Activated T lymphocytes support osteoclast formation in vitro.
Biochem Biophys Res Commun
; 265(1): 144-50, 1999 Nov.
Article
en En
| MEDLINE
| ID: mdl-10548505
ABSTRACT
Osteoblastic stromal cells are capable of supporting osteoclast formation from hematopoietic precursors in the presence of osteotropic factors such as 1alpha,25(OH)(2)D(3), PTH, and IL-11. Osteoblastic stromal cells produce receptor activator of NF-kappaB ligand (RANKL), a type II membrane protein of the TNF ligand family, in response to these agents. Activated T lymphocytes also produce RANKL; however, the ability of this cell type to support osteoclast formation in vitro is unknown. Human PBMC-derived T cells, extracted using alphaCD3-coated magnetic beads, were cocultured with adherent murine spleen cells in the presence of Con A and a panel of cytokines. In the presence of Con A, bona fide osteoclasts were formed in vitro with activated T cells IL-1alpha and TGFbeta further enhanced osteoclast numbers. PBMC-derived lymphocytes showed an increase in the mRNA expression of RANKL within 24 h of treatment with the same agents that were used to induce osteoclast formation. In synovial tissue sections with lymphoid infiltrates from RA patients, the expression of RANKL was demonstrated in CD3(+) T cells. The ability of activated T lymphocytes to support osteoclast formation may provide a mechanism for the potentiation of osteoclast formation and bone resorption in disease states such as rheumatoid arthritis.
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Base de datos:
MEDLINE
Asunto principal:
Osteoclastos
/
Células Madre Hematopoyéticas
/
Glicoproteínas de Membrana
/
Activación de Linfocitos
/
Linfocitos T
/
Proteínas Portadoras
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
1999
Tipo del documento:
Article