Direct preparation of giant proteo-liposomes by in vitro membrane protein synthesis.
J Biotechnol
; 133(2): 190-5, 2008 Jan 20.
Article
en En
| MEDLINE
| ID: mdl-17900734
ABSTRACT
We investigated the direct constitution of membrane proteins into giant liposomes in cell-free (in vitro) protein synthesis. Giant liposomes were present in a translation reaction cocktail of a wheat germ cell-free protein translation system. Apo cytochrome b(5) (b5) and its fusion proteins were synthesized and directly localized in the liposomes. After the translation reaction, the proteo-liposomes were isolated by simplified discontinuous density-gradient centrifugation. Apo cytochrome b(5) conjugated dihydrofolate reductase (DHFR) was synthesized in the same procedure and the protein was directly displayed on the liposome surface. b5 acts as a "hydrophobic tag" for recruitment to the liposome surface.
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Base de datos:
MEDLINE
Asunto principal:
Biosíntesis de Proteínas
/
Proteínas
/
Citocromos b5
/
Liposomas
Idioma:
En
Revista:
J Biotechnol
Asunto de la revista:
BIOTECNOLOGIA
Año:
2008
Tipo del documento:
Article