A role for the Cdc7 kinase regulatory subunit Dbf4p in the formation of initiation-competent origins of replication.
Genes Dev
; 13(16): 2159-76, 1999 Aug 15.
Article
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| MEDLINE
| ID: mdl-10465792
ABSTRACT
Using a reconstituted DNA replication assay from yeast, we demonstrate that two kinase complexes are essential for the promotion of replication in vitro. An active Clb/Cdc28 kinase complex, or its vertebrate equivalent, is required in trans to stimulate initiation in G(1)-phase nuclei, whereas the Dbf4/Cdc7 kinase complex must be provided by the template nuclei themselves. The regulatory subunit of Cdc7p, Dbf4p, accumulates during late G(1) phase, becomes chromatin associated prior to Clb/Cdc28 activation, and assumes a punctate pattern of localization that is similar to, and dependent on, the origin recognition complex (ORC). The association of Dbf4p with a detergent-insoluble chromatin fraction in G(1)-phase nuclei requires ORC but not Cdc6p or Clb/Cdc28 kinase activity, and correlates with competence for initiation. We propose a model in which Dbf4p targets Cdc7p to the prereplication complex prior to the G(1)/S transition, by a pathway parallel to, but independent of, the Cdc6p-dependent recruitment of MCMs.
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1
Base de datos:
MEDLINE
Asunto principal:
ADN de Hongos
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Proteínas Fúngicas
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Proteínas Serina-Treonina Quinasas
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Origen de Réplica
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Proteínas de Ciclo Celular
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Proteínas de Saccharomyces cerevisiae
Idioma:
En
Revista:
Genes Dev
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
1999
Tipo del documento:
Article