Isolation and characterization of canine parvovirus type 2c (CPV-2c) from symptomatic puppies
Braz. j. microbiol
; Braz. j. microbiol;43(3): 1005-1009, July-Sept. 2012. ilus
Article
en En
| LILACS
| ID: lil-656665
Biblioteca responsable:
BR32.1
ABSTRACT
Canine parvovirus type 2 (CPV-2) is a leading cause of diarrhea in puppies in several parts of the world. In this study CPV-2 was detected and recovered from puppies showing clinical disease from Montevideo, Uruguay. Samples were processed and used to infect CRFK and MDCK cells in order to isolate the virus. Out of twelve, two samples were positive for CPV-2. A genomic region of 583 bp was amplified and the molecular characterization was performed by sequencing, phylogenetic analysis and Restriction Fragment Length Polymorphism (RFLP). Two isolated viruses (UY1 and UY2) were CPV-2c-like viruses. The comparison between the cytophatic effect (CPE) of CPV-2 (vaccinal virus) and CPV-2c (isolated virus) on primary canine cells cultures and on CRFK line cells, demonstrated that CPV-2c is less citopathogenic in CRFK than in primary cultures. Our study represents the first report on isolation and characterization of canine parvovirus type 2c (CPV-2c) in cell cultures from South American dogs.
Palabras clave
Texto completo:
1
Base de datos:
LILACS
Asunto principal:
Filogenia
/
Técnicas In Vitro
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Secuencia de Bases
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Genoma Viral
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Parvovirus Canino
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Infecciones por Parvoviridae
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Diarrea
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Braz. j. microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2012
Tipo del documento:
Article