Hydrogen sulfide protects H9c2 cardiomyoblasts against H2O2-induced apoptosis
Rev. bras. pesqui. méd. biol
; Braz. j. med. biol. res;52(4): e7626, 2019. graf
Article
en En
| LILACS
| ID: biblio-1001516
Biblioteca responsable:
BR1.1
ABSTRACT
Reactive oxygen species (ROS) are highly reactive chemical species that may cause irreversible tissue damage, and play a critical role in cardiovascular diseases. Hydrogen sulfide (H2S) is a gasotransmitter that acts as a ROS scavenger with cardio-protective effects. In this study, we investigated the cytoprotective effect of H2S against H2O2-induced apoptosis in cardiomyocytes. H9c2 rat cardiomyoblasts were treated with H2S (100 μM) 24 h before challenging with H2O2 (100 μM). Apoptosis was then assessed by annexin V and PI, and mitochondrial membrane potential was measured using a fluorescent probe, JC-1. Our results revealed that H2S improved cell viability, reduced the apoptotic rate, and preserved mitochondrial membrane potential. An increased Bcl-2 to Bax ratio was also seen in myocytes treated with H2S after H2O2-induced stress. Our findings indicated a therapeutic potential for H2S in preventing myocyte death following ischemia/reperfusion.
Palabras clave
Texto completo:
1
Base de datos:
LILACS
Asunto principal:
Apoptosis
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Mioblastos Cardíacos
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Peróxido de Hidrógeno
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Antioxidantes
Tipo de estudio:
Evaluation_studies
Idioma:
En
Revista:
Braz. j. med. biol. res
/
Rev. bras. pesqui. méd. biol
Asunto de la revista:
BIOLOGIA
/
MEDICINA
Año:
2019
Tipo del documento:
Article