9-Octadecynoic acid: a novel DNA binding agent.
Chem Res Toxicol
; 4(2): 195-8, 1991.
Article
en En
| MEDLINE
| ID: mdl-1664257
ABSTRACT
A novel bioassay was developed to permit the identification of cytotoxic natural principles that bind to DNA. A hexane extract of Schoepfia californica cytotoxic to cultured KB cells displayed much less cytotoxic potential when the culture medium contained exogenously added calf thymus DNA. Fractionation of the extract afforded a purified principle shown to be 9-octadecynoic acid, an 18-carbon, unbranched acetylenic fatty acid. 9-Octadecynoic acid had an apparent DNA dissociation constant of 1.8 mM; it inhibited topoisomerase I mediated DNA filter binding but did not inhibit the DNA topoisomerase I mediated relaxation of a supercoiled plasmid DNA. The fatty acid was weakly inhibitory to DNA polymerase alpha. 9-Octadecynoic acid possesses none of the structural characteristics of known DNA binding molecules and may bind to DNA by some novel mechanism.
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Base de datos:
MEDLINE
Asunto principal:
ADN
/
Ácidos Grasos Insaturados
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Chem Res Toxicol
Asunto de la revista:
TOXICOLOGIA
Año:
1991
Tipo del documento:
Article