[Effect of MNNG on ornithine decarboxylase activity in cells from adult Schistosoma japonicum].

OBJECTIVE: To observe the change of ornithine decarboxylase(ODC) activity in cells from adult Schistosoma japonicum after the cells were treated with N-Methyl-N-Nitro-N-Nitrosoguanidine (MNNG). METHODS: The cells were treated with MNNG at a concentration of 3 micrograms/ml for 48 hours after the cells being incubated for one week. The cells were then cultured with RPMI-1640 containing 10% calf serum. ODC activity was detected with spectrophotography. RESULTS: ODC activity rose significantly in two to three weeks after the cells were treated with MNNG. CONCLUSION: There was ODC activity in cells from adult S. japonicum and MNNG has an effect to reinforce ODC activity in the cells.