Protein phosphatase 2A: identification in Oryza sativa of the gene encoding the regulatory A subunit.
Plant Mol Biol
; 45(1): 107-12, 2001 Jan.
Article
en En
| MEDLINE
| ID: mdl-11247601
ABSTRACT
A 2225 bp cDNA, designated RPA1, was isolated from an Oryza sativa cDNA library. Analysis revealed a 1761 bp coding sequence with 15 non-identical repeat units. The ORF encoded the A regulatory subunit of protein phosphatase 2A (PP2A-A) as ascertained by complementation of the yeast tpd3 mutant defective in this gene. The corresponding genomic DNA from a rice genome BAC library revealed that the gene contains eleven introns. The rice genome contains only a single copy of this gene as judged by Southern blot analysis. The PP2A protein is highly conserved in nature; the rice protein shows 88% amino acid identity with its counterparts in Arabidopsis or Nicotiana tabacum.
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Base de datos:
MEDLINE
Asunto principal:
Proteínas de Plantas
/
Oryza
/
Genes de Plantas
/
Fosfoproteínas Fosfatasas
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Plant Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BOTANICA
Año:
2001
Tipo del documento:
Article