Synergistic stimulation, by tumor necrosis factor-alpha and interferon-gamma, of fractalkine expression in human astrocytes.
Neurosci Lett
; 303(2): 132-6, 2001 May 04.
Article
en En
| MEDLINE
| ID: mdl-11311510
ABSTRACT
Fractalkine is a CX3C chemonkine that appears to be a neuron-to-microglia signal molecule in the central nervous system. We studied the expression of fractalkine in normal human astrocytes in culture, by using semi-quantitative reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay. We found that tumor-necrosis factor alpha (TNF-alpha) and interferon-gamma (IFN-gamma) synergistically enhance the expression of fractalkine. The expression of both fractalkine mRNA and protein was increased in time- and concentration-dependent manners in the cells co-stimulated with TNF-alpha and IFN-gamma. Cycloheximide, an inhibitor of protein synthesis, and dexamethasone had no effect on the synergy of the stimulation of fractalkine expression. We conclude that normal human astrocytes produce fractalkine by co-stimulation with pro-inflammatory cytokines and it may serve as a potential signal for immune and inflammatory responses in the central nervous system.
Buscar en Google
Base de datos:
MEDLINE
Asunto principal:
Comunicación Celular
/
Astrocitos
/
Interferón gamma
/
Factor de Necrosis Tumoral alfa
/
Quimiocinas CX3C
/
Interacciones Farmacológicas
/
Encefalitis
/
Proteínas de la Membrana
Idioma:
En
Revista:
Neurosci Lett
Año:
2001
Tipo del documento:
Article