RESUMEN
MicroRNA-885 (miR-885) has been shown to act as vital regulator of tumorigenesis and its tumor-suppressive role has been investigated in several human cancers. However, the role of miR-885 in regulation of epithelial mesenchymal transition of liver cancer cells yet unknown. This study was undertaken to investigate the tumor-suppressive role of miR-885 and investigate its effects on epithelial mesenchymal transition of human liver cancer cells. The results revealed that miR-885 to be significantly (P < 0.05) repressed in liver cancer and tissues and cell lines. Overexpression of miR-885 resulted in significant (P < 0.05) decline in the proliferation of liver cancer cells. Additionally, migration and invasion of the liver cancer cells was also suppressed upon miR-182 overexpression which was associated with alteration of the proteins associated with epithelial mesenchymal transition. TMOD1 was identified as the target of miR-885 and the regulatory role of miR-885 was elucidated to be exerted via post-transcriptional silencing of TMOD1. The silencing of TMOD1 by miR-885 inhibited the expression of mesenchymal markers but enhanced the expression levels of epithelial markers. The results of present study revealed miR-885 proved the tumor-suppressive role of miR-885 in liver cancer and points towards its therapeutic implications in liver cancer management.
Asunto(s)
Carcinoma Hepatocelular/patología , Transición Epitelial-Mesenquimal/fisiología , Neoplasias Hepáticas/patología , MicroARNs/fisiología , Tropomodulina/fisiología , Adulto , Femenino , Humanos , Masculino , Persona de Mediana EdadRESUMEN
Neurons of the CNS elaborate highly branched dendritic arbors that host numerous dendritic spines, which serve as the postsynaptic platform for most excitatory synapses. The actin cytoskeleton plays an important role in dendrite development and spine formation, but the underlying mechanisms remain incompletely understood. Tropomodulins (Tmods) are a family of actin-binding proteins that cap the slow-growing (pointed) end of actin filaments, thereby regulating the stability, length, and architecture of complex actin networks in diverse cell types. Three members of the Tmod family, Tmod1, Tmod2, and Tmod3 are expressed in the vertebrate CNS, but their function in neuronal development is largely unknown. In this study, we present evidence that Tmod1 and Tmod2 exhibit distinct roles in regulating spine development and dendritic arborization, respectively. Using rat hippocampal tissues from both sexes, we find that Tmod1 and Tmod2 are expressed with distinct developmental profiles: Tmod2 is expressed early during hippocampal development, whereas Tmod1 expression coincides with synaptogenesis. We then show that knockdown of Tmod2, but not Tmod1, severely impairs dendritic branching. Both Tmod1 and Tmod2 are localized to a distinct subspine region where they regulate local F-actin stability. However, the knockdown of Tmod1, but not Tmod2, disrupts spine morphogenesis and impairs synapse formation. Collectively, these findings demonstrate that regulation of the actin cytoskeleton by different members of the Tmod family plays an important role in distinct aspects of dendrite and spine development.SIGNIFICANCE STATEMENT The Tropomodulin family of molecules is best known for controlling the length and stability of actin myofilaments in skeletal muscles. While several Tropomodulin members are expressed in the brain, fundamental knowledge about their role in neuronal function is limited. In this study, we show the unique expression profile and subcellular distribution of Tmod1 and Tmod2 in hippocampal neurons. While both Tmod1 and Tmod2 regulate F-actin stability, we find that they exhibit isoform-specific roles in dendrite development and synapse formation: Tmod2 regulates dendritic arborization, whereas Tmod1 is required for spine development and synapse formation. These findings provide novel insight into the actin regulatory mechanisms underlying neuronal development, thereby shedding light on potential pathways disrupted in a number of neurological disorders.
Asunto(s)
Dendritas/fisiología , Hipocampo/crecimiento & desarrollo , Sinapsis/fisiología , Tropomodulina/fisiología , Animales , Células Cultivadas , Dendritas/química , Femenino , Hipocampo/química , Hipocampo/citología , Masculino , Neuronas/química , Neuronas/fisiología , Embarazo , Isoformas de Proteínas/química , Isoformas de Proteínas/fisiología , Ratas , Ratas Sprague-Dawley , Sinapsis/químicaRESUMEN
The actin cytoskeleton is important for platelet biogenesis. Tropomodulin-3 (Tmod3), the only Tmod isoform detected in platelets and megakaryocytes (MKs), caps actin filament (F-actin) pointed ends and binds tropomyosins (TMs), regulating actin polymerization and stability. To determine the function of Tmod3 in platelet biogenesis, we studied Tmod3(-/-) embryos, which are embryonic lethal by E18.5. Tmod3(-/-) embryos often show hemorrhaging at E14.5 with fewer and larger platelets, indicating impaired platelet biogenesis. MK numbers are moderately increased in Tmod3(-/-) fetal livers, with only a slight increase in the 8N population, suggesting that MK differentiation is not significantly affected. However, Tmod3(-/-) MKs fail to develop a normal demarcation membrane system (DMS), and cytoplasmic organelle distribution is abnormal. Moreover, cultured Tmod3(-/-) MKs exhibit impaired proplatelet formation with a wide range of proplatelet bud sizes, including abnormally large proplatelet buds containing incorrect numbers of von Willebrand factor-positive granules. Tmod3(-/-) MKs exhibit F-actin disturbances, and Tmod3(-/-) MKs spreading on collagen fail to polymerize F-actin into actomyosin contractile bundles. Tmod3 associates with TM4 and the F-actin cytoskeleton in wild-type MKs, and confocal microscopy reveals that Tmod3, TM4, and F-actin partially colocalize near the membrane of proplatelet buds. In contrast, the abnormally large proplatelets from Tmod3(-/-) MKs show increased F-actin and redistribution of F-actin and TM4 from the cortex to the cytoplasm, but normal microtubule coil organization. We conclude that F-actin capping by Tmod3 regulates F-actin organization in mouse fetal liver-derived MKs, thereby controlling MK cytoplasmic morphogenesis, including DMS formation and organelle distribution, as well as proplatelet formation and sizing.
Asunto(s)
Citoesqueleto de Actina/patología , Plaquetas/patología , Membrana Celular/patología , Embrión de Mamíferos/patología , Hemorragia/etiología , Megacariocitos/patología , Tropomodulina/fisiología , Citoesqueleto de Actina/metabolismo , Animales , Apoptosis , Plaquetas/metabolismo , Western Blotting , Membrana Celular/metabolismo , Proliferación Celular , Células Cultivadas , Citoplasma/metabolismo , Embrión de Mamíferos/metabolismo , Femenino , Técnica del Anticuerpo Fluorescente , Hematopoyesis/fisiología , Hemorragia/metabolismo , Hemorragia/patología , Inmunoprecipitación , Megacariocitos/metabolismo , Ratones , Ratones Noqueados , Microscopía Confocal , Microscopía Electrónica de Transmisión , Microscopía Fluorescente , Ploidias , PolimerizacionRESUMEN
Nemaline myopathy is an inherited muscle disease that is mainly diagnosed by the presence of nemaline rods in muscle biopsies. Of the nine genes associated with the disease, five encode components of striated muscle sarcomeres. In a genetic zebrafish screen, the mutant träge (trg) was isolated based on its reduction in muscle birefringence, indicating muscle damage. Myofibres in trg appeared disorganised and showed inhomogeneous cytoplasmic eosin staining alongside malformed nuclei. Linkage analysis of trg combined with sequencing identified a nonsense mutation in tropomodulin4 (tmod4), a regulator of thin filament length and stability. Accordingly, although actin monomers polymerize to form thin filaments in the skeletal muscle of tmod4(trg) mutants, thin filaments often appeared to be dispersed throughout myofibres. Organised myofibrils with the typical striation rarely assemble, leading to severe muscle weakness, impaired locomotion and early death. Myofibrils of tmod4(trg) mutants often featured thin filaments of various lengths, widened Z-disks, undefined H-zones and electron-dense aggregations of various shapes and sizes. Importantly, Gomori trichrome staining and the lattice pattern of the detected cytoplasmic rods, together with the reactivity of rods with phalloidin and an antibody against actinin, is reminiscent of nemaline rods found in nemaline myopathy, suggesting that misregulation of thin filament length causes cytoplasmic rod formation in tmod4(trg) mutants. Although Tropomodulin4 has not been associated with myopathy, the results presented here implicateTMOD4 as a novel candidate for unresolved nemaline myopathies and suggest that the tmod4(trg) mutant will be a valuable tool to study human muscle disorders.
Asunto(s)
Mutación , Tropomodulina/genética , Tropomodulina/fisiología , Proteínas de Pez Cebra/genética , Proteínas de Pez Cebra/fisiología , Actinina/química , Actinas/química , Alelos , Animales , Animales Modificados Genéticamente , Citoplasma/metabolismo , Modelos Animales de Enfermedad , Ligamiento Genético , Masculino , Músculos/patología , Enfermedades Musculares/genética , Enfermedades Musculares/patología , Miofibrillas/metabolismo , Enfermedades Neuromusculares/metabolismo , Faloidina/química , Fenotipo , Sarcómeros/metabolismo , Pez CebraRESUMEN
Tropomodulins (Tmods) are F-actin pointed end capping proteins that interact with tropomyosins (TMs) and cap TM-coated filaments with higher affinity than TM-free filaments. Here, we tested whether differences in recognition of TM or actin isoforms by Tmod1 and Tmod3 contribute to the distinct cellular functions of these Tmods. We found that Tmod3 bound ~5-fold more weakly than Tmod1 to α/ßTM, TM5b, and TM5NM1. However, surprisingly, Tmod3 was as effective as Tmod1 at capping pointed ends of skeletal muscle α-actin (αsk-actin) filaments coated with α/ßTM, TM5b, or TM5NM1. Tmod3 only capped TM-coated αsk-actin filaments more weakly than Tmod1 in the presence of recombinant αTM2, which is unacetylated at its NH2 terminus, binds F-actin weakly, and has a disabled Tmod-binding site. Moreover, both Tmod1 and Tmod3 were similarly effective at capping pointed ends of platelet ß/cytoplasmic γ (γcyto)-actin filaments coated with TM5NM1. In the absence of TMs, both Tmod1 and Tmod3 had similarly weak abilities to nucleate ß/γcyto-actin filament assembly, but only Tmod3 could sequester cytoplasmic ß- and γcyto-actin (but not αsk-actin) monomers and prevent polymerization under physiological conditions. Thus, differences in TM binding by Tmod1 and Tmod3 do not appear to regulate the abilities of these Tmods to cap TM-αsk-actin or TM-ß/γcyto-actin pointed ends and, thus, are unlikely to determine selective co-assembly of Tmod, TM, and actin isoforms in different cell types and cytoskeletal structures. The ability of Tmod3 to sequester ß- and γcyto-actin (but not αsk-actin) monomers in the absence of TMs suggests a novel function for Tmod3 in regulating actin remodeling or turnover in cells.
Asunto(s)
Actinas/fisiología , Isoformas de Proteínas/fisiología , Tropomodulina/fisiología , Tropomiosina/fisiología , Actinas/metabolismo , Animales , Citoesqueleto/metabolismo , Músculo Esquelético/metabolismo , Músculo Esquelético/fisiología , Unión Proteica , Isoformas de Proteínas/metabolismo , Conejos , Sarcómeros/metabolismo , Espectrometría de Fluorescencia , Tropomodulina/metabolismo , Tropomiosina/metabolismoRESUMEN
Tmod4 (Tropomodulin 4) is a member of Tmod family that plays important role in thin filament length regulation and myofibril assembly. We found that the expression levels of Tmod4 were higher in skeletal muscle and adipose tissues. However, the function and regulation of the Tmod4 gene in the myogenesis and adipogenesis remains unclear. In this study, we found that the expression of Tmod4 was decreased in myogenesis while increased in adipogenesis. Then, the transcriptional regulation analysis of Tmod4 promoter showed that Tmod4 could be regulated directly by myogenic factors and adipogenic factors. Furthermore, the roles of Tmod4 in the myogenesis and adipogenesis were confirmed by its over-expression in C2C12 cells and 3T3 cells, which suggested that Tmod4 could promote adipogenesis by up-regulating the adipogenic factors but moderately delay the myogenesis. These results indicated that the Tmod4 gene may play as a switch between myogenesis and adipogenesis, which resulted in the balanced development between skeletal muscle and adipose tissue. Therefore, the model for switch role of the Tmod4 in the balanced regulation between myogenesis and adipogenesis was proposed. It is showed that the expression of Tmod4 was activated in adipogenesis by adipogenic factors while inhibited in myogenesis by myogenic factors. Moreover, Tmod4 could promote adipogenesis by up-regulating the expression of adipogenic factors while moderately delaying the myogenesis. Our study provides an important basis for further understanding the regulation and function of porcine Tmod4 in muscle and fat development.
Asunto(s)
Adipogénesis/genética , Desarrollo de Músculos/genética , Sus scrofa/genética , Tropomodulina/fisiología , Células 3T3 , Tejido Adiposo/crecimiento & desarrollo , Animales , Secuencia de Bases , Sitios de Unión , Secuencia Conservada , Regulación del Desarrollo de la Expresión Génica , Ratones , Datos de Secuencia Molecular , Especificidad de Órganos , Unión Proteica , Transporte de Proteínas , Análisis de Secuencia de ADN , Sus scrofa/crecimiento & desarrollo , Transcripción GenéticaRESUMEN
The basis for mammalian lens fiber cell organization, transparency, and biomechanical properties has contributions from two specialized cytoskeletal systems: the spectrin-actin membrane skeleton and beaded filament cytoskeleton. The spectrin-actin membrane skeleton predominantly consists of α2ß2-spectrin strands interconnecting short, tropomyosin-coated actin filaments, which are stabilized by pointed-end capping by tropomodulin 1 (Tmod1) and structurally disrupted in the absence of Tmod1. The beaded filament cytoskeleton consists of the intermediate filament proteins CP49 and filensin, which require CP49 for assembly and contribute to lens transparency and biomechanics. To assess the simultaneous physiological contributions of these cytoskeletal networks and uncover potential functional synergy between them, we subjected lenses from mice lacking Tmod1, CP49, or both to a battery of structural and physiological assays to analyze fiber cell disorder, light scattering, and compressive biomechanical properties. Findings show that deletion of Tmod1 and/or CP49 increases lens fiber cell disorder and light scattering while impairing compressive load-bearing, with the double mutant exhibiting a distinct phenotype compared to either single mutant. Moreover, Tmod1 is in a protein complex with CP49 and filensin, indicating that the spectrin-actin network and beaded filament cytoskeleton are biochemically linked. These experiments reveal that the spectrin-actin membrane skeleton and beaded filament cytoskeleton establish a novel functional synergy critical for regulating lens fiber cell geometry, transparency, and mechanical stiffness.
Asunto(s)
Proteínas del Ojo/fisiología , Proteínas de Filamentos Intermediarios/fisiología , Cristalino/citología , Tropomodulina/fisiología , Actinas/metabolismo , Animales , Fenómenos Biomecánicos , Forma de la Célula , Citoesqueleto/fisiología , Citoesqueleto/ultraestructura , Proteínas del Ojo/genética , Proteínas del Ojo/metabolismo , Proteínas de Filamentos Intermediarios/genética , Proteínas de Filamentos Intermediarios/metabolismo , Cristalino/fisiología , Cristalino/ultraestructura , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos , Mutagénesis Sitio-Dirigida , Óptica y Fotónica , Tropomodulina/genética , Tropomodulina/metabolismoRESUMEN
Lens fiber cells exhibit a high degree of hexagonal packing geometry, determined partly by tropomodulin 1 (Tmod1), which stabilizes the spectrin-actin network on lens fiber cell membranes. To ascertain whether Tmod1 is required during epithelial cell differentiation to fiber cells or during fiber cell elongation and maturation, the authors quantified the extent of fiber cell disorder in the Tmod1-null lens and determined locations of disorder by confocal microscopy and computational image analysis. First, nearest neighbor analysis of fiber cell geometry in Tmod1-null lenses showed that disorder is confined to focal patches. Second, differentiating epithelial cells at the equator aligned into ordered meridional rows in Tmod1-null lenses, with disordered patches first observed in elongating fiber cells. Third, as fiber cells were displaced inward in Tmod1-null lenses, total disordered area increased due to increased sizes (but not numbers) of individual disordered patches. The authors conclude that Tmod1 is required first to coordinate fiber cell shapes and interactions during tip migration and elongation and second to stabilize ordered fiber cell geometry during maturation in the lens cortex. An unstable spectrin-actin network without Tmod1 may result in imbalanced forces along membranes, leading to fiber cell rearrangements during elongation, followed by propagation of disorder as fiber cells mature.
Asunto(s)
Cristalino/citología , Tropomodulina/fisiología , Actinas/ultraestructura , Animales , Recuento de Células , Diferenciación Celular , Membrana Celular/ultraestructura , Movimiento Celular , Forma de la Célula , Células Epiteliales/citología , Cristalino/metabolismo , Ratones , Ratones Noqueados , Tropomodulina/genéticaRESUMEN
Erythrocyte tropomodulin (E-Tmod) is first isolated from human erythrocyte membrane as a TM-binding protein. Its N-terminus contains two TM-binding sites and one TM-dependent actin capping domain and C-terminus contains 5 leucine-rich repeats and a TM-independent actin capping domain. As the unique capping protein at the slow-growing end of F-actin, E-Tmod binds to N-terminus of TM and actin and decreases the TM-coated F-actin depolymerization. E-Tmod encoding gene is highly conserved and E-Tmod is distributed widely in erythrocytes and cardiomyocytes, etc. E-Tmod plays a pivotal role in organizing F-actin and cytoskeleton and maintaining the mechanical properties of the cells.
Asunto(s)
Proteínas de Capping de la Actina/fisiología , Citoesqueleto de Actina/fisiología , Tropomodulina/fisiología , Animales , Citoesqueleto/fisiología , Humanos , Tropomiosina/fisiologíaRESUMEN
Erythrocyte tropomodulin (E-Tmod) is a tropomyosin-binding and actin capping protein at the point end of the filaments. It is part of a molecular ruler that plays an important role in generating short actin protofilaments critical for the integrity of the cell membrane. Here, with the use of E-Tmod+/lacZ mice, we demonstrated a specific E-Tmod expression in horizontal cells (HCs) in the retina, and analyzed the stress-strain relationship of HCs, vertically oriented neurons, and retinal ganglial cells (RGC) under normal and high intraocular pressure (IOP). Since their dendrites are oriented laterally in a plane and form most complicated synapses with multiple cone photoreceptors, HCs are subjected to a greater stress and strain than vertically oriented neurons. The specific E-Tmod expression suggests its role in protecting HCs from mechanical damages in certain eye diseases, such as glaucoma, a neurodegenerative disease of the retina characterized by an elevated IOP. A stress-strain analysis on axons of RGC that run horizontally but only anchor at the optical nerve head suggests that they may also be subjected to a higher mechanical stress, which leads to an increase in "cup-to-disc" ratio in a higher IOP or in glaucoma patients.
Asunto(s)
Glaucoma/patología , Células Horizontales de la Retina , Tropomodulina/fisiología , Animales , Axones/patología , Axones/fisiología , Fenómenos Biomecánicos/fisiología , Dendritas/patología , Dendritas/fisiología , Glaucoma/fisiopatología , Presión Intraocular/fisiología , Ratones , Ratones Noqueados , Disco Óptico/patología , Disco Óptico/fisiopatología , Células Fotorreceptoras de Vertebrados/patología , Células Fotorreceptoras de Vertebrados/fisiología , Células Bipolares de la Retina/patología , Células Bipolares de la Retina/fisiología , Células Ganglionares de la Retina/patología , Células Ganglionares de la Retina/fisiología , Células Horizontales de la Retina/metabolismo , Células Horizontales de la Retina/patología , Estrés MecánicoRESUMEN
Dynamics of the slow-growing (pointed) end of the actin filament is regulated by tropomodulins, a family of capping proteins that require tropomyosin for optimal function. Tropomodulin is an elongated molecule with a molecular mass of about 40 kDa, containing the Tm-independent actin-binding site at the C-terminus. The highly disordered N-terminal half of tropomodulin contains two Tm-binding sites and a Tm-dependent actin-binding site. There are many Tm isoforms whose distribution varies in different tissues and cell compartments and changes during development of these tissues. Tropomyosin/tropomodulin interactions are isoform specific. Differences in Tm affinity for the two binding sites in Tmod may regulate its correct positioning at the pointed end as well as effectiveness of capping actin filament. The regulation of tropomodulin binding may have significant consequences for local cytoskeletal formation and filament dynamics in cells.
Asunto(s)
Actinas/química , Tropomodulina/fisiología , Tropomiosina/fisiología , Secuencia de Aminoácidos , Animales , Sitios de Unión , Humanos , Modelos Moleculares , Datos de Secuencia Molecular , Conformación Proteica , Isoformas de Proteínas , Estructura Secundaria de Proteína , Estructura Terciaria de Proteína , Homología de Secuencia de Aminoácido , Tropomodulina/química , Tropomodulina/metabolismo , Tropomiosina/químicaRESUMEN
Regulation of the actin cytoskeleton by filament capping proteins is critical to myriad dynamic cellular functions. The ability of these proteins to bind both filaments as well as monomers is often central to their cellular functions. The ubiquitous pointed end capping protein Tmod3 (tropomodulin 3) acts as a negative regulator of cell migration, yet mechanisms behind its cellular functions are not understood. Analysis of Tmod3 effects on kinetics of actin polymerization and steady state monomer levels revealed that Tmod3, unlike previously characterized tropomodulins, sequesters actin monomers with an affinity similar to its affinity for capping pointed ends. Furthermore, Tmod3 is found bound to actin in high speed supernatant cytosolic extracts, suggesting that Tmod3 can bind to monomers in the context of other cytosolic monomer binding proteins. The Tmod3-actin complex can be efficiently cross-linked with 1-ethyl-3-(dimethylaminopropyl)carbodiimide/N-hydroxylsulfosuccinimide in a 1:1 complex. Subsequent tryptic digestion and liquid chromatography/tandem mass spectrometry revealed two binding interfaces on actin, one distinct from other actin monomer binding proteins, and two potential binding sites in Tmod3, which are independent of the previously characterized leucine-rich repeat structure involved in pointed end capping. These data suggest that the Tmod3 isoform may regulate actin dynamics differently in cells than the previously described tropomodulin isoforms.