Method for detection of respiratory viruses in the sputa of patients with cystic fibrosis.
Eur J Clin Microbiol Infect Dis
; 24(1): 54-7, 2005 Jan.
Article
en En
| MEDLINE
| ID: mdl-15616837
ABSTRACT
Since the role of respiratory viruses in lung exacerbations of patients with cystic fibrosis has been hampered by the difficulty of detecting viruses in viscous sputum specimens, a multiplex reverse transcriptase PCR (RT-PCR) assay combined with colorimetric amplicon detection was tested for the identification of seven common respiratory viruses in the sputa of cystic fibrosis patients. Of 52 sputa from 38 patients, 12 (23%) samples from 12 patients were positive for a respiratory virus (4 for influenza B, 3 for parainfluenza 1, 3 for influenza A and 2 for respiratory syncytial virus). These results suggest that the RT-PCR method carried out on sputum may provide a convenient means of investigating the role of virus infection in lung exacerbations of cystic fibrosis patients.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Virus Sincitiales Respiratorios
/
Esputo
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Infecciones por Virus Sincitial Respiratorio
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Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
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Fibrosis Quística
Tipo de estudio:
Diagnostic_studies
/
Incidence_studies
/
Observational_studies
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Prognostic_studies
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Risk_factors_studies
Idioma:
En
Revista:
Eur J Clin Microbiol Infect Dis
Asunto de la revista:
DOENCAS TRANSMISSIVEIS
/
MICROBIOLOGIA
Año:
2005
Tipo del documento:
Article