Negative regulation of YY1 transcription factor on the dynamin I gene promoter.
Biochem Biophys Res Commun
; 283(2): 340-3, 2001 May 04.
Article
en En
| MEDLINE
| ID: mdl-11327704
ABSTRACT
Dynamin I is highly expressed in brain and plays a critical role in clathrin-mediated endocytosis and synaptic vesicle recycling. To elucidate the molecular mechanism by which expression of dynamin I is tissue-specifically regulated, we previously cloned and characterized the promoter of the mouse dynamin I gene and suggested that there is a negative regulatory element in this promoter region. In the present study, we showed that YY1 binds to this negative regulatory element located at -111 to -107 by using the EMSA and supershift analyses. Cotransfection experiment using an YY1 expression vector revealed that YY1 exerts a repressive role on the dynamin I gene promoter activity. These results demonstrate that transcription factor YY1 negatively regulates dynamin I expression via binding to the negative regulatory element.
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Base de datos:
MEDLINE
Asunto principal:
Factores de Transcripción
/
Regiones Promotoras Genéticas
/
Proteínas de Unión al ADN
/
GTP Fosfohidrolasas
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2001
Tipo del documento:
Article