Evaluation of antimicrobial activity of extracts of Tibouchina candolleana (melastomataceae), isolated compounds and semi-synthetic derivatives against endodontic bacteria
Braz. j. microbiol
; 43(2): 793-799, Apr.-June 2012. tab
Article
en En
| LILACS
| ID: lil-644498
Biblioteca responsable:
BR32.1
ABSTRACT
This work describes the phytochemical study of the extracts from aerial parts of Tibouchina candolleana as well as the evaluation of the antimicrobial activity of extracts, isolated compounds, and semi-synthetic derivatives of ursolic acid against endodontic bacteria. HRGC analysis of the n-hexane extract of T. candolleana allowed identification of b-amyrin, a-amyrin, and b-sitosterol as major constituents. The triterpenes ursolic acid and oleanolic acid were isolated from the methylene chloride extract and identified. In addition, the flavonoids luteolin and genistein were isolated from the ethanol extract and identified. The antimicrobial activity was investigated via determination of the minimum inhibitory concentration (MIC) using the broth microdilution method. Amongst the isolated compounds, ursolic acid was the most effective against the selected endodontic bacteria. As for the semi-synthetic ursolic acid derivatives, only the methyl ester derivative potentiated the activity against Bacteroides fragilis.
Palabras clave
Texto completo:
1
Base de datos:
LILACS
Asunto principal:
Triterpenos
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Extractos Vegetales
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Ácidos Oléicos
/
Estructuras de las Plantas
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Genisteína
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Melastomataceae
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Cloruro de Metileno
Tipo de estudio:
Evaluation_studies
Idioma:
En
Revista:
Braz. j. microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2012
Tipo del documento:
Article