Protective effect of vanilloids against tert-butyl hydroperoxide-induced oxidative stress in vero cells culture.
J Agric Food Chem
; 56(10): 3546-53, 2008 May 28.
Article
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| MEDLINE
| ID: mdl-18439017
ABSTRACT
This study investigated the effect of synthetic capsiate, a simplified analogue of capsiate, and vanillyl alcohol on the oxidative stress induced by tert-butyl hydroperoxide (TBH) in a line of fibroblasts derived from monkey kidney (Vero cells). In response to the TBH-mediated oxidative stress, a reduction of the levels of total unsaturated fatty acids and cholesterol was observed, and a rise in the concentrations of conjugated dienes fatty acids hydroperoxides and 7-ketocholesterol. Pretreatment with both synthetic capsiate and vanillyl alcohol preserved Vero cells from oxidative damage and showed a remarkable protective effect on the reduction of the levels of total unsaturated fatty acids and cholesterol, inhibiting the increase of MDA, conjugated dienes fatty acids hydroperoxides, and 7-ketocholesterol. Both compounds were effective against peroxidation of cell membrane lipids induced by TBH, with synthetic capsiate essentially acting as a pro-drug of vanillyl alcohol, its hydrophilic hydrolytic derivative.
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1
Base de datos:
MEDLINE
Asunto principal:
Alcoholes Bencílicos
/
Capsaicina
/
Estrés Oxidativo
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Terc-Butilhidroperóxido
Idioma:
En
Revista:
J Agric Food Chem
Año:
2008
Tipo del documento:
Article