Isolation of tocopherol-binding proteins from the cytosol of smooth muscle A7r5 cells.
Eur J Biochem
; 209(1): 37-42, 1992 Oct 01.
Article
en En
| MEDLINE
| ID: mdl-1396710
Cultured smooth muscle A7r5 cells were able to take up alpha-tocopherol (32 +/- 1.2 nmol/mg protein) the largest part of which (60%) was present in the cytosolic fraction. Using a tocopherol-based affinity chromatography and alpha-, beta-, gamma-, and delta-tocopherols as eluants, three polypeptides of molecular masses 81, 58 and 31 kDa were eluted. This preparation had alpha-[3H]tocopherol binding capability. The 58-kDa polypeptide could also be eluted by chromanol and the 81-kDa polypeptide could be eluted also by phytol. The 81-kDa polypeptide had the unique P-E-E-D-Q-X-Q-Y N-terminal sequence.
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Base de datos:
MEDLINE
Asunto principal:
Proteínas Portadoras
/
Músculo Liso Vascular
Idioma:
En
Revista:
Eur J Biochem
Año:
1992
Tipo del documento:
Article