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[Inhibition of hepatitis B virus replication and expression by RNA interference in vivo].
Ying, Ruo-su; Fan, Xue-gong; Zhu, Cai; Li, Ning; Zhang, Bao-xin.
Afiliación
  • Ying RS; Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha 410008, China.
Zhonghua Gan Zang Bing Za Zhi ; 14(1): 15-8, 2006 Jan.
Article en Zh | MEDLINE | ID: mdl-16420758
ABSTRACT

OBJECTIVE:

To evaluate the inhibitory effect of small interfering RNA (siRNA) targeting HBV C gene region on hepatitis B virus (HBV) in vivo.

METHODS:

An animal model of HBV infection was developed hydrodynamically, and pcDNA3.1-HBV and siRNA were together injected into the tail vein of the BALB/c mice. HBsAg was analyzed by time-resolved immunofluorometric assay, HBV DNA was analyzed by fluorogenic quantitative PCR (FQ-PCR), HBV C-mRNA was detected by semi-quantitative RT-PCR, and viral specific proteins (HBsAg and HBcAg) in the mice livers were assayed using immunohistochemical staining.

RESULTS:

In the mice, the siRNA effectively inhibited HBV replication and expression compared with the controls. The inhibitive effect of siRNA on HBV lasted at least 3 days.

CONCLUSION:

These results demonstrate that RNAi can substantially inhibit HBV replication and expression in vivo.
Asunto(s)
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Base de datos: MEDLINE Asunto principal: Replicación Viral / Virus de la Hepatitis B / ARN Interferente Pequeño Idioma: Zh Revista: Zhonghua Gan Zang Bing Za Zhi Asunto de la revista: GASTROENTEROLOGIA Año: 2006 Tipo del documento: Article
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Base de datos: MEDLINE Asunto principal: Replicación Viral / Virus de la Hepatitis B / ARN Interferente Pequeño Idioma: Zh Revista: Zhonghua Gan Zang Bing Za Zhi Asunto de la revista: GASTROENTEROLOGIA Año: 2006 Tipo del documento: Article