Persistent central memory phenotype of circulating Fel d 1 peptide/DRB1*0101 tetramer-binding CD4+ T cells.
J Allergy Clin Immunol
; 118(6): 1350-6, 2006 Dec.
Article
en En
| MEDLINE
| ID: mdl-17137868
ABSTRACT
BACKGROUND:
Although substantial evidence suggests that T cells are important in the pathogenesis of atopic dermatitis (AD), little is known of the differentiation status of CD4+ T cells specific for common environmental allergens.OBJECTIVE:
To determine the frequency, differentiation phenotype, and function of circulating allergen-specific CD4+ T cells in adult individuals with severe persistent AD and controls.METHODS:
Using tetrameric complexes of an HLA DRB1*0101 restricted epitope from Fel d 1, the major IgE-reactive component of cat dander, we studied ex vivo and cultured T-cell frequency and phenotype in individuals with AD and healthy controls. Cytokine secretion was measured by ex vivo and cultured IFN-gamma, IL-4, and IL-10 enzyme linked immuno-spot analysis.RESULTS:
Ex vivo Fel d 1-specific DRB1*0101-restricted CD4+ T cells express high levels of CCR7, CD62L, CD27, and CD28 and proportionately low levels of tissue-specific homing receptors and TH1 and TH2 cytokine production, placing the cells largely within the central memory subgroup.CONCLUSION:
Circulating Fel d 1-specific DRB1*0101-restricted CD4+ T cells maintain central memory capacity, consistent with a potential to contribute to persisting clinical atopic disease. CLINICAL IMPLICATIONS Persisting central memory characteristics of allergen-specific CD4+ T cells in individuals with AD may contribute to chronic disease.
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Base de datos:
MEDLINE
Asunto principal:
Alérgenos
/
Glicoproteínas
/
Linfocitos T CD4-Positivos
/
Antígenos HLA-DR
/
Dermatitis Atópica
Idioma:
En
Revista:
J Allergy Clin Immunol
Año:
2006
Tipo del documento:
Article