Expression of cyclooxygenase and lipoxygenase enzymes in sinonasal mucosa of patients with cystic fibrosis.
Arch Otolaryngol Head Neck Surg
; 134(8): 825-31, 2008 Aug.
Article
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| MEDLINE
| ID: mdl-18711055
ABSTRACT
OBJECTIVE:
To evaluate the expression of cyclooxygenase (COX) and lipoxygenase (LO) enzymes in the sinonasal mucosa of patients with cystic fibrosis (CF).DESIGN:
Immunohistochemical staining of archived tissue.PARTICIPANTS:
Specimens from 9 patients with CF were analyzed; control specimens were obtained from 4 patients without a history of CF or rhinosinusitis.INTERVENTIONS:
Expression of the enzymes COX-1, COX-2, 5-LO, 12-LO, and 15-LO was evaluated with the use of immunohistochemical techniques in archived sinonasal mucosal tissue from patients with CF. These results were compared with those of the control group.RESULTS:
We noted the characteristic staining patterns of epithelium and submucosal glands for each enzyme. Statistically significant (P < .05) differences between control and CF specimens were noted in the staining intensity of columnar epithelium for COX-2 (cytoplasm) and 12-LO (cytoplasm and nucleus) and of submucosal glands for COX-2 (cytoplasm) and 12-LO (cytoplasm). No significant differences were noted for the staining intensity of COX-1, 5-LO, or 15-LO between the groups.CONCLUSIONS:
Significant differences in sinonasal mucosal expression of COX-2 and 12-LO enzymes exist between patients with CF and controls. This suggests a difference in arachidonic acid metabolism between these 2 groups.
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Base de datos:
MEDLINE
Asunto principal:
Sinusitis
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Rinitis
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Lipooxigenasa
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Prostaglandina-Endoperóxido Sintasas
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Fibrosis Quística
Idioma:
En
Revista:
Arch Otolaryngol Head Neck Surg
Asunto de la revista:
OTORRINOLARINGOLOGIA
Año:
2008
Tipo del documento:
Article