Purification, crystallization and x-ray analysis of crystals of pectate lyase A from Exwinia chrysanthemi.

Doan, C N; Caughron, M K; Myers, J C; Breakfield, N W; Oliver, R L; Yoder, M D

Doan, C N; Caughron, M K; Myers, J C; Breakfield, N W; Oliver, R L; Yoder, M D.

Afiliación

Doan CN; School of Biological Sciences, University of Missouri-Kansas City, Kansas City, MO 64110--2499, USA.

Acta Crystallogr D Biol Crystallogr ; 56(Pt 3): 351-3, 2000 Mar.

Article en En | MEDLINE | ID: mdl-10713524

ABSTRACT

ABSTRACT

Pectate lyase A is secreted by Erwinia chrysanthemi and is a virulence factor for soft rot diseases in plants. Crystals of pectate lyase A were obtained by vapor-diffusion techniques in the presence of polyethylene glycol. The crystals belong to the monoclinic space group P2(1), with unit-cell parameters a = 48.96, b = 148.86, c = 78.61 A, beta = 97.32 degrees. The crystals contain two protein molecules of 38 kDa per asymmetric unit and diffract to 2.4 A using Cu Kalpha radiation.

Asunto(s)

Proteínas Bacterianas/química; Dickeya chrysanthemi/enzimología; Polisacárido Liasas/química; Proteínas Bacterianas/aislamiento & purificación; Cristalografía por Rayos X; Modelos Moleculares; Polisacárido Liasas/aislamiento & purificación; Conformación Proteica

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Base de datos: MEDLINE Asunto principal: Polisacárido Liasas / Proteínas Bacterianas / Dickeya chrysanthemi Tipo de estudio: Prognostic_studies Idioma: En Revista: Acta Crystallogr D Biol Crystallogr Año: 2000 Tipo del documento: Article

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