Cytogenetic analysis from DNA by comparative genomic hybridization.
Ann Genet
; 43(3-4): 147-54, 2000.
Article
en En
| MEDLINE
| ID: mdl-11164197
ABSTRACT
Comparative genomic hybridization (CGH) is a modified in situ hybridization technique which allows detection and mapping of DNA sequence copy differences between two genomes in a single experiment. In CGH analysis, two differentially labelled genomic DNA (study and reference) are co-hybridized to normal metaphase spreads. Chromosomal locations of copy number changes in the DNA segments of the study genome are revealed by a variable fluorescence intensity ratio along each target chromosome. Since its development, CGH has been applied mostly as a research tool in the field of cancer cytogenetics to identify genetic changes in many previously unknown regions. CGH may also have a role in clinical cytogenetics for detection and identification of unbalanced chromosomal abnormalities.
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Base de datos:
MEDLINE
Asunto principal:
ADN
/
Aberraciones Cromosómicas
/
Mapeo Cromosómico
/
Hibridación in Situ
/
Citogenética
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Ann Genet
Año:
2000
Tipo del documento:
Article