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To support training and foster retention in the emerging field of astrobiology, NASA has funded opportunities for graduate students and early career scientists to develop a community, foster interdisciplinarity, increase confidence, and showcase career options. The design of these opportunities builds on research on factors that increase retention, including feeling competent, having autonomy and a sense of purpose, having a sense of identity, and being connected to others in the field. Findings are reported from retrospective studies of two NASA career-building opportunities, the Astrobiology Graduate Conference and the International Astrobiology Summer School held in Santander, Spain. We present evidence that attendees gain confidence by presenting to, and working with, their peers, and feel competent to express their ideas and interests and build relationships in the field that continue after the experiences. Many say that they feel less isolated and go on to present or publish with colleagues they meet. Their career options also expand by meeting potential colleagues from different disciplines. Based on the findings, participating in either of these long-running programs shows clear positive impact on early career astrobiology professionals.
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Selección de Profesión , Exobiología , Exobiología/educación , Estados Unidos , United States National Aeronautics and Space Administration , HumanosRESUMEN
Fit and accommodation are critical design goals for a body armor system to maximize Soldiers' protection, comfort, mobility, and performance. The aim of this study is to assess fit and accommodation of body armor plates for the US Army. A virtual fit assessment technique, developed, validated, and deployed by NASA for spacesuit design, was adopted for this work. Specifically, 3D manikins of the Soldier population were overlaid virtually with geometrically similar surrogates of the armor plates. Trained subject matter experts with the US Army and NASA manually assessed the fit of the armor plates to manikins using a computer visualization tool and selected the appropriate plate size and position. A prediction model was built from the assessment data to predict the plate size from an arbitrary body shape and the resultant patterns of body-to-plate contact were quantified. The outcome indicated a unique trend of the plate sizes covarying with anthropometry. More pronouncedly, when the overlap between the body tissue and armor plate was quantified, female Soldiers are likely to experience a 25 times larger body-to-plate contact volume and 6.5 times larger contact depth than males on average, due to sex-based anthropometric differences. Overall, the prediction model and contact patterns provided key metrics for virtual body armor fit assessments, of which the locations, patterns, and magnitudes can help to improve sizing and fit of body armor systems, as previously demonstrated for NASA spacesuit design.
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Diseño de Equipo , Maniquíes , Personal Militar , United States National Aeronautics and Space Administration , Humanos , Masculino , Femenino , Estados Unidos , Antropometría/métodos , Adulto , Ropa de Protección , Trajes EspacialesRESUMEN
Animal agriculture is under pressure to increase efficiency, sustainability, and innovation to meet the demands of a rising global population while decreasing adverse environmental effects. Feed cost and availability are 2 of the biggest hurdles to sustainable production. Current diets depend on sources of grain and animal byproduct protein for essential amino acids which have limited sustainability. Insects have arisen as an attractive, sustainable alternative protein source for animal diets due to their favorable nutrient composition, low space and water requirements, and natural role in animal diets. Additionally, insects are capable of bioremediating waste streams including agricultural and food waste, manure, and plastics helping to increase their sustainability. The insect rearing industry has grown rapidly in recent years and shows great economic potential. However, state-of-the-art research is urgently needed to overcome barriers to adoption in commercial animal diets such as regulatory restrictions, production scale issues, and food safety concerns. To address this need, the USDA Agricultural Research Service "MINIstoc: Model for INsect Inclusion" project was created to bring together diverse scientists from across the world to synergistically advance insect meal production and inclusion in animal diets. Here, we provide a short review of insects as feed while describing the MINIstock project which serves as the inspiration for the Journal of Economic Entomology Special Collection "Insects as feed: sustainable solutions for food waste and animal production practices."
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Agricultura , Alimentación Animal , Insectos , United States Department of Agriculture , Animales , Alimentación Animal/análisis , Estados Unidos , Agricultura/métodos , Dieta , United States National Aeronautics and Space Administration , Crianza de Animales DomésticosRESUMEN
The operational environment of complex sociotechnical systems is inherently uncertain, demanding constant coordination restructuring to adapt to dynamic situational demands. However, coordination changes in the Human Factors and Ergonomics Field have primarily been studied using static methods, overlooking moment-by-moment adjustments. In the current study, we address coordination restructuring by using THEME, a digital analytical tool capable of visualising and exploring coordination restructuring from a multi-layered perspective. We examine restructuring in coordination patterns during NASA's Apollo 13 Mission, revealing significant shifts from stable, long-duration 'coordination hubs' in routine operations to shorter-duration patterns during a crisis situation. Additionally, the results highlight the importance of flexible switching between reciprocal and one-directed coordination, along with enhanced role distribution. This study underscores how exploring temporality-sensitive phenomena like coordination through digital technologies such as THEME, advances our understanding of incident analysis and resilient performance within complex systems.
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Ergonomía , Humanos , Ergonomía/métodos , Estados Unidos , United States National Aeronautics and Space Administration , Vuelo Espacial , Ciencia de los Datos/métodos , Análisis y Desempeño de Tareas , Tecnología DigitalRESUMEN
INTRODUCTION: This paper describes the method for assigning medical diagnostic and treatment capabilities and resources to the database which assists with an updated probabilistic risk analysis (PRA) tool for exploration class medical system planning. The National Aeronautics and Space Administration has used PRA since 2011 to inform mission medical system design, but existing tools are designed only for low Earth orbit. An updated PRA tool was needed to assist with exploration class missions.METHODS: A team of medical experts with a wide range of expertise and experience, including Space Medicine, was assembled to build capability and resource tables for the new PRA tool. This team met over 8 mo and used practice guidelines, literature, and experience to build capability and resource tables (CRTs) for each condition in the new PRA tool database.RESULTS: This process led to CRTs for each condition and a total of 617 distinct capabilities and 839 discrete resources.CONCLUSION: The CRT method is an effective way to translate medical practice guidelines into capabilities and resources usable by PRA tools for exploration class medical system planning. This same method may be used in commercial space ventures and in other applications in which medical predictive analytics are informative.Levin DR, Nelson AM, Zahner C, Stratton ER, Anderson A, Steller J. A method to determine capabilities and resources for spacecraft medical systems. Aerosp Med Hum Perform. 2024; 95(7):403-408.
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Medicina Aeroespacial , Vuelo Espacial , Nave Espacial , Humanos , Medicina Aeroespacial/métodos , Estados Unidos , Medición de Riesgo/métodos , United States National Aeronautics and Space AdministrationRESUMEN
BACKGROUND: In the early days of the National Aeronautics and Space Administration (NASA), medicine in support of the astronauts was led by military experts from the U.S. Air Force as well as experts from the U.S. Navy and U.S. Army. In the early years, a physician with expertise in aerospace medicine was assigned to the Space Task Group and then to NASA. One of these individuals was Dr. Stanley White, a U.S. Air Force physician. To capture more of the early space medicine pioneers, a contract was established between the National Library of Medicine and the principal investigator at the University of Cincinnati to conduct a series of interviews with these early pioneers. An interview with Dr. White took place in his home while he was in hospice care. This audiotaped interview and other written and oral histories within NASA archives and the literature were reviewed to support this work. A series of questions were prepared for the interaction with Dr. White. These questions provided further clarification on his background and contribution. Responses to questions elicited open-ended discussion. The conversation provided a historical summary of Dr. White's contribution to NASA as one of its first flight surgeons.Doarn CR. An interview with Dr. Stanley White, one of NASA's first flight surgeons. Aerosp Med Hum Perform. 2024; 95(4):223-225.
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Medicina Aeroespacial , Vuelo Espacial , Cirujanos , Astronautas , Estados Unidos , United States National Aeronautics and Space AdministrationRESUMEN
The efficacy of the NASA SPRINT exercise countermeasures program for quadriceps (vastus lateralis) and triceps surae (soleus) skeletal muscle health was investigated during 70 days of simulated microgravity. Individuals completed 6° head-down-tilt bedrest (BR, n = 9), bedrest with resistance and aerobic exercise (BRE, n = 9), or bedrest with resistance and aerobic exercise and low-dose testosterone (BRE + T, n = 8). All groups were periodically tested for muscle (n = 9 times) and aerobic (n = 4 times) power during bedrest. In BR, surprisingly, the typical bedrest-induced decrements in vastus lateralis myofiber size and power were either blunted (myosin heavy chain, MHC I) or eliminated (MHC IIa), along with no change (P > 0.05) in %MHC distribution and blunted quadriceps atrophy. In BRE, MHC I (vastus lateralis and soleus) and IIa (vastus lateralis) contractile performance was maintained (P > 0.05) or increased (P < 0.05). Vastus lateralis hybrid fiber percentage was reduced (P < 0.05) and energy metabolism enzymes and capillarization were generally maintained (P > 0.05), while not all of these positive responses were observed in the soleus. Exercise offsets 100% of quadriceps and approximately two-thirds of soleus whole muscle mass loss. Testosterone (BRE + T) did not provide any benefit over exercise alone for either muscle and for some myocellular parameters appeared detrimental. In summary, the periodic testing likely provided a partial exercise countermeasure for the quadriceps in the bedrest group, which is a novel finding given the extremely low exercise dose. The SPRINT exercise program appears to be viable for the quadriceps; however, refinement is needed to completely protect triceps surae myocellular and whole muscle health for astronauts on long-duration spaceflights.NEW & NOTEWORTHY This study provides unique exercise countermeasures development information for astronauts on long-duration spaceflights. The NASA SPRINT program was protective for quadriceps myocellular and whole muscle health, whereas the triceps surae (soleus) was only partially protected as has been shown with other programs. The bedrest control group data may provide beneficial information for overall exercise dose and targeting fast-twitch muscle fibers. Other unique approaches for the triceps surae are needed to supplement existing exercise programs.
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Ejercicio Físico , Músculo Esquelético , Cadenas Pesadas de Miosina , Músculo Cuádriceps , Simulación de Ingravidez , Humanos , Masculino , Músculo Cuádriceps/fisiología , Músculo Cuádriceps/metabolismo , Simulación de Ingravidez/métodos , Adulto , Ejercicio Físico/fisiología , Cadenas Pesadas de Miosina/metabolismo , Músculo Esquelético/fisiología , Músculo Esquelético/metabolismo , United States National Aeronautics and Space Administration , Estados Unidos , Reposo en Cama/efectos adversos , Testosterona/metabolismo , Testosterona/sangre , Vuelo Espacial/métodos , Atrofia Muscular/prevención & control , Atrofia Muscular/fisiopatología , Entrenamiento de Fuerza/métodos , Ingravidez/efectos adversos , Fuerza Muscular/fisiologíaRESUMEN
BACKGROUND: Perceived surgeon workload of performing primary and revision total hip arthroplasty (THA) and total knee arthroplasty (TKA) is challenging to quantify. The National Aeronautics and Space Administration Task Load Index (NASA TLX) survey was developed to quantify experiences following aviation and has been applied to healthcare fields. Our purposes were to 1) quantify the workload endured by surgeons who are performing primary and revision TKA and THA and 2) compare these values to their Center for Medicare & Medicaid Services (CMS) reimbursement. METHODS: A prospective cohort of 5 fellowship-trained adult reconstruction surgeons completed NASA TLX surveys following primary and revision TKA/THA cases. A total of 122 surveys consisting of 70 TKA (48 primaries and 22 revisions) and 55 THA surveys (38 primaries and 17 revisions) were completed. Patient demographics and surgical variables were recorded. Final NASA TLX workloads were compared to 2021 CMS work relative value units. RESULTS: Compared to primary TKA, revision TKA had 176% increased intraoperative workload (P < .001), 233% increased mental burden (P < .001), and 150% increased physical burden (P < .001). Compared to primary THA, revision THA had 106% increased intraoperative workload (P < .001), 96% increased mental burden (P < .001), and 91% increased physical burden (P < .001). Operative time was higher in revision versus primary TKA (118 versus 84.5 minutes, P = .05) and THA (150 versus 115 minutes, P = .001). Based upon 2021 CMS data, revision TKA and THA would need to be compensated by an additional 36% and 12.3%, respectively, to parallel intraoperative efforts. CONCLUSIONS: Revision hip and knee arthroplasty places a major mental and physical workload upon surgeons and is disproportionately compensated by CMS.
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Artroplastia de Reemplazo de Cadera , Artroplastia de Reemplazo de Rodilla , Reoperación , Carga de Trabajo , Humanos , Carga de Trabajo/estadística & datos numéricos , Artroplastia de Reemplazo de Cadera/estadística & datos numéricos , Artroplastia de Reemplazo de Rodilla/estadística & datos numéricos , Masculino , Femenino , Reoperación/estadística & datos numéricos , Estados Unidos , Estudios Prospectivos , Persona de Mediana Edad , Anciano , United States National Aeronautics and Space Administration , Cirujanos/estadística & datos numéricos , Encuestas y Cuestionarios , AdultoRESUMEN
The National Aeronautics and Space Administration (NASA) has recently made a long-term commitment towards fostering open science. The NASA Transform to Open Science (TOPS) initiative provides recommendations, best practices, and tools related to open science. The principles of open science include the transparent sharing of data, findings, and methods and is designed to accelerate the pace of discovery and foster collaboration. The goal of open science is to allow data, publications, software, and physical samples to be accessible to all, regardless of being a professional or an amateur. In this paper, we summarize several key points open science that were presented as part of NASA's Open Science 101 Module 1 at an in-person training event in Washington, D.C., and include how open science can be beneficial for researchers and society as a whole.
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Medicina Aeroespacial , United States National Aeronautics and Space Administration , Humanos , Estados Unidos , Curriculum , Difusión de la Información/métodosRESUMEN
Complex assembly tasks with multiple manual operations and steps often require rapid judgment and action under time pressure and cause most human-related errors. The task switching and action transitions are major sources of these errors. This study intends to implement an electroencephalography (EEG) approach to quantitatively evaluate the mental workload during task switching and transition. The time-frequency and spectrum analysis were utilized to compute and reflect the task demand between the intervals of individual tasks. This study developed an experiment to validate the proposed assessment approach and benchmark the results with the National Aeronautics and Space Administration task load index (NASA-TLX) subjective evaluation scale analysis. The results show that the average value of the power spectral densities (PSDs) of the gamma band signal of the AF4 channel and the beta band signal of Channel F3 show distinctive signal patterns among task stages and intervals. During the interval between the idling stage and the part selection stage, the peak of the PSD envelope increased from 18 to 27 Hz, suggesting advanced cognition increases the mental workload of the interval between different tasks. Therefore, the task switching period cannot be regarded as rest and need to be optimized with better task organization.
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Análisis y Desempeño de Tareas , Carga de Trabajo , Estados Unidos , Humanos , Cognición , Juicio , United States National Aeronautics and Space AdministrationRESUMEN
Heat stress is a prominent issue in livestock production, even for intensively housed dairy herds in Canada. Production records and meteorological data can be combined to assess heat tolerance in dairy cattle. The overall aim of this study was to evaluate the possibility of genetic evaluation for heat tolerance in Canadian dairy cattle. The 2 specific objectives were (1) to estimate the genetic parameters for milk, fat, and protein yield for Holsteins while accounting for high environmental heat loads, and (2) to determine if a genotype-by-environment interaction causes reranking of top-ranked sires between environments with low and high heat loads. A repeatability test-day model with a heat stress function was used to evaluate the genetic merit for milk, fat, and protein yield under heat stress and at thermal comfort for first parity in 5 regions in Canada. The heat stress function for each trait was defined using a specific temperature-humidity index (THI) threshold. The purpose of this function was to quantify the level of heat stress that was experienced by the dairy cattle. The estimated genetic correlation between the general additive genetic effect and the additive effect on the slope of the change in the trait phenotype for milk, fat, and protein yield ranged from -0.16 to -0.30, -0.20 to -0.44, and -0.28 to -0.42, respectively. These negative correlations imply that there is an antagonistic relationship between sensitivity to heat stress and level of production. The heritabilities for milk, fat, and protein yield at 15 units above the THI threshold ranged from 0.15 to 0.27, 0.11 to 0.15, and 0.11 to 0.15, respectively. Finally, the rank correlations between the breeding values from a repeatability model with no heat stress effect and the breeding values accounting for heat stress for the 100 top-ranked bulls indicated possible interaction between milk production traits and THI, resulting in substantial reranking of the top-ranked sires in Canada, especially for milk yield. This is the first study to implement weather data from the NASA POWER database in a genetic evaluation of heat tolerance in dairy cattle. The NASA POWER database is a novel alternative meteorological resource that is potentially more reliable and consistent and with broader coverage than weather station data increasing the number of animals that could be included in a heat stress evaluation.
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Enfermedades de los Bovinos , Trastornos de Estrés por Calor , Termotolerancia , Embarazo , Femenino , Estados Unidos , Masculino , Bovinos/genética , Animales , Termotolerancia/genética , Lactancia/genética , United States National Aeronautics and Space Administration , Calor , Canadá , Tiempo (Meteorología) , Leche/metabolismo , Humedad , Trastornos de Estrés por Calor/veterinaria , Enfermedades de los Bovinos/metabolismoRESUMEN
As part of planning for future space exploration, COSPAR (The Committee on Space Research) together with participating space agencies, organized and held interdisciplinary meetings to consider next steps in addressing knowledge gaps for planetary protection for future human missions to Mars. Beginning with the results of these meetings and earlier work by NASA, ESA, and COSPAR (e.g., Criswell et al., 2005; Hogan et al., 2006; Rummel et al., 2008) as a base the authors of this paper carried out a follow-on NASA planning activity to identify the necessary steps to be accomplished to close knowledge gaps. We identified significant overlap between the planetary protection needs and other sets of Mars preparation roadmaps (1) microbial monitoring requirements for crew health and medical systems, (2) studies of the microbiome of the built environment, (3) environmental control and life support systems (ECLSS), (4) waste management, and (5) planetary surface operations. In many cases, efforts to mature exploration class systems for Mars that are occurring in other domains can be leveraged with minor changes to address planetary protection gaps as well. In other cases, work planned for testing on the International Space Station (ISS) as an analog for crew Mars transit, or on the lunar surface as an analog for Mars surface operations can be used to close planetary protection technology and knowledge gaps. An overall strategic framework that combines these domains has the advantage of being more comprehensive, efficient, and timely for closing gaps. This approach has led to the development of a NASA roadmap for addressing planetary protection integrated with other related roadmaps. NASA's development and execution of the planetary protection is now viewed in an integrated way with related technology development and testing. Key features of the integrated capabilities roadmap include.
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Marte , Microbiota , Vuelo Espacial , Estados Unidos , Humanos , United States National Aeronautics and Space Administration , LunaRESUMEN
BioSentinel is a nanosatellite deployed from Artemis-I designed to conduct in-situ biological measurements on yeast cells in the deep space radiation environment. Along with the primary goal of measuring damage and response in cells exposed during spaceflight, on-board active dosimetry will provide measurements of the radiation field encountered behind moderate shielding provided by the BioSentinel housing and internal components. The measurements are particularly important to enable interpretation of biological observations but also provide an opportunity to validate integrated computational models used to calculate radiation environments. In this work, models are used to predict the galactic cosmic ray exposure anticipated for the BioSentinel payload and on-board dosimeter. The model calculations presented herein were completed prior to the Artemis-I launch on November 16, 2022, and therefore represent actual predictions (i.e., unbiased by a priori knowledge of on-board measurements). Such time-forward predictions are rarely performed for space radiation applications due to limitations of environmental models, but truly independent model validation will be possible in the future when on-board measurements become available. The method used to facilitate future projections within an existing GCR (galactic cosmic ray) environmental model is described, and projection uncertainties are quantified and contextualized.
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Radiación Cósmica , Vuelo Espacial , Estados Unidos , United States National Aeronautics and Space Administration , Radiometría , Modelos TeóricosRESUMEN
The relationship framework between electroencephalogram (EEG) signals and subjective perception during pilots' missions was established to improve pilots' training efficiency and flight safety. Primarily, this study constructs a real flight scene through virtual reality (VR) and then obtains EEG data in simulated scenes. Researchers use VR technology to build a mission simulation room and then acquire EEG data from participants wearing EEG acquisition devices in the simulated room. The experimental process is divided into flight simulation and a questionnaire survey. Based on the participants' EEG analysis, the researchers verified the changes of ß rhythm under a high-difficulty operation mission. In addition, this study infers the mechanism of affecting pilots' mental workload under high-difficulty operation by analyzing the correlation between subjective questionnaire results and ß rhythms. The results showed that in the context of pilots performing flight missions in the aircraft space environment, the pilots' mental load had the most excellent rhythmic relationship with the regions representing ß rhythm. Therefore, a comprehensive experimental framework constructed in this study based on virtual simulation space to analyze the relationship between EEG and NASA-TLX subjective questionnaire provides a set of more accurate reference information for the design of a pilot training system based on pilot training efficiency and flight safety.
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United States National Aeronautics and Space Administration , Realidad Virtual , Estados Unidos , Humanos , Aeronaves , Carga de Trabajo , ElectroencefalografíaRESUMEN
Abstract Responsible space exploration is a cornerstone of planetary protection, particularly at sites in the Solar System with a high potential for the existence of extant life. To limit bioburden, spacecraft assembly occurs in cleanroom facilities. Cleanroom levels are established through air particulate counters that can assess particle size distribution and concentration but cannot detect bioaerosols. Additionally, these devices do not detect in real-time, which poses a risk to critical flight hardware assemblies or even mission timelines. A first-of-its-kind study was conducted to simultaneously detect bioaerosols, inert particles, and their size distribution in real-time in operational spacecraft assembly cleanrooms at NASA's Jet Propulsion Laboratory in Pasadena, CA, USA, using the BioVigilant IMD-A® 350 (Azbil Corporation, Tucson, AZ, USA). The IMD-350A continuously sampled during operations and no-operation 6 h intervals in two facilities per cleanroom class: ISO 6, ISO 7, and ISO 8. A positive correlation was established between human presence in the cleanroom and elevated bioaerosol counts. Smaller particles of sizes 0.5 and 1 µm constituted an average â¼91% of the total bioaerosols detected in At Work intervals across all ISO classes observed. The results of this study were used to establish bioburden particulate thresholds for the most stringent JPL cleanrooms used in the assembly of the Sample Caching System for the Mars 2020 Perseverance rover.
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Vuelo Espacial , Nave Espacial , Estados Unidos , Humanos , United States National Aeronautics and Space Administration , Ambiente Controlado , Planetas , PolvoRESUMEN
Heat stress negatively affects livestock, with undesirable effects on animals' production and reproduction. Temperature and humidity index (THI) is a climatic variable used worldwide to study the effect of heat stress on farm animals. Temperature and humidity data can be obtained in Brazil through the National Institute of Meteorology (INMET), but complete data may not be available due to temporary failures on weather stations. An alternative to obtaining meteorological data is the National Aeronautics and Space Administration Prediction of Worldwide Energy Resources (NASA POWER) satellite-based weather system. We aimed to compare THI estimates obtained from INMET weather stations and NASA POWER meteorological information sources using Pearson correlation and linear regression. After quality check, data from 489 INMET weather stations were used. The hourly, average daily and maximum daily THI were evaluated. We found greater correlations and better regression evaluation metrics when average daily THI values were considered, followed by maximum daily THI, and hourly THI. NASA POWER satellite-based weather system is a suitable tool for obtaining the average and maximum THI values using information collected from Brazil, showing high correlations with THI estimates from INMET and good regression evaluation metrics, and can assist studies that aim to analyze the impact of heat stress on livestock production in Brazil, providing additional data to complement the existing information available in the INMET database.
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Trastornos de Estrés por Calor , Meteorología , Animales , Estados Unidos , Femenino , Humedad , Temperatura , Brasil , United States National Aeronautics and Space Administration , Tiempo (Meteorología) , Trastornos de Estrés por Calor/veterinaria , Calor , Lactancia , LecheRESUMEN
Since the advent of space exploration missions, various space agencies have been working to improve the quality of food and nutrition for crew members. Food processing, preservation, and packaging have evolved with the advancement of technology. Most of the food available on earth can be consumed in space by changing its form. Shelf life and food acceptability can be enhanced by using suitable packaging materials. Here we review space food, which has been categorized into bite-size food, rehydratable food, thermostabilized food, intermediate moisture food, and irradiated food. Additionally, packaging materials and different packaging forms for space food are reviewed. Finally, the review highlights the challenges in space food packaging and food packaging trends of the Defence research and development organization (DRDO), the Japan aerospace exploration agency (JAXA), and the National aeronautics and space administration (NASA).
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Embalaje de Alimentos , Vuelo Espacial , Estados Unidos , Alimentos , Manipulación de Alimentos , Estado Nutricional , Astronautas , United States National Aeronautics and Space AdministrationRESUMEN
Molecular biology methods and technologies have advanced substantially over the past decade. These new molecular methods should be incorporated among the standard tools of planetary protection (PP) and could be validated for incorporation by 2026. To address the feasibility of applying modern molecular techniques to such an application, NASA conducted a technology workshop with private industry partners, academics, and government agency stakeholders, along with NASA staff and contractors. The technical discussions and presentations of the Multi-Mission Metagenomics Technology Development Workshop focused on modernizing and supplementing the current PP assays. The goals of the workshop were to assess the state of metagenomics and other advanced molecular techniques in the context of providing a validated framework to supplement the bacterial endospore-based NASA Standard Assay and to identify knowledge and technology gaps. In particular, workshop participants were tasked with discussing metagenomics as a stand-alone technology to provide rapid and comprehensive analysis of total nucleic acids and viable microorganisms on spacecraft surfaces, thereby allowing for the development of tailored and cost-effective microbial reduction plans for each hardware item on a spacecraft. Workshop participants recommended metagenomics approaches as the only data source that can adequately feed into quantitative microbial risk assessment models for evaluating the risk of forward (exploring extraterrestrial planet) and back (Earth harmful biological) contamination. Participants were unanimous that a metagenomics workflow, in tandem with rapid targeted quantitative (digital) PCR, represents a revolutionary advance over existing methods for the assessment of microbial bioburden on spacecraft surfaces. The workshop highlighted low biomass sampling, reagent contamination, and inconsistent bioinformatics data analysis as key areas for technology development. Finally, it was concluded that implementing metagenomics as an additional workflow for addressing concerns of NASA's robotic mission will represent a dramatic improvement in technology advancement for PP and will benefit future missions where mission success is affected by backward and forward contamination.