Optimization of growth medium for protease production by Haloferax Lucentensis VKMM 007 by response surface methodology
Braz. j. microbiol
; Braz. j. microbiol;42(2): 818-824, Apr.-June 2011. ilus, graf, tab
Article
en En
| LILACS
| ID: lil-590027
Biblioteca responsable:
BR1.1
ABSTRACT
The production of halophilic thermostable protease by Haloferax lucentensis VKMM 007 was optimized using a statistical approach. In accordance with factorial design, soluble starch, gelatin, KCl and MgSO4 were selected among 27 variables tested. Next, a second-order quadratic model was estimated and optimal medium concentrations were determined based on quadratic regression equation generated by model. These were 5.14 g L-1 of KCl, 6.57 g L-1of MgSO4, 9.05 g L-1of gelatin and 5.27 g L-1of soluble starch in high salts media supplemented with 0.5 percent (w/v) of beef extract and peptone, respectively. In these optimal conditions, the obtained protease concentration of 6.80 U mL-1 was in agreement with the predicted protease concentration and was further improved to 7.02 U mL-1 by increasing the concentration of NaCl in the medium to 25 percent (w/v). An overall 4.0-fold increase in protease production was achieved in the optimized medium compared to activity obtained in initial medium.
Texto completo:
1
Base de datos:
LILACS
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Braz J Microbiol
/
Braz. j. microbiol
/
Braz. j. microbiol. (Online)
/
Brazilian journal of microbiology (Impresso)
Asunto de la revista:
MICROBIOLOGIA
Año:
2011
Tipo del documento:
Article