Fluorescence linked immunosorbant assays using microtiter plates.
J Immunol Methods
; 336(2): 135-41, 2008 Jul 31.
Article
en En
| MEDLINE
| ID: mdl-18514691
ABSTRACT
Fluorescence methods are widely used in the detection of antibodies and other binding events. However, as a general screening and detection tool in microtiter plates, enzyme linked immunosorbant (ELISA) methods predominate. In this paper we explore all parameters for effective use of fluorescence as a plate based detection method, including which microtiter plates can be used, the most effective means of immobilization, and the use of different fluorescent dyes or fluorescent proteins. These studies indicate that fluorescent immunosorbant assays (FLISA) can be used as effectively as enzymatic method in microtiter plate based screening methods, including the screening of phage antibody selections.
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Base de datos:
MEDLINE
Asunto principal:
Fluoroinmunoensayo
/
Técnicas de Inmunoadsorción
Idioma:
En
Revista:
J Immunol Methods
Año:
2008
Tipo del documento:
Article