Meconium aspiration produces airway hyperresponsiveness and eosinophilic inflammation in a murine model.
Am J Physiol Lung Cell Mol Physiol
; 283(4): L785-90, 2002 Oct.
Article
en En
| MEDLINE
| ID: mdl-12225955
ABSTRACT
Meconium aspiration syndrome is a cause of significant morbidity and mortality in the perinatal period and has been implicated in the pathogenesis of airway dysfunction. In this study, we developed a murine model to evaluate the effects of meconium aspiration on airway physiology and lung cellular responses. Under light anesthesia, BALB/c mice received a single intratracheal instillation of meconium or physiological saline. Respiratory mechanics were measured in unrestrained animals and expressed as percent increase in enhanced pause to increasing concentrations of methacholine (MCh). Furthermore, we assessed the changes in cells and cytokines into the bronchoalveolar lavage fluid (BALF). We found meconium aspiration produced increased airway responsiveness to MCh at 7 days. These functional changes were associated with lymphocytic/eosinophilic inflammation, goblet cell metaplasia, and increased concentrations of IL-5 and IL-13 in the BALF. Our findings suggest meconium aspiration leads to alterations of airway function, lung eosinophilia, goblet cell metaplasia, and cytokine imbalance, thus providing the first evidence of meconium-induced airway dysfunction in a mouse model.
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Base de datos:
MEDLINE
Asunto principal:
Síndrome de Aspiración de Meconio
/
Hiperreactividad Bronquial
/
Eosinófilos
Tipo de estudio:
Etiology_studies
/
Prognostic_studies
Idioma:
En
Revista:
Am J Physiol Lung Cell Mol Physiol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
FISIOLOGIA
Año:
2002
Tipo del documento:
Article