[Expression of anti-HBc single-chain variable fragment mediated by recombinant replication defective adenovirus in vitro].
Zhonghua Gan Zang Bing Za Zhi
; 14(8): 587-9, 2006 Aug.
Article
en Zh
| MEDLINE
| ID: mdl-16938170
OBJECTIVES: To define the expression of single-chain variable fragment (ScFv) against hepatitis B virus core protein (HBc) mediated by recombinant replication defective adenovirus carrying the anti-HBc ScFv gene in vitro and to define the activity of anti-HBc ScFv combining HBcAg. METHODS: The recombinant adenoviruses carrying anti-HBc ScFv gene generated by homologous recombination in bacteria and packaged in 293 cells were transfected into HepG2 cells, and the anti-HBc ScFv was detected using SDS-PAGE and Western blot. RESULTS: Green fluorescent protein (GFP) was observed in HepG2 cells after the transfection. SDS-PAGE displayed a protein strap about 2.7 x 10(4), and the result of Western blot displayed a positive reactive strap. CONCLUSION: Anti-HBc ScFv can be expressed in cells mediated by recombinant replication defective adenovirus carrying the anti-HBc ScFv gene.
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Base de datos:
MEDLINE
Asunto principal:
Adenoviridae
/
Anticuerpos de Cadena Única
/
Anticuerpos contra la Hepatitis B
Idioma:
Zh
Revista:
Zhonghua Gan Zang Bing Za Zhi
Asunto de la revista:
GASTROENTEROLOGIA
Año:
2006
Tipo del documento:
Article