A simple pen-spotting method for arraying biomolecules on solid substrates.
Biosens Bioelectron
; 18(12): 1455-9, 2003 Oct 15.
Article
en En
| MEDLINE
| ID: mdl-12941560
ABSTRACT
We describe a simple, relatively inexpensive method for depositing biomolecules on a solid substrate using Rapidograph drafting pens. The pens can be used without modification to accurately deposit spots between approximately 100 and 600 microm in diameter. When mounted on a suitable microtranslation stage, the pens can be used to easily deposit tens of spots aligned with underlying substrate features such as microfabricated sensors. The pens are particularly convenient because pre-mixed solutions can be stored in the pens for multiple uses. We demonstrate the use of this approach to deposit DNA probes on a microsensor array.
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Base de datos:
MEDLINE
Asunto principal:
Biopolímeros
/
ADN
/
Sondas Moleculares
/
Materiales Biocompatibles Revestidos
/
Análisis de Secuencia por Matrices de Oligonucleótidos
/
Micromanipulación
Tipo de estudio:
Evaluation_studies
Idioma:
En
Revista:
Biosens Bioelectron
Asunto de la revista:
BIOTECNOLOGIA
Año:
2003
Tipo del documento:
Article