Second international collaborative study evaluating performance characteristics of methods measuring the von Willebrand factor cleaving protease (ADAMTS-13).
J Thromb Haemost
; 6(9): 1534-41, 2008 Sep.
Article
en En
| MEDLINE
| ID: mdl-18662260
ABSTRACT
BACKGROUND:
Over the last 4 years ADAMTS-13 measurement underwent dramatic progress with newer and simpler methods.AIMS:
Blind evaluation of newer methods for their performance characteristics.DESIGN:
The literature was searched for new methods and the authors invited to join the evaluation. Participants were provided with a set of 60 coded frozen plasmas that were prepared centrally by dilutions of one ADAMTS-13-deficient plasma (arbitrarily set at 0%) into one normal-pooled plasma (set at 100%). There were six different test plasmas ranging from 100% to 0%. Each plasma was tested 'blind' 10 times by each method and results expressed as percentage vs. the local and the common standard provided by the organizer.RESULTS:
There were eight functional and three antigen assays. Linearity of observed-vs.-expected ADAMTS-13 levels assessed as r2 ranged from 0.931 to 0.998. Between-run reproducibility expressed as the (mean) CV for repeated measurements was below 10% for three methods, 10-15% for five methods and up to 20% for the remaining three. F-values (analysis of variance) calculated to assess the capacity to distinguish between ADAMTS-13 levels (the higher the F-value, the better the capacity) ranged from 3965 to 137. Between-method variability (CV) amounted to 24.8% when calculated vs. the local and to 20.5% when calculated vs. the common standard. Comparative analysis showed that functional assays employing modified von Willebrand factor peptides as substrate for ADAMTS-13 offer the best performance characteristics.CONCLUSIONS:
New assays for ADAMTS-13 have the potential to make the investigation/management of patients with thrombotic microangiopathies much easier than in the past.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Factor de von Willebrand
/
Conducta Cooperativa
/
Proteínas ADAM
Tipo de estudio:
Clinical_trials
Idioma:
En
Revista:
J Thromb Haemost
Asunto de la revista:
HEMATOLOGIA
Año:
2008
Tipo del documento:
Article