Evaluation of lymph node and bone marrow cytology in the diagnosis of canine leishmaniasis (Leishmania infantum) in symptomatic and asymptomatic dogs.
Am J Trop Med Hyg
; 73(1): 82-6, 2005 Jul.
Article
en En
| MEDLINE
| ID: mdl-16014839
ABSTRACT
The sensitivity and specificity of lymph node and bone marrow smear microscopy for the diagnosis of Leishmania infantum-infected dogs was evaluated in 79 dogs with leishmaniasis (Group A), 52 asymptomatically infected dogs (Group B), and 44 healthy noninfected dogs (Group C). Light microscopy examination included 10 to 1,000 oil immersion fields, and the density of Leishmania amastigotes was scored by a 0 to +6 scale. Using polymerase chain reaction as the gold standard, the specificity of lymph node and bone marrow cytology was 100%, whereas sensitivity ranged from 7.8% to 92.6%, being significantly higher in Group A compared with Group B. The amastigote scores were also significantly higher in Group A compared with Group B. These results indicate that lymph node and bone marrow cytology is a highly sensitive and specific method for the diagnosis of canine patent leishmaniasis, whereas its sensitivity is relatively low in asymptomatic infections.
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Base de datos:
MEDLINE
Asunto principal:
Médula Ósea
/
Leishmania infantum
/
Enfermedades de los Perros
/
Leishmaniasis Visceral
/
Ganglios Linfáticos
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Am J Trop Med Hyg
Año:
2005
Tipo del documento:
Article