Hypermethylation of CpG islands in the mouse asparagine synthetase gene: relationship to asparaginase sensitivity in lymphoma cells. Partial methylation in normal cells.
Br J Cancer
; 85(6): 930-5, 2001 Sep 14.
Article
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| MEDLINE
| ID: mdl-11556848
ABSTRACT
We have sequenced the promoter region of the murine asparagine synthetase gene and examined its methylation profile in the CpG islands of L-asparaginase-sensitive 6C3HED cells (asparagine auxotrophs) and resistant variants (prototrophs). In the former, complete methylation of the CpG island is correlated with failure of expression of mRNA cells of the latter possess both methylated and unmethylated alleles, as do cells of the intrinsically asparagine-independent lines L1210 and EL4. A similar phenomenon was seen in normal splenic cells of adult mice. This was age related no methylation was found in weanlings, but up to 45% of gene copies in animals 18 weeks or older were methylated. It was also tissue related, with methylation occurring rarely in liver cells. The relationship of these changes to oncogenesis is considered.
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1
Base de datos:
MEDLINE
Asunto principal:
Asparaginasa
/
Aspartatoamoníaco Ligasa
/
Islas de CpG
/
Linfoma
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Br J Cancer
Año:
2001
Tipo del documento:
Article