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Interactions between CD3 and Thy1 T cell activation pathways: blockade of CD3-mediated T lymphocyte activation induced by immobilized anti-Thy1 antibodies.
Bellio, M; Leal, L M; Scharfstein, J; Dos Reis, G A.
Afiliación
  • Bellio M; Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Brazil.
Cell Immunol ; 135(2): 534-40, 1991 Jul.
Article en En | MEDLINE | ID: mdl-1674673
ABSTRACT
Resting murine T cell activation induced by either CD3 complexes or Thy1 molecules was investigated in vitro, using surface-bound anti-CD3 mAb as the stimulus. One mitogenic anti-Thy 1 mAb (G7) lost mitogenicity when presented to T cells immobilized on a plastic surface, even in the presence of phorbol ester. Moreover, T cell activation induced by immobilized anti-CD3 was potently blocked by coimmobilized anti-Thy 1 mAb. Nonmitogenic anti-Thy 1 mAb also blocked CD3-induced activation when coimmobilized with anti-CD3. Control experiments showed that anti-Thy 1 specifically blocked T cell activation, even in the presence of measurable and functional concentrations of plastic-bound anti-CD3. Coimmobilized anti-Thy 1 potently blocked IL2 secretion stimulated by anti-CD3. Addition of exogenous rIL2 completely prevented anti-Thy 1-mediated blockade. On the other hand, while completely blocking T cell proliferation, immobilized anti-Thy 1 only partially blocked secretion of IL3-like activity by the T cells. One IgM anti-Thy 1 mAb (2A3) induced secretion of IL3-like activity by T cells when immobilized in the absence of bound anti-CD3. These results indicate that extensive aggregation of Thy 1 molecules delivers a potent negative signal which antagonizes CD3-mediated T cell activation and growth.
Asunto(s)
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Base de datos: MEDLINE Asunto principal: Activación de Linfocitos / Receptores de Antígenos de Linfocitos T / Linfocitos T / Antígenos de Diferenciación de Linfocitos T / Antígenos CD / Anticuerpos Monoclonales / Antígenos de Superficie Idioma: En Revista: Cell Immunol Año: 1991 Tipo del documento: Article
Buscar en Google
Base de datos: MEDLINE Asunto principal: Activación de Linfocitos / Receptores de Antígenos de Linfocitos T / Linfocitos T / Antígenos de Diferenciación de Linfocitos T / Antígenos CD / Anticuerpos Monoclonales / Antígenos de Superficie Idioma: En Revista: Cell Immunol Año: 1991 Tipo del documento: Article