia Óptica/métodos , Adolescente , Combinación Trimetoprim y Sulfametoxazol/uso terapéutico , Infecciones Parasitarias del Ojo/diagnóstico , Infecciones Parasitarias del Ojo/parasitología , Infecciones Parasitarias del Ojo/tratamiento farmacológico , Angiografía con Fluoresceína/métodos , Prednisolona/uso terapéutico , Retina/parasitología , Retina/patología , Glucocorticoides/uso terapéutico , Fondo de Ojo , Toxoplasma/aislamiento & purificaciónRESUMEN
Ocular toxoplasmosis (OT) is characterised by intraocular inflammation due to Toxoplasma gondii infection. Studies have found that interleukin 17 (IL-17) plays a central role in the pathology of OT. However, nucleotide variability in IL17 and interleukin 17 receptor (IL17R) genes has not been characterised in OT. As cytokine gene polymorphisms may influence the expression of these molecules, the aim of this study was to verify whether IL17A (rs2275913), IL17F (rs763780), IL17RA (rs4819554) and IL17RC (rs708567) polymorphisms are associated with OT in a Brazilian population. This study enrolled 214 patients seropositive for T. gondii (110 with OT and 104 without) and 107 controls. Polymorphisms were identified by PCR-restriction fragment length polymorphism analysis, validated by DNA sequencing with chi-square and multivariate analyses being used to assess possible associations between polymorphisms and OT. Logistic regression under the dominant model revealed a protection factor against OT of the C mutant allele of the IL17F (rs763780) polymorphism. The T/C-C/C genotypes were significantly more common in patients without OT compared to those with OT (p value = 0.0066) and controls (p value = 0.014). Findings from this study suggest that the IL17F polymorphism may have an influence in the immunopathology of OT in Brazilian individuals.
Asunto(s)
Interleucina-17 , Toxoplasmosis Ocular , Humanos , Toxoplasmosis Ocular/genética , Toxoplasmosis Ocular/inmunología , Toxoplasmosis Ocular/parasitología , Masculino , Femenino , Interleucina-17/genética , Adulto , Brasil , Persona de Mediana Edad , Adulto Joven , Polimorfismo de Nucleótido Simple , Predisposición Genética a la Enfermedad , Polimorfismo de Longitud del Fragmento de Restricción , Factores Protectores , Adolescente , Genotipo , Polimorfismo Genético , Reacción en Cadena de la Polimerasa , AncianoRESUMEN
PURPOSE: To study the role of Toxoplasma IgG avidity in evaluating the stage of systemic infection during manifestation as toxoplasma retinochoroiditis and its clinical implications in eastern India. METHODS: Retrospective chart review of Toxoplasma retinochoroiditis cases with Toxoplasma serology for IgG, IgM, and IgG avidity. RESULTS: Included in this study were 17 eyes of 17 patients who had active retinitis located in the macula (14), mid-periphery (2), or periphery (1). They were either primary lesions (12) or reactivations (5). All the cases had Toxoplasma IgG positive; one case had IgM positivity, while all the cases had high IgG avidity values. IgG avidity had a positive correlation with the duration of symptoms. CONCLUSION: We observed high IgG avidity values in active retinochoroiditis in both primary ocular Toxoplasmosis and reactivation subgroups. These results indicate a late ocular manifestation after initial systemic infection with a possible incubation period ranging from 5 weeks to 5 months.
Asunto(s)
Anticuerpos Antiprotozoarios , Coriorretinitis , Inmunoglobulina G , Toxoplasma , Toxoplasmosis Ocular , Humanos , Toxoplasmosis Ocular/inmunología , Toxoplasmosis Ocular/diagnóstico , Toxoplasmosis Ocular/parasitología , Estudios Retrospectivos , Inmunoglobulina G/sangre , Inmunoglobulina G/inmunología , Femenino , Masculino , Toxoplasma/inmunología , Coriorretinitis/parasitología , Coriorretinitis/inmunología , Coriorretinitis/diagnóstico , Adulto , Anticuerpos Antiprotozoarios/sangre , Anticuerpos Antiprotozoarios/inmunología , Adulto Joven , Adolescente , Niño , Infecciones Parasitarias del Ojo/parasitología , Infecciones Parasitarias del Ojo/diagnóstico , Infecciones Parasitarias del Ojo/inmunología , Inmunoglobulina M/sangre , Inmunoglobulina M/inmunología , Persona de Mediana Edad , Afinidad de Anticuerpos , Estudios de SeguimientoRESUMEN
Granulomatous anterior uveitis with single or numerous gelatinous nodules was found in children living in rural Egypt. All ocular diseases were originally thought to be water-born and related to digenic flukes. The current study sought to learn more about the causes of anterior granulomatous uveitis in Egyptian youngsters who used to swim in rural water canals. 50 children with eye lesions that had not responded to medical treatment were recruited. Four samples were surgically extracted and examined using real-time PCR, transmission electron microscopy (TEM), and shotgun metagenomic sequencing (SMS). Toxoplasma gondii was detected free within the syncytium's distal section, while the proximal part exhibited active synthesis of a presumably extra-polymeric material, possibly released by the microbial population. Toxoplasma gondii was found in 30 samples. Serologically, distinct anti-Toxoplasma antibodies were not found in 91.6% of patients. SMS showed that the T. gondii ME 49 strain had the greatest percentage (29-25%) in all samples within an Acinetobacter-containing microbial community. These findings suggested that these bacteria entered the body via the exterior route rather than the circulatory route. The lack of genetic evidence for subsequent parasite stages invalidates the prior findings about the assumed trematode stage.
Asunto(s)
Toxoplasma , Toxoplasmosis Ocular , Uveítis , Niño , Humanos , Toxoplasmosis Ocular/diagnóstico , Toxoplasmosis Ocular/epidemiología , Toxoplasmosis Ocular/parasitología , Egipto/epidemiología , Uveítis/parasitología , Ojo , Toxoplasma/genética , Anticuerpos Antiprotozoarios , Agua/análisisRESUMEN
BACKGROUND: Toxoplasmosis is a zoonotic illness caused by Toxoplasma gondii. Ocular infection frequently manifests as acute necrotizing retinal chorioretinitis. In this paper, we describe a case of retinal chorioretinitis caused by Toxoplasma gondii infection, as well as the most recent diagnostic and treatment techniques. METHODS: Serum and vitreous fluid were collected and analyzed, and PCR for Toxoplasma gondii DNA, ELISA for Toxoplasma gondii IgG and Goldmann-Witmer coefficient, fundus fluorescein angiography (FFA), indocyanine green angiography (ICGA), and fundus autofluorescence were done (FAF). RESULTS: Toxoplasma gondii DNA (-), serum and vitreous IgG from Toxoplasma gondii (+) cells, and the Goldmann-Witmer coefficient of Toxoplasma gondii were all considerably enhanced, indicating Toxoplasma gondii infection. Antiparasitic infection in combination with an anti-inflammatory glucocorticoid were given, laser treatment of the fundus was provided, and the patient's condition has been stable with no indication of recurrence to date following conclusion of therapy. CONCLUSIONS: Toxoplasma gondii can infect the whole retina, causing variable degrees of visual impairment; thus, rapid diagnosis and tailored therapy are necessary to enhance prognosis and reduce disease recurrence.
Asunto(s)
Coriorretinitis , Toxoplasma , Toxoplasmosis Ocular , Humanos , Toxoplasmosis Ocular/diagnóstico , Toxoplasmosis Ocular/parasitología , Coriorretinitis/diagnóstico , Coriorretinitis/parasitología , Toxoplasma/genética , Reacción en Cadena de la Polimerasa/métodos , Anticuerpos Antiprotozoarios , Inmunoglobulina GRESUMEN
PURPOSE: To present a narrative review about ocular toxoplasmosis epidemiology, disease burden and prevalent African parasitic strains. METHODS: An initial search for MeSH terms was conducted with a posterior advanced search in two electronic databases. Full text reading was performed. RESULTS: Animal African studies have identified Toxoplasma gondii type II, type III, Africa 1, and Africa 3 strains. Seroprevalence varies from 6.4% to 74.5%. Nevertheless, there is a scarcity of epidemiology and serotyping information about ocular toxoplasmosis. African studies have demonstrated that uveitis patients present high frequencies of ocular toxoplasmosis. There is a lack of studies describing specific clinical characteristics, which can be related, to environmental and socioeconomic factors, parasite serotype and genotype, and genetic susceptibility of the host. CONCLUSION: As Toxoplasma gondii has more virulent strains in the Southern hemisphere, it is relevant to determine African strain types and the correlation between the infecting strains and the clinical manifestations.
Asunto(s)
Toxoplasma , Toxoplasmosis Ocular , África/epidemiología , Animales , Genotipo , Humanos , Estudios Seroepidemiológicos , Toxoplasma/genética , Toxoplasmosis Ocular/diagnóstico , Toxoplasmosis Ocular/epidemiología , Toxoplasmosis Ocular/parasitologíaRESUMEN
Toxoplasma gondii infection can trigger autoreactivity by different mechanisms. In the case of ocular toxoplasmosis, disruption of the blood-retinal barrier may cause exposure of confined retinal antigens such as recoverin. Besides, cross-reactivity can be induced by molecular mimicry of parasite antigens like HSP70, which shares 76% identity with the human ortholog. Autoreactivity can be a determining factor of clinical manifestations in the eye and in the central nervous system. We performed a prospective observational study to determine the presence of autoantibodies against recoverin and HSP70 by indirect ELISA in the serum of 65 patients with ocular, neuro-ophthalmic and congenital cerebral toxoplasmosis. We found systemic autoantibodies against recoverin and HSP70 in 33.8% and 15.6% of individuals, respectively. The presence of autoantibodies in cases of OT may be related to the severity of clinical manifestations, while in cases with CNS involvement they may have a protective role. Unexpectedly, anti-recoverin antibodies were found in patients with cerebral involvement, without ocular toxoplasmosis; therefore, we analyzed and proved cross-reactivity between recoverin and a brain antigen, hippocalcin, so the immunological phenomenon occurring in one immune-privileged organ (e.g. the central nervous system) could affect the environment of another (egg. the eye).
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Autoanticuerpos/inmunología , Autoantígenos/inmunología , Interacciones Huésped-Parásitos/inmunología , Toxoplasmosis Cerebral/inmunología , Toxoplasmosis Congénita/inmunología , Toxoplasmosis Ocular/inmunología , Adolescente , Adulto , Secuencia de Aminoácidos , Antígenos de Protozoos/inmunología , Niño , Preescolar , Reacciones Cruzadas/inmunología , Femenino , Proteínas HSP70 de Choque Térmico/química , Proteínas HSP70 de Choque Térmico/inmunología , Hipocalcina/química , Hipocalcina/inmunología , Humanos , Lactante , Recién Nacido , Masculino , Persona de Mediana Edad , Recoverina/química , Recoverina/inmunología , Toxoplasma/inmunología , Toxoplasmosis Cerebral/diagnóstico , Toxoplasmosis Cerebral/parasitología , Toxoplasmosis Congénita/diagnóstico , Toxoplasmosis Congénita/parasitología , Toxoplasmosis Ocular/diagnóstico , Toxoplasmosis Ocular/parasitología , Adulto JovenRESUMEN
PURPOSE: To determine classification criteria for toxoplasmic retinitis. DESIGN: Machine learning of cases with toxoplasmic retinitis and 4 other infectious posterior uveitides / panuveitides. METHODS: Cases of infectious posterior uveitides / panuveitides were collected in an informatics-designed preliminary database, and a final database was constructed of cases achieving supermajority agreement on diagnosis, using formal consensus techniques. Cases were split into a training set and a validation set. Machine learning using multinomial logistic regression was used on the training set to determine a parsimonious set of criteria that minimized the misclassification rate among the infectious posterior uveitides / panuveitides. The resulting criteria were evaluated on the validation set. RESULTS: Eight hundred three cases of infectious posterior uveitides / panuveitides, including 174 cases of toxoplasmic retinitis, were evaluated by machine learning. Key criteria for toxoplasmic retinitis included focal or paucifocal necrotizing retinitis and either positive polymerase chain reaction assay for Toxoplasma gondii from an intraocular specimen or the characteristic clinical picture of a round or oval retinitis lesion proximal to a hyperpigmented and/or atrophic chorioretinal scar. Overall accuracy for infectious posterior uveitides / panuveitides was 92.1% in the training set and 93.3% (95% confidence interval 88.2, 96.3) in the validation set. The misclassification rates for toxoplasmic retinitis were 8.2% in the training set and 10% in the validation set. CONCLUSIONS: The criteria for toxoplasmic retinitis had a low misclassification rate and seemed to perform sufficiently well for use in clinical and translational research.
Asunto(s)
Humor Acuoso/parasitología , Infecciones Parasitarias del Ojo/clasificación , Aprendizaje Automático , Retinitis/clasificación , Toxoplasma/aislamiento & purificación , Toxoplasmosis Ocular/clasificación , Adulto , Animales , Anticuerpos Antiprotozoarios/análisis , ADN Protozoario/análisis , Infecciones Parasitarias del Ojo/diagnóstico , Infecciones Parasitarias del Ojo/parasitología , Femenino , Humanos , Masculino , Retinitis/diagnóstico , Retinitis/parasitología , Toxoplasma/genética , Toxoplasma/inmunología , Toxoplasmosis Ocular/diagnóstico , Toxoplasmosis Ocular/parasitología , Adulto JovenRESUMEN
Purpose: To describe a case of presumed ocular toxoplasmosis.Method: A retrospective chart review.Result: This case report describes a 35-year-old male who presented hypopyon anterior uveitis in his left eye. Fundus examination revealed severe vitritis which precluded the view of retina. His serum anti-toxoplasma immunoglobulin (Ig)G was significantly raised, with a normal anti-toxoplasma IgM level and PCR from nested primers targeting B1 gene of Toxoplasma gondii was positive. He was started on empirical anti-toxoplasma therapy. Undiluted vitreous specimen collected during lensectomy and diagnostic vitrectomy in left eye was also positive for nested primers targeting B1 gene of T. gondii.Conclusion: Ocular toxoplasmosis without retinochoroiditis, though extremely rare, can occur.
Asunto(s)
Manejo de la Enfermedad , Endoftalmitis/diagnóstico , Infecciones Parasitarias del Ojo/diagnóstico , Toxoplasmosis Ocular/diagnóstico , Adulto , Antibacterianos/uso terapéutico , ADN Protozoario/análisis , Endoftalmitis/parasitología , Endoftalmitis/terapia , Infecciones Parasitarias del Ojo/parasitología , Infecciones Parasitarias del Ojo/terapia , Humanos , Masculino , Estudios Retrospectivos , Toxoplasma/genética , Toxoplasmosis Ocular/parasitología , Toxoplasmosis Ocular/terapia , Vitrectomía , Cuerpo Vítreo/parasitología , Cuerpo Vítreo/patología , Cuerpo Vítreo/cirugíaRESUMEN
PURPOSE: There are currently limited data addressing the surgical outcomes of pars plana vitrectomy (PPV) in toxoplasmosis-related macular hole (tMH). We aim to report and discuss safety and efficacy of PPV for tMH. METHODS: Surgical case series (n = 11), with minimum postoperative follow-up time of 6 months. Consecutive patients who underwent PPV for tMH from 2013 to 2016 were included. Indications for surgery were: visual acuity ≥ 0.6 logarithm of the minimum angle of resolution (Snellen 20/80 or less), no intraocular inflammation for more than 6 months, extrafoveal toxoplasmosis scar, elevated tMH borders on optical coherence tomography, and patient agreement with surgery. Surgery was performed-PPV with epiretinal (if present) and internal limiting membrane peeling. Safety and efficacy of PPV for tMH were addressed by evaluating: 1) surgery-related complications and 2) visual acuity improvement. RESULTS: A total of 11 patients (6 male), with a mean age of 33.2 ± 11.0 years were studied. Mean preoperative best-corrected visual acuity significantly improved from 1.10 ± 0.24 (Snellen 20/252) to 0.43 ± 0.18 logarithm of the minimum angle of resolution (Snellen 20/54) at last follow-up visit (P < 0.01). The rate of visual acuity improvement (i.e., a gain of at least three lines) and tMH closure was 100% for both. The only reported surgery-related complication was cataract in one case. CONCLUSION: Our results suggest that PPV is a safe and effective option in tMH cases. A controlled, longitudinal study would contribute to confirm these findings.
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Perforaciones de la Retina/cirugía , Toxoplasmosis Ocular/cirugía , Vitrectomía , Adolescente , Adulto , Membrana Basal/cirugía , Membrana Epirretinal/cirugía , Femenino , Humanos , Masculino , Perforaciones de la Retina/parasitología , Perforaciones de la Retina/fisiopatología , Estudios Retrospectivos , Tomografía de Coherencia Óptica , Toxoplasmosis Ocular/parasitología , Toxoplasmosis Ocular/fisiopatología , Agudeza Visual/fisiología , Adulto JovenRESUMEN
DUSN is an infectious ocular disease that can lead to severe visual impairment and blindness. It usually occurs in young healthy individuals and depending on the stage of the disease, clinical presentation may range from mild vitritis and multifocal gray-white lesions in outer retina to optic atrophy.Parasites of different sizes and species have been proposed as the etiological agent of this disease. Thus, it is hypothesized that different infectious worms may be considered as the likely cause of a both autoimmune and toxic form of nematode retinopathy.Most patients present with already severe visual impairment and in the later stages of the disease, where the likelihood of improvement is low, despite therapy. In cases of early diagnosis, prompt treatment, whether with oral antihelmintic or direct photocoagulation of the worm, patients may show considerable visual improvement and have a more favorable prognosis.
Asunto(s)
Infecciones Parasitarias del Ojo/complicaciones , Granuloma/complicaciones , Mácula Lútea/patología , Retinitis/etiología , Toxoplasma/aislamiento & purificación , Toxoplasmosis Ocular/complicaciones , Agudeza Visual , Adolescente , Diagnóstico Diferencial , Infecciones Parasitarias del Ojo/diagnóstico , Infecciones Parasitarias del Ojo/parasitología , Angiografía con Fluoresceína/métodos , Fondo de Ojo , Granuloma/diagnóstico , Granuloma/parasitología , Humanos , Mácula Lútea/parasitología , Masculino , Retinitis/diagnóstico , Retinitis/parasitología , Tomografía de Coherencia Óptica , Toxoplasmosis Ocular/diagnóstico , Toxoplasmosis Ocular/parasitologíaRESUMEN
Background: Endogenous fungal endophthalmitis is a sight-threatening condition with potentially devastating outcome. Hematogenous spread of the infective seedings is the route of infection. Infected individuals have usually a compromised immune status. The clinical picture of mycotic endogenous endophthalmitis is commonly seen as chorioretinitis. Candida is the most common fungus. Cladosporium causing endogenous endophthalmitis is a rare occurrence, with only a few cases published.Methods: The report includes study and management of a diabetic patient with endogenous cladosporium endophthalmitis mimicking toxoplasma retinochoroiditis.Results: Diagnosis was confirmed as Cladosporium Cladosporioides in vitreous and aqueous aspirate by polymerase chain reaction-based DNA sequencing. Patient was successfully managed with intravitreal and systemic voriconazole.Conclusion: Cladosporium can cause endogenous endophthalmitis and mimic toxoplasma retinochoroiditis. Vitreous biopsy can help in diagnosis in the absence of positive blood culture. Intravitreal voriconazole along with systemic voriconazole shows a good response.
Asunto(s)
Coriorretinitis/diagnóstico , Cladosporium/aislamiento & purificación , Endoftalmitis/diagnóstico , Infecciones Fúngicas del Ojo/diagnóstico , Micosis/diagnóstico , Toxoplasmosis Ocular/diagnóstico , Adulto , Antifúngicos/uso terapéutico , Humor Acuoso/microbiología , Coriorretinitis/parasitología , Cladosporium/genética , ADN de Hongos/genética , Endoftalmitis/tratamiento farmacológico , Endoftalmitis/microbiología , Infecciones Fúngicas del Ojo/tratamiento farmacológico , Infecciones Fúngicas del Ojo/microbiología , Humanos , Masculino , Micosis/tratamiento farmacológico , Micosis/microbiología , Reacción en Cadena de la Polimerasa , Toxoplasmosis Ocular/parasitología , Cuerpo Vítreo/microbiología , Voriconazol/uso terapéuticoRESUMEN
BACKGROUND: Dosing recommendations for the treatment of pregnancy-acquired toxoplasmosis are empirical and widely based on experimental data. There are no pharmacological data on pregnant women with acute Toxoplasma gondii infection under treatment with pyrimethamine (PY) and sulfadiazine (SA) and our study intends to tighten this gap. METHODS: In this retrospective case-control study, we included 89 pregnant women with primary Toxoplasma infection (PT) treated with PY (50 mg first dose, then 25 mg/day), SA (50 mg/kg of body weight/day), and folinic acid (10-15 mg per week). These were compared to a group of 17 women with acute ocular toxoplasmosis (OT) treated with an initial PY dose of 75 mg, thereafter 25 mg twice a day but on the same SA and folinic acid regimen. The exact interval between drug intake and blood sampling and co-medication had not been recorded. Plasma levels of PY and SA were determined 14 ± 4 days after treatment initiation using liquid chromatography-mass spectrometry and compared using the Mann-Whitney U test at a p < 0.05 level. RESULTS: In 23 PT patients (26%), SA levels were below 20 mg/l. Fifteen of these 23 patients (17% of all patients) in parallel presented with PY levels below 700 µg/l. Both drug concentrations differed remarkably between individuals and groups (PY: PT median 810 µg/l, 95% CI for the median [745; 917] vs. OT 1230 µg/l [780; 1890], p = 0.006; SA: PT 46.2 mg/l [39.9; 54.4] vs. OT 70.4 mg/l [52.4; 89], p = 0.015) despite an identical SA dosing scheme. CONCLUSIONS: SA plasma concentrations were found in the median 34% lower in pregnant women with PT compared to OT patients and fell below a lower reference value of 50 mg/l in a substantial portion of PT patients. The interindividual variability of plasma concentrations in combination with systematically lower drug levels and possibly a lower compliance in pregnant women may thus account for a still not yet supportable transmission risk. Systematic drug-level testing in PT under PY/SA treatment deserves to be considered.
Asunto(s)
Complicaciones Parasitarias del Embarazo/tratamiento farmacológico , Pirimetamina/uso terapéutico , Sulfadiazina/uso terapéutico , Toxoplasma/efectos de los fármacos , Toxoplasmosis Ocular/tratamiento farmacológico , Toxoplasmosis/tratamiento farmacológico , Adolescente , Adulto , Antiprotozoarios/sangre , Antiprotozoarios/uso terapéutico , Estudios de Casos y Controles , Quimioterapia Combinada , Femenino , Humanos , Embarazo , Complicaciones Parasitarias del Embarazo/parasitología , Pirimetamina/sangre , Estudios Retrospectivos , Sulfadiazina/sangre , Toxoplasma/fisiología , Toxoplasmosis/parasitología , Toxoplasmosis Ocular/parasitología , Resultado del Tratamiento , Adulto JovenRESUMEN
PURPOSE: To highlight the advantages of optical coherence tomography angiography (OCTA) in delineating the morphological features of the retinal and choroidal vascular network during acute, relapsing, and quiescent stages of macular toxoplasma retinochoroiditis. METHODS: This prospective study included patients presenting with both active and quiescent ocular toxoplasmoses. OCTA was obtained to diagnose and follow the subsequent vascular network changes at diagnosis and six months after acute presentation. RESULTS: Twenty-three eyes of 23 patients were included. In active lesions, OCTA showed extensive, well-delineated areas of intense hyposignal and perifoveal capillary arcade disruption in the parafoveal superficial capillary plexus (pSCP) and less extensive hyposignal in the parafoveal deep capillary plexus (pDCP). Signals of decreased deep capillary density and disorganization were also seen in the choroid. In nonactive lesions, OCTA demonstrated a homogenous and equally attenuated grayish hyposignal of the pSCP and pDCP and a partial restoration of the nonperfused choroidal areas. CONCLUSION: OCTA is a useful technique for vascular network analysis in toxoplasma retinochoroiditis. It allows the visualization of the different network changes and behaviors during the different stages of the infection.
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Coroides/patología , Microvasos/patología , Retina/patología , Vasos Retinianos/patología , Toxoplasma/patogenicidad , Toxoplasmosis Ocular/patología , Adulto , Capilares/parasitología , Capilares/patología , Coroides/parasitología , Femenino , Angiografía con Fluoresceína/métodos , Humanos , Masculino , Microvasos/parasitología , Estudios Prospectivos , Retina/parasitología , Vasos Retinianos/parasitología , Tomografía de Coherencia Óptica/métodos , Toxoplasmosis Ocular/parasitología , Agudeza Visual/fisiologíaRESUMEN
Peripheral blood mononuclear cells (PBMC) from patients with ocular toxoplasmosis were challenged with total antigens from Toxoplasma gondii lysate (TATL) in a cytokine release assay (CRA), run during the inactive period of the disease. Increased interferon gamma (IFN-γ) levels were detected after PBMC stimulation with either ME49 reference strain (P = 0.0015) or local TgCkAr-11-9 isolate (P = 0.0012), as compared with those recorded under basal conditions. TATL from TgCkAr11-9 isolate induced a higher release of IFN-γ than ME49 strain in CRA from all tested patients (P = 0.02). The median value of IFN-γ release on TgCkAr-11-9 stimulation (26.03 pg/mL) allowed the classification of patients into high- or low-/non-IFN-γ releasers. Clinical correlations were established with both groups. The results obtained in this study suggest the need to include local strains when performing CRA with TATL.
Asunto(s)
Interferón gamma/sangre , Interleucina-10/sangre , Toxoplasma/inmunología , Toxoplasmosis Ocular/inmunología , Adulto , Anciano , Femenino , Humanos , Leucocitos Mononucleares/inmunología , Masculino , Persona de Mediana Edad , Toxoplasmosis Ocular/parasitología , Adulto JovenRESUMEN
A 9-year-old otherwise healthy boy was examined due to a 4-day history of visual decline in his right eye. Ophthalmological examination revealed an area of active retinochoroiditis in the right macula. Indocyanine green angiography (ICGA) demonstrated multiple hypocyanescent dots surrounding the active lesion extending 360 degrees towards the equator. Optical coherence tomography angiography (OCTA) exhibited dark dots on the choriocapillaris slab over areas corresponding to the hypocyanescent dots detected with ICGA. Full systemic examination and laboratory investigations were carried out. Toxoplasma gondii serology was positive. The diagnosis of toxoplasmic chorioretinitis with white dot-like choroidal involvement was made. Trimethoprim/sulfamethoxazole, azithromycin, and oral prednisolone were administered orally. On repeated ICGA 2 weeks later, the scattered hypocyanescent dots were significantly fewer in number. A month later, right visual acuity was improved, the macular chorioretinitis focus had become inactive, an epiretinal membrane had formed, and the dark dots on the choriocapillaris slab of OCTA were markedly diminished. ICGA may be helpful to observe possible, subtle choroidal involvement in patients with toxoplasmic chorioretinitis.
Asunto(s)
Coriorretinitis/diagnóstico , Coroides/patología , Infecciones Parasitarias del Ojo/diagnóstico , Angiografía con Fluoresceína/métodos , Verde de Indocianina/farmacología , Tomografía de Coherencia Óptica/métodos , Toxoplasmosis Ocular/diagnóstico , Enfermedad Aguda , Animales , Niño , Coriorretinitis/parasitología , Coroides/parasitología , Colorantes/farmacología , Infecciones Parasitarias del Ojo/parasitología , Fondo de Ojo , Humanos , Masculino , Toxoplasma/aislamiento & purificación , Toxoplasmosis Ocular/parasitología , Agudeza VisualAsunto(s)
Infecciones Parasitarias del Ojo/complicaciones , Angiografía con Fluoresceína/métodos , Oclusión de la Arteria Retiniana/etiología , Arteria Retiniana/diagnóstico por imagen , Retinitis/complicaciones , Toxoplasma/aislamiento & purificación , Toxoplasmosis Ocular/complicaciones , Adulto , Infecciones Parasitarias del Ojo/diagnóstico , Infecciones Parasitarias del Ojo/parasitología , Fondo de Ojo , Humanos , Masculino , Oclusión de la Arteria Retiniana/diagnóstico , Retinitis/diagnóstico , Toxoplasmosis Ocular/diagnóstico , Toxoplasmosis Ocular/parasitologíaRESUMEN
OBJECTIVE: This study aimed to perform genotyping of Toxoplasma gondii strain or variant causing atypical toxoplasmic uveitis in Indonesian patients. METHODS: Ocular fluid samples originating from 46 uveitis patients with non-specific ocular manifestations were analysed for Toxoplasma infection by PCR of the B1 locus. The clonal type was determined by amplification, sequencing and phylogenetic analysis of SAG2 and GRA6 loci in B1-positive samples. Clinical data were obtained from the medical records. RESULTS: Pan uveitis was the most frequent manifestation (65.2%) and mostly unilateral (76.1%). PCR of the B1 locus identified eight positive subjects (12.5%); six had panuveitis and two of these had diabetes mellitus. Phylogenetic analysis with maximum likelihood of the SAG2 locus in the B1-positive samples resulted in Toxoplasma gondii SAG2 type III allele. No positive result was obtained from the PCR of GRA6 locus. CONCLUSION: Toxoplasma gondii SAG2-type III allele was identified in an atypical presentation of toxoplasmic uveitis in Indonesia.
Asunto(s)
Toxoplasma/aislamiento & purificación , Toxoplasmosis Ocular/parasitología , Adulto , Anciano , Alelos , Animales , ADN Protozoario/genética , Femenino , Genotipo , Humanos , Indonesia/epidemiología , Masculino , Persona de Mediana Edad , Filogenia , Reacción en Cadena de la Polimerasa , Polimorfismo de Longitud del Fragmento de Restricción , Toxoplasma/clasificación , Toxoplasma/genética , Toxoplasmosis Ocular/epidemiología , Adulto JovenRESUMEN
PURPOSE: To compare the effects of 1 year of treatment with trimethoprim-sulfamethoxazole (TMP-SMZ) vs placebo in reducing the risk of recurrence of toxoplasmic retinochoroiditis during a 6-year follow-up period. DESIGN: Randomized, double-masked clinical trial. METHODS: This cohort included 141 subjects recruited in Campinas, Brazil. The inclusion criterion was unilateral active recurrent toxoplasmic retinochoroiditis. All subjects were treated with 1 dose of TMP-SMZ (160 mg/800 mg) twice daily for 45 days, and all lesions healed after this treatment. After this initial treatment, subjects were randomly assigned to group 1 (1 TMP-SMZ dose every other day for 311 days) or group 2 (1 identical placebo tablet containing starch with no active ingredients every other day for 311 days). Between the second and sixth years of follow-up appointments, none of the subjects received treatment unless a new recurrence episode had occurred. The primary outcomes were recurrent toxoplasmic retinochoroiditis within the first year of follow-up and recurrent toxoplasmic retinochoroiditis in the 6 years of follow-up. RESULTS: The cumulative probability of recurrence 1, 2, 3, 4, 5, and 6 years after the initial infection was, respectively, 13.0% (9/69), 17.4% (12/69), 20.3% (14/69), 23.2% (16/69), 26.1% (18/69), and 27.5% (19/69) in the placebo group and 0%, 0%, 0%, 0%, 0%, and 1.4% (1/72) in the TMP-SMZ group (P < .001; log-rank test). There were 3 cases (3/69; 4.3%) of multiple recurrences in the same individual in the placebo group. No treatment-limiting toxicity or side effects were observed in either group. New recurrences were more frequent among female subjects. CONCLUSIONS: TMP-SMZ may be used safely for prophylaxis of recurrent toxoplasmic retinochoroiditis and may provide long-term benefits.
Asunto(s)
Antibacterianos/uso terapéutico , Coriorretinitis/prevención & control , Infecciones Parasitarias del Ojo/prevención & control , Toxoplasmosis Ocular/prevención & control , Combinación Trimetoprim y Sulfametoxazol/uso terapéutico , Adulto , Coriorretinitis/diagnóstico , Coriorretinitis/parasitología , Método Doble Ciego , Infecciones Parasitarias del Ojo/diagnóstico , Infecciones Parasitarias del Ojo/parasitología , Femenino , Estudios de Seguimiento , Humanos , Masculino , Persona de Mediana Edad , Estudios Prospectivos , Recurrencia , Prevención Secundaria , Toxoplasmosis Ocular/diagnóstico , Toxoplasmosis Ocular/parasitología , Agudeza Visual/fisiología , Adulto JovenRESUMEN
BACKGROUND: Ocular toxoplasmosis is a common cause of ocular inflammation worldwide. The aim of this study is to characterize the clinical outcomes and lesion evolution of patients with ocular toxoplasmosis and to compare the primary and reactivation subgroups. METHODS: A retrospective population-based cohort study at one uveitis-specialized tertiary referral center. Patients presenting with active ocular toxoplasmosis between the years 2007-2016 were included. Primary ocular toxoplasmosis and reactivations were compared. RESULTS: Included were 22 patients, 64% female with a mean age of 29 ± 18 years, 59% (n = 13) were primary, 9% (n = 2) congenital and 32% (n = 7) reactivations. Visual acuity improved from 0.38 ± 0.44 to 0.20 ± 0.27 LogMAR (P = 0.026) after a mean of 37 ± 33 months. Initial lesion size was 2.38 ± 1.1 optic disc areas, reducing to 1.56 ± 1.24 following 2 months (34% reduction, P = 0.028) and to 1.17 ± 0.87 disc areas following one year (51% reduction, P = 0.012). Patients with macula-threatening lesions had worse visual acuity (0.50 ± 0.46 vs. 0.05 ± 0.07 LogMAR, P = 0.047). Primary and reactivation subgroups had similar presentations, visual outcomes and recurrence rates (all P > 0.05). CONCLUSIONS: In this population, primary ocular toxoplasmosis was the most common presentation. Lesion size reduced during the initial months with limited change thereafter and a third of cases recurred. Macula-threatening lesions were associated with worse visual acuity, and no significant differences were seen between the primary and reactivation subgroups.