Immunogenicity of murein-associated proteins from temperature-stressed Streptococcus suis cultures.
J Vet Med B Infect Dis Vet Public Health
; 51(6): 272-7, 2004 Aug.
Article
en En
| MEDLINE
| ID: mdl-15458489
ABSTRACT
We compared immunogenicity in pigs of whole cell lysate proteins (WCP) with murein-associated proteins (MAP) obtained from a virulent serotype 2 strain of Streptococcus (S.) suis grown at 32 or 42 degrees C. Protein fractions were tested for their ability to induce antibodies in 3-week-old piglets by enzyme-linked immunosorbent assay and Western blot analysis. We found a significant increase in the antibody levels in all sera irrespective of the preparation used for immunization. However, alpha-WCP sera showed higher reactivities than alpha-MAP sera, and piglets immunized with 32 degrees C preparations (alpha-32 sera) showed higher responses than those immunized with 42 degrees C preparations (alpha-42 sera). Western blot analysis revealed that alpha-WCP sera in part reacted with different proteins when compared with alpha-MAP sera. Furthermore, some proteins were only detected by alpha-32 but not by alpha-42 sera. In conclusion, the results demonstrate the immunogenicity of cell wall MAP of S. suis, and highlight the importance of considering growth conditions in the preparation of subunit vaccines.
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Base de datos:
MEDLINE
Asunto principal:
Infecciones Estreptocócicas
/
Enfermedades de los Porcinos
/
Peptidoglicano
/
Streptococcus suis
Tipo de estudio:
Risk_factors_studies
Idioma:
En
Revista:
J Vet Med B Infect Dis Vet Public Health
Asunto de la revista:
MEDICINA VETERINARIA
/
SAUDE PUBLICA
Año:
2004
Tipo del documento:
Article