Antiangiogenic properties of fibstatin, an extracellular FGF-2-binding polypeptide.
Cancer Res
; 64(20): 7507-12, 2004 Oct 15.
Article
en En
| MEDLINE
| ID: mdl-15492277
ABSTRACT
By using the two-hybrid system with basic fibroblast growth factor (FGF-2) as bait, we isolated and characterized fibstatin, an endogenous M(r) 29,000 human basement membrane-derived inhibitor of angiogenesis and tumor growth. Fibstatin, a fragment containing the type III domains 12-14 of fibronectin, was produced as a recombinant protein and was shown to inhibit the proliferation, migration, and differentiation of endothelial cells in vitro. Antiangiogenic activity of fibstatin was confirmed in a Matrigel angiogenesis assay in vivo, and electrotransfer of the fibstatin gene into muscle tissue resulted in reduced B16F10 tumor growth. Taken together, these results suggest that fibstatin could act as a powerful molecule for antiangiogenic therapy.
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Base de datos:
MEDLINE
Asunto principal:
Proteínas Portadoras
/
Factor 2 de Crecimiento de Fibroblastos
/
Proteínas de la Membrana
Idioma:
En
Revista:
Cancer Res
Año:
2004
Tipo del documento:
Article