DNA extraction from fixed cytogenetic cell suspensions
Genet. mol. res. (Online)
; 6(3): 500-503, 2007. ilus
Article
en En
| LILACS
| ID: lil-498922
Biblioteca responsable:
BR26.1
ABSTRACT
We developed a procedure for DNA extraction from small volumes of fixed cell suspensions previously prepared for conventional cytogenetic analysis. Good quality DNA was isolated with a fast and simple protocol using DNAzol reagent. This provided suitable DNA for various types of molecular analyses, including polymerase chain reaction, restriction fragment length polymorphism, denaturing high-performance liquid chromatography, and direct sequencing. This technique provides sufficient material for such test, which are important for diagnosis of neoplastic diseases in pediatric patients.
Texto completo:
1
Base de datos:
LILACS
Asunto principal:
ADN
/
Genoma
/
Citogenética
/
Neoplasias
Tipo de estudio:
Guideline
Idioma:
En
Revista:
Genet. mol. res. (Online)
Asunto de la revista:
BIOLOGIA MOLECULAR
/
GENETICA
Año:
2007
Tipo del documento:
Article
/
Project document