Induction of plasminogen activator inhibitor-2 is associated with suppression of invasive activity in TPA-mediated differentiation of human prostate cancer cells.
Biochem Biophys Res Commun
; 309(2): 267-71, 2003 Sep 19.
Article
en En
| MEDLINE
| ID: mdl-12951044
ABSTRACT
We previously reported that 12-O-tetra-decanoylphorbol-13-acetate (TPA) induces microglia-like differentiation and decreases malignancy in human prostate cancer TSU-Pr1 cells. To investigate the mechanism underlying differentiation and decrease of malignancy in TSU-Pr1 cells treated with TPA, we attempted to identify genes expressed differentially during the differentiation using differential display. We successfully detected plasminogen activator inhibitor type-2 (PAI-2) as one gene up-regulated by TPA treatment. The change in expression of PAI-2 by TPA was blocked by treatment with protein kinase C or mitogen-activated protein kinase inhibitors. We also found that secretion of PAI-2 protein was increased by TPA treatment. Moreover, we demonstrated that suppression of invasive activity of TSU-Pr1 cells by TPA treatment was blocked by co-treatment with anti-PAI-2 antibody. These results suggest that induction of PAI-2 is associated with suppression of invasive activity in TSU-Pr1 cells treated with TPA.
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Base de datos:
MEDLINE
Asunto principal:
Neoplasias de la Próstata
/
Acetato de Tetradecanoilforbol
/
Regulación Neoplásica de la Expresión Génica
/
Inhibidor 2 de Activador Plasminogénico
Tipo de estudio:
Risk_factors_studies
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2003
Tipo del documento:
Article