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Spectral signal-to-noise ratio and resolution assessment of 3D reconstructions.
Unser, M; Sorzano, C O S; Thévenaz, P; Jonic, S; El-Bez, C; De Carlo, S; Conway, J F; Trus, B L.
Afiliación
  • Unser M; Biomedical Imaging Group, Swiss Federal Institute of Technology Lausanne, CH-1015 Lausanne VD, Switzerland.
J Struct Biol ; 149(3): 243-55, 2005 Mar.
Article en En | MEDLINE | ID: mdl-15721578
ABSTRACT
Measuring the quality of three-dimensional (3D) reconstructed biological macromolecules by transmission electron microscopy is still an open problem. In this article, we extend the applicability of the spectral signal-to-noise ratio (SSNR) to the evaluation of 3D volumes reconstructed with any reconstruction algorithm. The basis of the method is to measure the consistency between the data and a corresponding set of reprojections computed for the reconstructed 3D map. The idiosyncrasies of the reconstruction algorithm are taken explicitly into account by performing a noise-only reconstruction. This results in the definition of a 3D SSNR which provides an objective indicator of the quality of the 3D reconstruction. Furthermore, the information to build the SSNR can be used to produce a volumetric SSNR (VSSNR). Our method overcomes the need to divide the data set in two. It also provides a direct measure of the performance of the reconstruction algorithm itself; this latter information is typically not available with the standard resolution methods which are primarily focused on reproducibility alone.
Asunto(s)

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Procesamiento de Imagen Asistido por Computador / Microscopía Electrónica / Imagenología Tridimensional Idioma: En Revista: J Struct Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2005 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Procesamiento de Imagen Asistido por Computador / Microscopía Electrónica / Imagenología Tridimensional Idioma: En Revista: J Struct Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2005 Tipo del documento: Article