Preconditioning decreases ischemia/reperfusion-induced peroxynitrite formation.
Biochem Biophys Res Commun
; 285(5): 1217-9, 2001 Aug 03.
Article
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| MEDLINE
| ID: mdl-11478785
ABSTRACT
The role for peroxynitrite (ONOO(-)) in the mechanism of preconditioning is not known. Therefore, we studied effects of preconditioning and subsequent ischemia/reperfusion on myocardial ONOO(-) formation in isolated rat hearts. Hearts were subjected to a preconditioning protocol (three intermittent periods of global ischemia/reperfusion of 5 min duration each) followed by a test ischemia/reperfusion (30 min global ischemia and 15 min reperfusion). When compared to nonpreconditioned controls, preceding preconditioning improved postischemic cardiac performance and significantly decreased test ischemia/reperfusion-induced formation of free nitrotyrosine measured in the perfusate as a marker for cardiac endogenous ONOO(-) formation. During preconditioning, however, the first period of ischemia/reperfusion increased nitrotyrosine formation, which was attenuated after the third period of ischemia/reperfusion. We conclude that classic preconditioning inhibits ischemia/reperfusion-induced cardiac formation of ONOO(-) and that subsequent periods of ischemia/reperfusion result in a gradual attenuation of ischemia/reperfusion-induced ONOO(-) generation. This mechanism might be involved in ischemic adaptation of the heart.
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Base de datos:
MEDLINE
Asunto principal:
Tirosina
/
Reperfusión Miocárdica
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Isquemia Miocárdica
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Precondicionamiento Isquémico Miocárdico
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Miocardio
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Nitratos
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2001
Tipo del documento:
Article