A catalog of human cDNA expression clones and its application to structural genomics.
Genome Biol
; 5(9): R71, 2004.
Article
en En
| MEDLINE
| ID: mdl-15345055
ABSTRACT
We describe here a systematic approach to the identification of human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli. A cDNA expression library of 10,825 clones was screened by small-scale expression and purification and 2,746 clones were identified. Sequence and protein-expression data were entered into a public database. A set of 163 clones was selected for structural analysis and 17 proteins were prepared for crystallization, leading to three new structures.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Biblioteca de Genes
/
Clonación Molecular
/
ADN Complementario
/
Genómica
Idioma:
En
Revista:
Genome Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
GENETICA
Año:
2004
Tipo del documento:
Article