Definition of an optimal cytotoxic T lymphocyte epitope in the latently expressed Kaposi's sarcoma-associated herpesvirus kaposin protein.
J Infect Dis
; 184(2): 119-26, 2001 Jul 15.
Article
en En
| MEDLINE
| ID: mdl-11424007
ABSTRACT
Cytotoxic T lymphocytes (CTL) recognize and kill virus-infected cells and contribute to immunologic control of viral replication. For many herpesviruses (e.g., Epstein-Barr and cytomegalovirus), virus-specific CTL responses can be readily detected in infected persons, but CTL responses against Kaposi's sarcoma-associated herpesvirus (KSHV) appear to be weak and remain poorly characterized. Using a human leukocyte antigen (HLA) binding motif-based epitope prediction algorithm, we identified 37 HLA-A*0201 binding peptides from 8 KSHV open-reading frames (ORFs). After in vitro stimulation of peripheral blood mononuclear cells from KSHV-infected persons, CTL responses against 1 peptide in the KSHV kaposin protein (ORF K12) were detected in 2 HLA-A*0201-positive subjects. The optimal CTL epitope was identified by HLA restriction analysis and peptide titration assays. These data describe a latent phase viral gene product targeted by CTL that may be relevant for KSHV immunopathogenesis.
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Base de datos:
MEDLINE
Asunto principal:
Proteínas Virales
/
Linfocitos T Citotóxicos
/
Antígenos HLA-A
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Síndrome de Inmunodeficiencia Adquirida
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Herpesvirus Humano 8
/
Epítopos
Tipo de estudio:
Risk_factors_studies
Idioma:
En
Revista:
J Infect Dis
Año:
2001
Tipo del documento:
Article