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Cartilage-derived morphogenetic protein-1 promotes the differentiation of mesenchymal stem cells into chondrocytes.
Bai, Xiaowen; Xiao, Zhifeng; Pan, Yuqiong; Hu, Jiang; Pohl, Jens; Wen, Jinhua; Li, Lingsong.
Afiliación
  • Bai X; Peking University Stem Cell Research Center, Peking University Health Science Center, 38 Xue Yuan Road, Beijing 100083, China.
Biochem Biophys Res Commun ; 325(2): 453-60, 2004 Dec 10.
Article en En | MEDLINE | ID: mdl-15530414
Mesenchymal stem cells (MSCs) are able to differentiate into many types of cells including chondrocytes. Transforming growth factor beta1 (TGF-beta1) is very important in the regulation of chondrogenesis. Since cartilage-derived morphogenetic protein-1 (CDMP-1) belongs to the TGF-beta superfamily, we tested whether CDMP-1 plays any role in the regulation of the differentiation of MSCs into chondrocytes using a high density pellet culture system. Based on the histological staining of glycosaminoglycan using toluidine blue dye-binding method we found that CDMP-1 could initiate chondrogenic differentiation of MSCs as did TGF-beta1. However, CDMP-1 was less stimulatory than TGF-beta1. The combination of CDMP-1 and TGF-beta1 synergically induced chondrogenesis of MSCs. This synergic chondrogenic effect of CDMP-1 together with TGF-beta1 was further confirmed by quantification of GAG using dimethylmethylene blue dye-binding assay and immunohistochemical analysis of the expression of cartilage-specific protein collagen II. This study may provide an improved induction approach using MSCs for repairing damaged cartilage.
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Base de datos: MEDLINE Asunto principal: Diferenciación Celular / Proteínas Morfogenéticas Óseas / Condrocitos / Células Madre Mesenquimatosas Idioma: En Revista: Biochem Biophys Res Commun Año: 2004 Tipo del documento: Article
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Base de datos: MEDLINE Asunto principal: Diferenciación Celular / Proteínas Morfogenéticas Óseas / Condrocitos / Células Madre Mesenquimatosas Idioma: En Revista: Biochem Biophys Res Commun Año: 2004 Tipo del documento: Article