Aptasensors for detection of microbial and viral pathogens.
Biosens Bioelectron
; 24(11): 3175-82, 2009 Jul 15.
Article
en En
| MEDLINE
| ID: mdl-19117748
ABSTRACT
Aptamers are specific nucleic acid sequences that can bind to a wide range of non-nucleic acid targets with high affinity and specificity. These molecules are identified and selected through an in vitro process called SELEX (systematic evolution of ligands by exponential enrichment). Proteins are the most common targets in aptamer selection. In diagnostic and detection assays, aptamers represent an alternative to antibodies as recognition agents. Cellular detection is a promising area in aptamer research. One of its principal advantages is the ability to target and specifically differentiate microbial strains without having previous knowledge of the membrane molecules or structural changes present in that particular microorganism. The present review focuses on aptamers, SELEX procedures, and aptamer-based biosensors (aptasensors) for the detection of pathogenic microorganisms and viruses. Special emphasis is placed on nanoparticle-based platforms.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Bacterias
/
Virus
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Técnicas Biosensibles
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Aptámeros de Nucleótidos
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Técnica SELEX de Producción de Aptámeros
Tipo de estudio:
Diagnostic_studies
/
Systematic_reviews
Idioma:
En
Revista:
Biosens Bioelectron
Asunto de la revista:
BIOTECNOLOGIA
Año:
2009
Tipo del documento:
Article