Use of reporter cell lines for detection of endocrine-disrupter activity.
Anal Bioanal Chem
; 378(3): 655-63, 2004 Feb.
Article
en En
| MEDLINE
| ID: mdl-14513195
ABSTRACT
We have studied stable transformed human mammary cell lines with highly inducible steroid receptor-mediated luciferase reporter gene expression. Cells responding specifically to glucocorticoids, progestagens, androgens, or estrogens are described and characterized. The use of this high-throughput, cell-based assay for analysis of steroid (ant)agonists is reported. Systematic characterization of endocrine-disrupting activity on human receptors and in a human-cell system is interpreted for a selection of xenobiotics. We show that the phytoestrogens apigenin and genistin have progestagenic and androgenic activity, respectively. Finally, application of cell-based assays to the analysis of environmental samples is discussed.
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Base de datos:
MEDLINE
Asunto principal:
Genes Reporteros
/
Glándulas Endocrinas
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Anal Bioanal Chem
Año:
2004
Tipo del documento:
Article