[Inhibition of hepatitis B virus replication and expression by RNA interference in vivo].
Zhonghua Gan Zang Bing Za Zhi
; 14(1): 15-8, 2006 Jan.
Article
en Zh
| MEDLINE
| ID: mdl-16420758
ABSTRACT
OBJECTIVE:
To evaluate the inhibitory effect of small interfering RNA (siRNA) targeting HBV C gene region on hepatitis B virus (HBV) in vivo.METHODS:
An animal model of HBV infection was developed hydrodynamically, and pcDNA3.1-HBV and siRNA were together injected into the tail vein of the BALB/c mice. HBsAg was analyzed by time-resolved immunofluorometric assay, HBV DNA was analyzed by fluorogenic quantitative PCR (FQ-PCR), HBV C-mRNA was detected by semi-quantitative RT-PCR, and viral specific proteins (HBsAg and HBcAg) in the mice livers were assayed using immunohistochemical staining.RESULTS:
In the mice, the siRNA effectively inhibited HBV replication and expression compared with the controls. The inhibitive effect of siRNA on HBV lasted at least 3 days.CONCLUSION:
These results demonstrate that RNAi can substantially inhibit HBV replication and expression in vivo.
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Base de datos:
MEDLINE
Asunto principal:
Replicación Viral
/
Virus de la Hepatitis B
/
ARN Interferente Pequeño
Idioma:
Zh
Revista:
Zhonghua Gan Zang Bing Za Zhi
Asunto de la revista:
GASTROENTEROLOGIA
Año:
2006
Tipo del documento:
Article